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  4. Recombinant Human Tyrosine 3-monooxygenase (TH)

Recombinant Human Tyrosine 3-monooxygenase (TH)

  • 中文名稱：
    人TH重組蛋白
  • 貨號：
    CSB-YP023470HU
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    人TH重組蛋白
  • 貨號：
    CSB-EP023470HU
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    人TH重組蛋白
  • 貨號：
    CSB-EP023470HU-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    人TH重組蛋白
  • 貨號：
    CSB-BP023470HU
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    人TH重組蛋白
  • 貨號：
    CSB-MP023470HU
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    Dystonia 14; DYT14; DYT5b; EC 1.14.16.2; OTTHUMP00000011225; OTTHUMP00000011226; ple; Protein Pale; TH; The; TY3H_HUMAN; TYH; Tyrosine 3 hydroxylase; Tyrosine 3 monooxygenase; Tyrosine 3-hydroxylase; Tyrosine 3-monooxygenase; Tyrosine hydroxylase
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達(dá)區(qū)域：
    2-528
  • 氨基酸序列
    PTPDATTPQ AKGFRRAVSE LDAKQAEAIM VRGQGAPGPS LTGSPWPGTA APAASYTPTP RSPRFIGRRQ SLIEDARKER EAAVAAAAAA VPSEPGDPLE AVAFEEKEGK AVLNLLFSPR ATKPSALSRA VKVFETFEAK IHHLETRPAQ RPRAGGPHLE YFVRLEVRRG DLAALLSGVR QVSEDVRSPA GPKVPWFPRK VSELDKCHHL VTKFDPDLDL DHPGFSDQVY RQRRKLIAEI AFQYRHGDPI PRVEYTAEEI ATWKEVYTTL KGLYATHACG EHLEAFALLE RFSGYREDNI PQLEDVSRFL KERTGFQLRP VAGLLSARDF LASLAFRVFQ CTQYIRHASS PMHSPEPDCC HELLGHVPML ADRTFAQFSQ DIGLASLGAS DEEIEKLSTL YWFTVEFGLC KQNGEVKAYG AGLLSSYGEL LHCLSEEPEI RAFDPEAAAV QPYQDQTYQS VYFVSESFSD AKDKLRSYAS RIQRPFSVKF DPYTLAIDVL DSPQAVRRSL EGVQDELDTL AHALSAIG
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Plays an important role in the physiology of adrenergic neurons. Positively regulates the regression of retinal hyaloid vessels during postnatal development.
  • 基因功能參考文獻(xiàn)：
    1. These results provide a novel mechanism of how NO can modulate TH's enzymatic activity through S-nitrosylation. PMID: 28287127
    2. It is a genetic risk for Parkinson's disease. PMID: 29724574
    3. One novel mutation of c.679A>G (p.T227A) in GCH1 and 3 known mutations of c.457C>T (p.R153X), c.739G>A (p.G247S), and c.698G>A (p.R227H) in tyrosine hydroxylase (TH) have been found and predicted to be damaging or deleterious. PMID: 29405179
    4. This study does not support that early-onset PD may be the male presentation of TH deficiency attributed to this founder mutation in Greek patients. PMID: 27666733
    5. A novel heterozygous variant in tyrosine hydroxylase was identified in Chinese patients with dopa-responsive dystonia. PMID: 27619486
    6. This study indicates that mutations in TH are rare in late-onset Parkinson's disease. PMID: 27185167
    7. The purpose of this study is to investigate the clinical significance of tyrosine hydroxylase (TH) expression in peripheral blood (PB) at diagnosis in patients with neuroblastoma.. The treatment intensity should be tailored according to TH expression in PB at diagnosis. PMID: 27034145
    8. Our results suggest that the TH-immunoreactive cells in the human cortex do not overlap with any known neurochemically-defined subsets of interneurons and provide further evidence of differences in the phenotype of these cells across species. PMID: 27448941
    9. Results show that the positive rates and expression levels of nestin, tyrosine hydroxylase (TH), GFAP and IL-17 were significantly decreased while Foxp3 and the ratio of Foxp3/IL-17 were statistically elevated in BM of AML patients. PMID: 27016413
    10. Data suggest that TH phosphorylated at Ser-31 co-distributes with Golgi complexes and synaptic-like vesicles in rat and human dopaminergic neurons/cell lines; Ser-31 phosphorylation may regulate TH subcellular localization by enabling its transport along microtubules, notably toward the projection terminals. PMID: 28637871
    11. TH is a robust interaction partner of different 14-3-3 dimer types with moderate variability between the 14-3-3 dimers on their regulation of TH. PMID: 26825549
