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  4. Recombinant Human Vitronectin (VTN)

Recombinant Human Vitronectin (VTN)

  • 中文名稱：
    人VTN重組蛋白
  • 貨號：
    CSB-EP025944HU
  • 規(guī)格：
    ￥2532
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 別名：
    S-protein (Serum-spreading factor) (V75)
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Full Length of Mature Protein
  • 來源：
    E.coli
  • 分子量：
    52.4 kDa
  • 表達區(qū)域：
    20-478aa
  • 氨基酸序列
    DQESCKGRCTEGFNVDKKCQCDELCSYYQSCCTDYTAECKPQVTRGDVFTMPEDEYTVYDDGEEKNNATVHEQVGGPSLTSDLQAQSKGNPEQTPVLKPEEEAPAPEVGASKPEGIDSRPETLHPGRPQPPAEEELCSGKPFDAFTDLKNGSLFAFRGQYCYELDEKAVRPGYPKLIRDVWGIEGPIDAAFTRINCQGKTYLFKGSQYWRFEDGVLDPDYPRNISDGFDGIPDNVDAALALPAHSYSGRERVYFFKGKQYWEYQFQHQPSQEECEGSSLSAVFEHFAMMQRDSWEDIFELLFWGRTSAGTRQPQFISRDWHGVPGQVDAAMAGRIYISGMAPRPSLAKKQRFRHRNRKGYRSQRGHSRGRNQNSRRPSRATWLSLFSSEESNLGANNYDDYRMDWLVPATCEPIQSVFFFSGDKYYRVNLRTRRVDTVDPPYPRSIAQYWLGCPAPGHL
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標簽：
    Tag-Free
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    13-23 business days
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.; Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
  • 基因功能參考文獻：
    1. Insulin resistance and age independently predicted VTN levels. VTN may be a candidate target for the appraisal of hepatic insulin resistance in patients with type 2 diabetes. PMID: 29780059
    2. The 10 kDa C-terminal fragment of the ECM protein vitronectin (VTN) was then selected as a promising circulating biomarker in discriminating Nonalcoholic steatohepatitis. PMID: 29463024
    3. The authors observed that exposure of Burkholderia pseudomallei and the closely related yet avirulent Burkholderia thailandensis to human plasma increased epithelial cell invasion by >20 fold. Receptor blocking studies with RGD-domain containing peptide and alphaV beta3 blocking antibody identified complement-activated vitronectin as the factor facilitating this invasion. PMID: 28186697
    4. Additionally, the authors utilized vitroenctin-derived peptides to map the minimal Rickettsia conorii Adr1/human vitronectin interaction to the C-terminal region of vitronectin. PMID: 28299286
    5. We show that small N-terminal fragments of VN consistent with processing of the RGD motif are present in liquid biopsies of cancer patients as well as in the urine of healthy individuals. PMID: 27189837
    6. Serum vitronectin levels are elevated in human glioma, as well as are independently associated with pathologic grade based on WHO classification and 5-year poor prognosis. Moreover, this biomarker significantly discriminates gliomas from controls, other non-glioma brain tumors and other non-tumor neurological diseases, distinguishes high-grade gliomas from low-grade gliomas, and predicts 5-year progression and mortality. PMID: 27871448
    7. Elevated serum VN levels represented high diagnostic value and had close relation to clinicopathological factors and early recurrence, suggesting that serum VN might be a useful diagnostic and prognostic marker for HCC. PMID: 27802203
    8. We confirmed increased levels of C1R and VTN in sera from patients with Joint hypermobility syndrome by western blot analyses PMID: 26709396
    9. This study examines the complexes of PAI-1 with tissue-type and urokinase-type plasminogen activator and vitronectin revealed by changes in the conformation and dynamics of the reactive center loop. PMID: 26548921
    10. Ail-expressing Yersinia pestis strains bind vitronectin - a host protein with functions in cell attachment, fibrinolysis and inhibition of the complement system. PMID: 26377177
    11. High expression of VN in tumor cells independently indicated poor clinical prognosis in patients with osteosarcoma. PMID: 26617861
    12. The mean level of PAI-1 was 23.02 +/- 15 (8.2-71.19) ng/mL and vitronectin was 83.10% +/- 23.77% (12%-126%) in the tumor group. PMID: 24770328
    13. Bacterial protein-E and host vitronectin play a role in the attachment to bronchial epithelial cells and is also involved in the subsequent intracellular invasion of nontypeable Haemophilus influenzae. PMID: 26572616
    14. P. aeruginosa isolates obtained from CF patients significantly bound vitronectin. Porin D was defined as a novel P. aeruginosa vitronectin-receptor PMID: 26047937
    15. Lpd of P. aeruginosa is a surface exposed moonlighting protein that binds two human terminal pathway inhibitors, vitronectin and clusterin PMID: 26368530
    16. The VN level may be relevant as a clinical biomarker for adverse cardiovascular outcomes not only in patients with ischemic heart disease undergoing coronary interventions. PMID: 24823429
    17. Vitronectin expression in the airways of subjects with asthma and chronic obstructive pulmonary disease PMID: 25768308
    18. Gpm1 in Candida albicans attaches to human endothelial cells and to keratinocytes via the adhesive protein vitronectin PMID: 24625558
    19. Vitronectin is identified as a common constituent of amyloid deposits in 175 formalin-fixed and paraffin-embedded tissue samples from a large variety of different amyloid diseases. PMID: 26101327
    20. Hic interacts with vitronectin and simultaneously with Factor H, and both human proteins may contribute to colonisation and invasive disease caused by serotype 3 pneumococci. PMID: 25181963
    21. VTN plays a significant role in the malignant growth of tumor. PMID: 25179307
