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  4. Recombinant Human Ubiquitin-conjugating enzyme E2 T (UBE2T)

Recombinant Human Ubiquitin-conjugating enzyme E2 T (UBE2T)

  • 中文名稱：
    人UBE2T重組蛋白
  • 貨號：
    CSB-YP878841HU
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    人UBE2T重組蛋白
  • 貨號：
    CSB-EP878841HU
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    人UBE2T重組蛋白
  • 貨號：
    CSB-EP878841HU-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    人UBE2T重組蛋白
  • 貨號：
    CSB-BP878841HU
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    人UBE2T重組蛋白
  • 貨號：
    CSB-MP878841HU
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    UBE2T
  • Uniprot No.：
  • 別名：
    Cell proliferation inducing gene 50 protein; Cell proliferation-inducing gene 50 protein; HSPC150; HSPC150 protein similar to ubiquitin conjugating enzyme; PIG50; Ube2t; UBE2T_HUMAN; Ubiquitin carrier protein T; Ubiquitin conjugating enzyme; ubiquitin conjugating enzyme E2 T; Ubiquitin conjugating enzyme E2T; Ubiquitin protein ligase T; Ubiquitin-conjugating enzyme E2 T; Ubiquitin-protein ligase T
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    full length protein
  • 表達區(qū)域：
    1-197
  • 氨基酸序列
    MQRASRLKRE LHMLATEPPP GITCWQDKDQ MDDLRAQILG GANTPYEKGV FKLEVIIPER YPFEPPQIRF LTPIYHPNID SAGRICLDVL KLPPKGAWRP SLNIATVLTS IQLLMSEPNP DDPLMADISS EFKYNKPAFL KNARQWTEKH ARQKQKADEE EMLDNLPEAG DSRVHNSTQK RKASQLVGIE KKFHPDV
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in mitomycin-C (MMC)-induced DNA repair. Acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCL and FANCI. May contribute to ubiquitination and degradation of BRCA1. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination.
  • 基因功能參考文獻：
    1. Results show that UBE2T expression was increased in gastric tumors. Its suppression altered the expression of epithelial-mesenchymal transition factors and inhibited growth and colony formation, increased G2/M phase cell cycle arrest, and promoted apoptosis in gastric cancer cells. These findings provide evidence that UBE2T plays a critical role in gastric cancer. PMID: 28427240
    2. High UBE2T expression is associated with nasopharyngeal carcinoma. PMID: 26943030
    3. Collectively, our findings suggest UBE2T serves as a promising prognostic factor for HCC and functions as an oncogene. The newly identified miR-543/UBE2T/p53 axis may represent a new potential therapeutic target for HCC intervention. PMID: 28935368
    4. report the identification of a new allosteric pocket on Ube2T through a fragment screening using biophysical methods. Several fragments binding to this site inhibit ubiquitin conjugation in vitro PMID: 28437106
    5. Results showed that UBE2T was overexpressed in osteosarcoma tissues and cell lines. Moreover, UBE2T knockdown inhibited osteosarcoma cell proliferation, migration, and invasion. PMID: 27712593
    6. UBE2T plays an important role in the tumorigenesis of gastric cancer and could act as a potential independent prognostic factor for cancer therapy. PMID: 27020591
    7. Identify critical roles of UBE2T in prostate cancer development and progression. PMID: 26308072
    8. we identified UBE2T as a bona fide FA gene (FANCT) that also may be a rare cancer susceptibility gene. PMID: 26085575
    9. UBE2T is the primary E2 of the FA pathway required for FANCD2 and FANCI activation. PMID: 26119737
    10. Mutations in the gene encoding the E2 conjugating enzyme UBE2T cause Fanconi anemia PMID: 26046368
    11. hypoxic conditions down-regulate UBE2T expression which correlates with an increased sensitivity to crosslinking agents consistent with a defective Fanconi anemia pathway PMID: 21722982
    12. UBE2T is the ubiquitin-conjugating enzyme (E2) in the Fanconi anemia pathway and has a self-inactivation mechanism that could be important for negative regulation of the Fanconi anemia pathway. PMID: 16916645
    13. UBE2T was significantly upregulated in lung cancer tissue and cell lines, suggesting involvement of UBE2T in the malignant cell phenotype. PMID: 18667844
    14. This work therefore establishes a system that provides mechanistic insight into the functions of FANCL and FANCI in the catalysis of FANCD2 monoubiquitination. PMID: 19111657
    15. Upon the occurrence of DNA damage, FANCI becomes monoubiquitinated on Lys-523 by the UBE2T-FANCL pair. PMID: 19589784
    16. Our data imply a critical role of UBE2T in development and/or progression of breast cancer through the interaction with and the regulation of the BRCA1/BARD1 complex. PMID: 19887602

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  • 相關(guān)疾病：
    Fanconi anemia complementation group T (FANCT)
  • 亞細胞定位：
    Nucleus. Note=Accumulates to chromatin.
  • 蛋白家族：
    Ubiquitin-conjugating enzyme family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 25009

    OMIM: 610538

    KEGG: hsa:29089

    STRING: 9606.ENSP00000356243

    UniGene: Hs.5199