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Recombinant Mouse Angiopoietin-like protein 8 (Angptl8)

  • 中文名稱：
  • 貨號：
    CSB-MP844436MO
  • 規(guī)格：
    ￥3960
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 別名：
    Betatrophin Lipasin Refeeding-induced fat and liver protein
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Full Length of Mature Protein
  • 來源：
    Mammalian cell
  • 分子量：
    23 kDa
  • 表達區(qū)域：
    16-198aa
  • 氨基酸序列
    VRPAPVAPLGGPEPAQYEELTLLFHGALQLGQALNGVYRATEARLTEAGHSLGLYDRALEFLGTEVRQGQDATQELRTSLSEIQVEEDALHLRAEATARSLGEVARAQQALRDTVRRLQVQLRGAWLGQAHQEFETLKARADKQSHLLWALTGHVQRQQREMAEQQQWLRQIQQRLHTAALPA-
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標(biāo)簽：
    N-terminal 10xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    18-28 business days
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Hormone that acts as a blood lipid regulator by regulating serum triglyceride levels. May be involved in the metabolic transition between fasting and refeeding: required to direct fatty acids to adipose tissue for storage in the fed state. According to a report, may act by promoting ANGPTL3 cleavage. According to another study, not required for cleavage of ANGPTL3.
  • 基因功能參考文獻：
    1. ANGPTL8 has a functional LPL inhibitory motif, but only inhibits LPL and increases plasma TG levels in mice in the presence of ANGPTL3 PMID: 28413163
    2. knock-down of Angptl8 in adipocytes suggests that the protein acts to inhibit intracellular lipolysis, analogous to its activity in the circulation. Depletion of Angptl8 results in an altered cellular phospholipid composition. The findings identify Angptl8 as a central insulin-regulated controller of adipocyte lipid metabolism. PMID: 28528274
    3. The high levels of ANGPTL8 found in fetal life together with its relationship with newborn adiposity and brown adipose tissue point to ANGPTL8 as a potential new player in the modulation of the thermogenic machinery during the fetal-neonatal transition. PMID: 27469268
    4. Obesity is associated with increased betatrophin suppression after an oral glucose load; this response appears to be driven by hyperglycemia. Data suggest that insulin or low glucose (as in hypoglycemia) increases betatrophin expression/secretion in adipocytes (3T3-L1 cells here); elevation of glucose levels blunts this effect. PMID: 27459526
    5. circulating betatrophin is increased in mice and humans with NAFLD and its expression was induced by endoplasmic reticulum stress in hepatocytes PMID: 27045862
    6. The expression of betatrophin is upregulated in the liver, white adipose tissue (WAT) and brown adipose tissue in obese hyperinsulinemic type 2 diabetes but decreased in WAT in hypoinsulinemic type 1 diabetes, suggesting its positive correlation with body weight and plasma insulin but not blood glucose. PMID: 27097546
    7. This model suggests a general mechanism by which TAG trafficking is coordinated by lipasin, Angptl3 and Angptl4 at different nutritional statuses. PMID: 26687026
    8. ANGPTL8 is a stress-response protein that down-regulates expression of ATGL. PMID: 26569053
    9. no substantial change in beta cell replication, mass or glucose homeostasis was observed following ANGPTL8 overexpression PMID: 25917759
    10. A monoclonal antibody against Angptl8, a feeding-induced hepatokine, lowers serum triglyceride levels when injected into mice. Angptl8 inhibits the activity of lipoprotein lipase in cardiac and skeletal muscle, but not in white adipose tissue. Therefore, Angptl8 acts, at least in part, in an endocrine manner, and its inhibition has therapeutical potential in treating hypertriglyceridemia. PMID: 26687026
    11. ANGPTL8 is not required for beta cell function or the compensatory beta cell growth response to insulin resistance, overexpression of ANGPTL8 does not increase beta cell area nor improve glycemic control, and ANGPTL8 regulates plasma triglyceride levels. PMID: 25417115
    12. Mice lacking ANGPTL8 (Betatrophin) manifest disrupted triglyceride metabolism without impaired glucose homeostasis. PMID: 24043787
    13. These results suggest Lipasin and some ANGPTLs play distinct physiological roles in mediating thermogenesis and energy homeostasis. PMID: 23261442
    14. Lipasin is a novel but atypical member of the angiopoietin-like protein family. PMID: 23261442
    15. Lipasin is thus a novel nutritionally-regulated liver-enriched factor that plays a role in lipid metabolism. PMID: 22809513
    16. RIFL is an important new regulator of lipid metabolism. PMID: 22569073

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    ANGPTL8 family
  • 組織特異性：
    Expressed in liver and fat. Enriched in white and brown adipose tissues.
  • 數(shù)據(jù)庫鏈接：