    22. Melanoma cell adhesion to vitronectin is dependent on beta-1,6-branched N-glycans. PMID: 24687613
    23. A total of 17 site-specific N-glycopeptides were completely identified in all of the three N-glycosylation sites of vitronectin in human plasma, including 12 N-glycopeptides first reported. PMID: 25374123
    24. The presence of VN on the material surfaces enhanced cell-mediated FN reorganization and secretion. PMID: 23899674
    25. serum levels of both fibronectin and vitronectin may be the diagnostic markers in melanoma patients, but not markers of prognosis PMID: 24999757
    26. Authors demonstrate that an evolutionarily conserved rickettsial antigen, Adr1/RC1281, interacts with human vitronectin and is sufficient to mediate resistance to serum killing when expressed at the outer-membrane. PMID: 24286496
    27. The interaction between uPAR and vitronectin triggers ligand-independent adhesion signalling by integrins. PMID: 25168639
    28. A strong linkage disequilibrium was found in vitronectin promoter in Chinese with vascular disease. PMID: 23041018
    29. Vn competes with hTSP-1 for binding to Atl repeats and vice versa. In conclusion, this study identifies the Atl repeats as bacterial adhesive structures interacting with the human glycoproteins hTSP-1 and Vn. PMID: 24371140
    30. increased expression levels of VN protein and mRNA in placental maternal-fetal interface of EOSP may be involved in adhesion and repair of placental infarct lesions. PMID: 23124223
    31. Interaction of vitronectin with integrin alphavbeta3 results in the continued activation of the kinase mTOR. PMID: 23722547
    32. In conclusion, the head domains of UspA2/2H have extensive sequence polymorphism without losing vitronectin-binding capacity promoting a general evasion of the host immune system. PMID: 23474333
    33. study highlights that ascites-derived fibronectin and vitronectin exhibit different properties depending on the ascites PMID: 23811340
    34. An isogenic nontypeable Haemophilus influenzae 3655Deltahpf mutant devoid of protein F displayed a reduced binding of vitronectin, and was consequently more sensitive to killing by human serum compared with the wild type. PMID: 23387957
    35. The choline-binding protein PspC of Streptococcus pneumoniae interacts with the C-terminal heparin-binding domain of vitronectin. PMID: 23603906
    36. Importance of vitronectin and its interaction with the urokinase receptor in tumor growth. PMID: 23327926
    37. There was no difference in serum vitronectin levels between Behcet's disease patients and control subjects, and there was no statistically significant difference between vitronectin levels in Behcet patients with and without ocular involvement. PMID: 22810367
    38. In this study, the authors demonstrate meningococcal interactions with vitronectin via a novel adhesin, Msf (meningococcal surface fibril, previously NhhA or Hsf). PMID: 22050461
    39. higgh plasms levels is associated with the risk of metabolic syndrome and type 2 diabetes mellitus PMID: 21800006
    40. A series of truncated vitronectin molecules revealed that the C-terminal domain comprising vitronectin(353-363) harboured the major binding region for Haemophilus influenzae protein E. PMID: 21542857
    41. High serum vitronectin is associated with breast cancer. PMID: 21253761
    42. PAI-2 is capable of inhibiting uPA in the presence of PAI-1, particularly on adherent cells in the presence of vitronectin PMID: 21316840
    43. Thrombospondin 1, fibronectin, and vitronectin are differentially dependent upon RAS, ERK1/2, and p38 for induction of vascular smooth muscle cell chemotaxis. PMID: 21193465
    44. The results provide the first evidence for vitronectin gene expression in urothelial cells. PMID: 21048021
    45. Plasminogen activator inhibitor-1 and vitronectin expression level and stoichiometry regulate vascular smooth muscle cell migration through physiological collagen matrices PMID: 20492459
    46. expression of vitronectin at the periphery of mesothelial cells and within ovarian cancer cell clusters suggests a potential role for this molecule during intraperitoneal implantation of ovarian cancer cells PMID: 20121701
    47. This study directly reveals the binding mode between the N-terminal domain of UspA2 Moraxella catarrhalis and the C-terminal part of vitronectin and thus sheds light upon the mechanism of Moraxella catarrhalis-dependent serum resistance. PMID: 20199596
    48. Data reveal that multimeric VTNC attenuate Cyr61 binding to immobilized VTNC, while monomeric VTNC was ineffective. PMID: 20195466
    49. A fragment of vitronectin containing the RGD integrin binding site showed similar binding affinity as that of full vitronectin protein to purified integrin alphavbeta3 but had diminished cell adhesion and spreading function in vivo. PMID: 20600001
    50. Neisseria meningitidis Opc expressed in acapsulate as well as capsulate bacteria can increase human brain endothelial cell line adhesion and entry by first binding to serum vitronectin PMID: 20502634

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  • 亞細胞定位：
    Secreted, extracellular space.; Parasitophorous vacuole.
  • 組織特異性：
    Expressed in the retina pigment epithelium (at protein level). Expressed in plasma (at protein level). Expressed in serum (at protein level).
  • 數(shù)據(jù)庫鏈接：

    HGNC: 12724

    OMIM: 193190

    KEGG: hsa:7448

    STRING: 9606.ENSP00000226218

    UniGene: Hs.2257