    12. Germline mutations in the TH gene are linked to Familial isolated pituitary adenoma in a Brazilian Family. PMID: 27245436
    13. No statistically significant differences were found between cases and controls for the allele frequencies in five genes: TH, SLC18A2, DRD1, DRD3 and COMT. Conversely, some alleles of the 12 sNPs from the DRD2 locus and the 5 from the MAOA locus showed significant associations with excessive alcohol consumption. PMID: 26447226
    14. Results show that metastasis-associated protein 1 (MTA1) and tyrosine hydroxylase (TH) levels were significantly down-regulated in Parkinson disease (PD) samples as compared with normal brain tissue PMID: 27044752
    15. the reduction of tyrosine hydroxylase-immunoreactive neurons occurring in the locus coeruleus after perinatal hypoxic insults persists into adulthood PMID: 26647061
    16. The data suggest that presence of a homozygous V81M polymorphism is associated with more severe freezing of gait in patients with Parkinson's disease PMID: 26732803
    17. In this study we found that TH protein levels did not differ between control and schizophrenia groups in the nucleus accumbens. PMID: 26386900
    18. In high-risk metastatic Neuroblastoma, TH and DCX mRNA quantification could be used for the assessment of response to treatment and for early detection of progressive disease or relapses. PMID: 26498952
    19. the allelic frequency of the TH01 marker in 171 Swiss sudden infant death syndrome (SIDS) infants and 500 healthy and gender-matched Caucasian adults showed that the 9.3 allele is similarly distributed in SIDS cases and controls (27.2% vs. 25.6%; p-value = 0.562). PMID: 24975687
    20. This study showed that a new tyrosine hydroxylase knock-in mouse model of l-DOPA-responsive dystonia. PMID: 26220941
    21. The mutant tyrosine hydroxylase enzyme was unstable and exhibited deficient stabilization by catecholamines, leading to decline of brain tyrosine hydroxylase-immunoreactivity in the Th knock-in mice. PMID: 26276013
    22. Thus, the hTH-GFP reporter rat should be a valuable tool for Parkinson's disease research. PMID: 25462571
    23. A detailed analysis of the interaction between singly or doubly phosphorylated human tyrosine hydroxylase isoform 1(1-50) peptides and 14-3-3zeta PMID: 25418103
    24. Study found evidence that DNA variation in the ADRA2A gene may be causally related to ADHD-like behaviors, and for a novel association between a TH gene variant and intra-individual variability PMID: 24166412
    25. Proteomics analysis show that Ser40 of TH protein does not significantly contribute to the binding of 14-3-3gamma, and rather has reduced accessibility in the TH:14-3-3gamma complex. PMID: 24947669
    26. increased expression of TH and GAP43 might be a molecular mechanism for left atrial myoelectricity remodeling of aging atrial fibrillation patients, which might be potential therapeutic targets of atrial fibrillation. PMID: 24301786
    27. biosynthesis of catecholamine by the action of TH should be deeply involved in decreased intellectual ability in patients with schizophrenia PMID: 24417771
    28. A297, E362/E365 and S368 of TH were shown to mediate high affinity dopamine inhibition through V(max) reduction and increasing the K(M) for the cofactor. PMID: 24334288
    29. Tyrosine hydroxylase polymorphisms contribute to attempted suicide in schizophrenia. PMID: 24275212
    30. Neurons of the substantia nigra from the Lesch-Nyhan disease cases show reduced melanization and reduced reactivity for tyrosine hydroxylase (TH), the rate-limiting enzyme in dopamine synthesis. PMID: 24891139
    31. Achilles tendon tenocytes produce tyrosine hydroxylase. PMID: 22292987
    32. In a South African cohort, Africans had a higher incidence of hypertension and higher occurrence of the C-824T TH mutation. However, the contribution of the tyrosine hydroxylase C-824T polymorphism to hypertension could not be confirmed. PMID: 23489065
