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  4. Recombinant Lactococcus lactis subsp. lactis Multidrug resistance ABC transporter ATP-binding and permease protein (lmrA), partial

Recombinant Lactococcus lactis subsp. lactis Multidrug resistance ABC transporter ATP-binding and permease protein (lmrA), partial

  • 中文名稱：
    乳酸乳球菌亞屬。乳酸lmrA重組蛋白
  • 貨號：
    CSB-YP880302LNG1
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    乳酸乳球菌亞屬。乳酸lmrA重組蛋白
  • 貨號：
    CSB-EP880302LNG1
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    乳酸乳球菌亞屬。乳酸lmrA重組蛋白
  • 貨號：
    CSB-EP880302LNG1-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    乳酸乳球菌亞屬。乳酸lmrA重組蛋白
  • 貨號：
    CSB-BP880302LNG1
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    乳酸乳球菌亞屬。乳酸lmrA重組蛋白
  • 貨號：
    CSB-MP880302LNG1
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    lmrA
  • Uniprot No.：
  • 別名：
    lmrA; LL0711; L116532; Multidrug resistance ABC transporter ATP-binding and permease protein
  • 種屬：
    Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Efflux transporter for a variety of amphiphilic cationic compounds, including antibiotics.
  • 基因功能參考文獻：
    1. analysis of the effect of detergents on the activity of the ABC transporter LmrA PMID: 21651889
    2. a carboxylate has a critical role in proton conduction by the ATP-binding cassette multidrug transporter LmrA PMID: 16040836
    3. LmrA-mediated Hoechst 33342 transport in membrane vesicles is influenced by the transmembrane pH gradient due to a pH-dependent partitioning of Hoechst 33342 into the membrane. PMID: 16363806
    4. Remodelling of LmrA using the atomic coordinates of the MsbA homologue Sav1866 from Staphylococcus aureus is reported. PMID: 17624317
    5. The membrane domain of LmrA mediates an ethidium/proton co-transport reaction, suggesting a functional similarity in the mechanism of secondary-active and ABC transporters. PMID: 14685244
    6. LmrA mediates the reverse uptake of ethidium in ATP-depleted cells in the presence of an inwardly directed ethidium concentration gradient and LmrA-mediated uptake of ethidium is coupled to the catalytic cycle in the direction of ATP synthesis PMID: 14660649

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  • 亞細胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    ABC transporter superfamily, Multidrug exporter LmrA (TC 3.A.1.117.1) family
  • 數據庫鏈接：

    KEGG: lla:L116532

    STRING: 272623.L116532