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  4. Recombinant Human herpesvirus 1 Deneddylase UL36 (UL36), partial

Recombinant Human herpesvirus 1 Deneddylase UL36 (UL36), partial

  • 中文名稱：
    Recombinant Human herpesvirus 1 Deneddylase UL36 (UL36) ,partial,Yeast
  • 貨號：
    CSB-YP320729HWY
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Human herpesvirus 1 Deneddylase UL36 (UL36) ,partial,Yeast
  • 貨號：
    CSB-EP320729HWY
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Human herpesvirus 1 Deneddylase UL36 (UL36) ,partial,Yeast
  • 貨號：
    CSB-EP320729HWY-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Human herpesvirus 1 Deneddylase UL36 (UL36) ,partial,Yeast
  • 貨號：
    CSB-BP320729HWY
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Human herpesvirus 1 Deneddylase UL36 (UL36) ,partial,Yeast
  • 貨號：
    CSB-MP320729HWY
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    UL36
  • Uniprot No.：
  • 別名：
    UL36Large tegument protein deneddylase; EC 3.4.19.12; EC 3.4.22.-
  • 種屬：
    Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Large tegument protein that plays multiple roles in the viral cycle. During viral entry, remains associated with the capsid while most of the tegument is detached and participates in the capsid transport toward the host nucleus. Plays a role in the routing of the capsid at the nuclear pore complex and subsequent uncoating. Within the host nucleus, acts as a deneddylase and promotes the degradation of nuclear CRLs (cullin-RING ubiquitin ligases) and thereby stabilizes nuclear CRL substrates, while cytoplasmic CRLs remain unaffected. These modifications prevent host cell cycle S-phase progression and create a favorable environment allowing efficient viral genome replication. Participates later in the secondary envelopment of capsids. Indeed, plays a linker role for the association of the outer viral tegument to the capsids together with the inner tegument protein.
  • 基因功能參考文獻：
    1. The findings underline the roles of UL36USP-IFNAR2 interaction in counteracting the type I IFN-mediated signaling pathway and reveal a novel evasion mechanism of antiviral innate immunity by HSV-1. PMID: 29997210
    2. UL36USP deubiquitinating activity supports HSV-1 virus replication during infection. PMID: 28348081
    3. UL36 abrogates NF-kappaB activation by cleaving polyubiquitin chains from IkappaBalpha and therefore restricts proteasome-dependent degradation of IkappaBalpha and that DUB activity is indispensable for this process. PMID: 28031360
    4. characterized 2 conserved tryptophan-acidic motifs in the central region of pUL36; results indicate the unique conserved tryptophan-acidic motifs in the central region of pUL36 are required for efficient targeting of progeny capsids to the membranes of secondary capsid envelop PMID: 27009950
    5. UL36p-independent binding of capsids to organelles occurs prior to the function of UL36p in capsid envelopment. PMID: 26339048
    6. an essential feature of UL36 is the existence in its central region of a stalk capable of connecting capsid and membrane across the tegument and that the ability to switch between monomeric and dimeric forms may help UL36 fulfill its multiple functions PMID: 25678705
    7. Authors showed that in contrast to accepted models, the capsid vertex-specific component is not formed from pUL17 and pUL25 on their own but requires a contribution from pUL36. PMID: 25410861
    8. In addition, immunoblot analysis suggested that failure to bind pUL37 affected the stability of pUL36. PMID: 24725933
    9. These findings demonstrate that herpes simplex virus 1 ubiquitin-specific protease UL36 removes polyubiquitin chains on TRAF3 and counteracts the IFN-beta pathway. PMID: 23986588
    10. Cytosolic herpes simplex virus capsids not only require binding inner tegument protein pUL36 but also pUL37 for active transport prior to secondary envelopment. PMID: 23186167
    11. Nuclear localization signal in VP1-2(UL36) is essential for infection via capsid routing to the nuclear pore. PMID: 22718835
    12. UL36 plays a major role in the tegumentation of the virion, providing a flexible scaffold to which other tegument proteins, including UL37, bind. PMID: 22345483
    13. 2430 to 2893 of HSV1 pUL36, containing one binding site for the capsid protein pUL25, are sufficient to recruit pUL36 onto cytosolic capsids during assembly. PMID: 22258258
    14. Interaction between VP1/2 and VP16 is important for viral assembly but is not essential for HSV-1 replication in cell culture. PMID: 22013045
    15. Here the authors show that herpes simplex virus 1 VP1-2 ubiquitin-specific protease can act on itself and is important for stability. PMID: 21715485
    16. A single mutation in the UL36 protein is responsible for virus temperature-sensitive entry and assembly defects. PMID: 21177812
    17. The findings are interpreted to indicate that UL36 and UL37 are the components of the tufts and of the thin strands that extend from them. PMID: 20631146
    18. For each HSV-1 mutant, analysis of purified deenveloped C capsids indicated that only in the absence of pUL36 was there a complete loss of capsid-bound pUL48, as well as pUL37 PMID: 19923173
    19. Single changes to alanine of pUL36 residues F593 and E596 impaired binding of pUL37 with the greatest effect observed for the substitution E596A. PMID: 17651773
    20. we propose a model in which after the herpes simplex virus (HSV) capsid docks at the nuclear pore, the tegument protein attached to the capsid must be cleaved by a serine or a cysteine protease in order for the DNA to be released into the nucleus. PMID: 18216103
    21. These findings suggest that UL36p is necessary for HSV-1 anterograde transport. PMID: 18495763
    22. cells infected with UL37/deltaUL36 virus showed that, in the absence of UL36, accumulation of UL37 at the Golgi complex was not evident. PMID: 18787001
    23. these inner tegument proteins have differing functions, with pUL36 being essential during both the assembly and uptake stages of infection, while pUL37 is needed for the formation of virions but is not required during the initial stages of infection. PMID: 18971278

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  • 亞細胞定位：
    Virion tegument. Host cytoplasm. Host nucleus.
  • 蛋白家族：
    Herpesviridae large tegument protein family
  • 數(shù)據(jù)庫鏈接：

    KEGG: vg:2703357