    33. Nurr1 overexpression significantly increased the SIRT1 occupancy of the consensus elements for Nurr1 binding hTH promoter region. PMID: 23977047
    34. The region surrounding pSer19 of Tyrosine hydroxylase adopts an extended conformation in the 14-3-3gamma-bound state, whereas adopts a bent conformation when free in solution. PMID: 24055376
    35. Data suggest that coordination of nitric oxide to Fe(II) in TyrH is directed by presence of tetrahydropterin at active site, binding in a fashion that may be important for directing first step of catalytic cycle toward hydroxylation of tyrosine. PMID: 24168553
    36. In 10 sporadic cases of dopa-responsive dystonia, only two heterozygous tyrosine hydroxylase mutations (Ser19Cys and Gly397Arg) were found in two subjects with unknown pathogenicity. PMID: 23762320
    37. Data indicate that the C-terminal domain was the immunodominant part of tryptophan hydroxylase TPH1, the epitopes of tryptophan hydroxylase TPH2 and tyrosine hydroxylase (TH) were mainly located in the N-terminal regulatory domains. PMID: 23182718
    38. our studies have clearly identified a glucocorticoid-responsive element in a 7 bp AP-1-like motif in the promoter region at -7.24 kb of the human TH gene PMID: 23647419
    39. In severe prolonged fetal hypoxia, there was a striking reduction or absence of tyrosine hydroxylase in all the mesencephalic nuclei. PMID: 23481708
    40. This review discusses the current understandings on the genetic variants in TH and their correlations with Parkinson's disease. PMID: 22583432
    41. This study presented a THD family with predominant myoclonus-dystonia and a new genotype. PMID: 22815559
    42. molecular analysis revealed two novel heterozygous mutations c.636A>C and c.1124G>C in the TH gene PMID: 22691284
    43. mRNA expressions of AQP4 and TH were found to be reduced whereas that of PBP was found to be elevated when compared with those of healthy control samples PMID: 22083667
    44. Data show calbindin (CB)- and tyrosine hydroxylase (TH)-cells were distributed in the three striatal territories, and the density of calretinin (CR) and parvalbumin (PV) interneurons were more abundant in the associative and sensorimotor striatum. PMID: 22272358
    45. Protein levels for tyrosine hydroxylase peaked during the first year of life then gradually declined to adulthood. PMID: 22336227
    46. data indicate that ligand-bound PR-B is recruited to DNA elements in the TH promoter and acts as a transcriptional activator of the TH gene PMID: 21815951
    47. these results suggest that region-specific methylation and methyl-CpG binding domain proteins play important roles in TH gene regulation in neural stem cells. PMID: 22001923
    48. Human RXRalpha interacts with and represses Nurr1-dependent transcriptional activation in tyrosine hydroxylase (TH)-expressing dopaminergic neuronal stem cells in culture, downregulating TH promoter activity. PMID: 22066143
    49. Data indicate that TH gene expression can be regulated by alpha-synuclein (alpha-SYN); further, interference with TH gene expression through elevated levels of alpha-SYN could be associated with dopaminergic neuronal dysfunction. PMID: 21656370
    50. Data from samples of centenarians, nonagenarians and younger controls suggest that the TH01 STR locus exhibits no significant influence on the ability of attaining exceptional old age in Germans. PMID: 21407269

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  • 相關(guān)疾病：
    Segawa syndrome autosomal recessive (ARSEGS)
  • 亞細(xì)胞定位：
    Cytoplasm, perinuclear region.
  • 蛋白家族：
    Biopterin-dependent aromatic amino acid hydroxylase family
  • 組織特異性：
    Mainly expressed in the brain and adrenal glands.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 11782

    OMIM: 191290

    KEGG: hsa:7054

    STRING: 9606.ENSP00000370571

    UniGene: Hs.435609