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  4. Recombinant Mouse Anoctamin-1 (Ano1), partial

Recombinant Mouse Anoctamin-1 (Ano1), partial

  • 中文名稱：
    Recombinant Mouse Anoctamin-1(Ano1) ,partial,Yeast
  • 貨號：
    CSB-YP804805MO
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Mouse Anoctamin-1(Ano1) ,partial,Yeast
  • 貨號：
    CSB-EP804805MO
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Mouse Anoctamin-1(Ano1) ,partial,Yeast
  • 貨號：
    CSB-EP804805MO-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Mouse Anoctamin-1(Ano1) ,partial,Yeast
  • 貨號：
    CSB-BP804805MO
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Mouse Anoctamin-1(Ano1) ,partial,Yeast
  • 貨號：
    CSB-MP804805MO
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Ano1
  • Uniprot No.：
  • 別名：
    Ano1; Tmem16aAnoctamin-1; Transmembrane protein 16A
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Calcium-activated chloride channel (CaCC) which plays an important role in transepithelial anion transport and smooth muscle contraction. Required for the normal functioning of the interstitial cells of Cajal (ICCs) which generate electrical pacemaker activity in gastrointestinal smooth muscles. Acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development.
  • 基因功能參考文獻：
    1. Bile acids stimulate Cl(-) secretion in biliary cells through activation of membrane TMEM16A channels in a process regulated by extracellular ATP and [Ca(2+) ]i . PMID: 29360145
    2. TMEM16A modulates arterial contractility, at least in part, indirectly via regulation of CACNA1C expression. PMID: 29438598
    3. the glutamic acids (E) at 143 and 705 residues in ANO1 are critical for modulation of Ca2 and/or heat responses. PMID: 29335069
    4. anoctamin-1 is involved in psychiatric behavior because of its role in the regulation of synaptic transmission in presynaptic terminals PMID: 29928889
    5. de novo atomic structures of the transmembrane domains of mouse TMEM16A in nanodiscs and in lauryl maltose neopentyl glycol as determined by single-particle electron cryo-microscopy PMID: 29236684
    6. cryo-EM structures of mouse TMEM16A at high resolution in the presence and absence of Ca(2+) PMID: 29236691
    7. The protein shows a similar organization to nhTMEM16, except for changes at the site of catalysis. There, the conformation of transmembrane helices constituting a membrane-spanning furrow that provides a path for lipids in scramblases has changed to form an enclosed aqueous pore that is largely shielded from the membrane. PMID: 28561733
    8. ANO1 immunoreactivity was observed in the presynaptic terminals of various retinal neurons, including photoreceptors PMID: 23840801
    9. In this work we demonstrate that increasing the extracellular proton concentration from 10-10 to 10-5.5 m enabled TMEM16A activation. Furthermore, we show that this effect is voltage independent, caused by changes in the apparent open probability of the channel, and due to protonation of extracellular residue E623 PMID: 27859335
    10. The present study shows that ANO1 and CavL play a central role in the generation of slow waves, phasic contractions and tone in the internal anal sphincter and that this pathway can occur in the absence of stretch PMID: 28054347
    11. findings demonstrate that Ca2+ influx via store-operated CRAC channels is essential for CaCC activation, chloride secretion, and sweat production in humans and mice. PMID: 27721237
    12. We knocked out Ano1 to varying degrees in interstitial cells of Cajal (ICC) of adult mice. Partial knockout of Ano1 shortened the widths of electrical slow waves and Ca(2+) transients in myenteric ICC but Ca(2+) transient synchronicity was preserved. Near-complete knockout was necessary for transient desynchronization and loss of slow waves, indicating a large functional reserve of Ano1 in ICC. PMID: 27979828
    13. Phosphorylation levels of P38 and JNK in siRNA-TMEM16A group were lower than that of the Model group. Thus, TMEM16A is one of the critical components of a signal transduction pathway that links renal injury to podocyte apoptosis in DN. PMID: 28392397
    14. To determine the role of ANO1 in physiological functions. PMID: 27413167
    15. ANO1/TMEM16A is a significant pathway in pancreatic acinar cells for HCO3 (-) secretion into the lumen. PMID: 27510033
    16. TMEM16A is a positive regulator of endothelial reactive oxygen species generation via Nox2-containing NADPH oxidase, which induces endothelial dysfunction and hypertension. PMID: 28320851
    17. Data indicate that anoctamin channel proteins ANO1 and ANO2 are expressed in the cerebellum. PMID: 26558388
    18. Ano1 may play a role in the pathophysiological processes during ischaemia in heart, and therefore, Ano1 might be a potential therapeutic target to prevent ischaemic damage PMID: 24758567
    19. Thegenetic ablation of TMEM16A did not seem to affect the maturation of olfactory sensory neurons and their ciliary layer. PMID: 26067252
    20. Suggest Ca(2+)-dependent push-pull conformational change as a key electromechanical movement for gating the ANO1 channel. PMID: 25231974
    21. These results indicate ANO1 channels are involved with TRPV1 actions in sensory neurons and inhibition of ANO1 could be a novel means of inducing analgesia. PMID: 26364309
    22. Basolateral Ano1 supports CCH-induced Ca2+ signaling and secretion via luminal CFTR. PMID: 24974903
    23. By inhibiting TRPM7 and ANO1 via opioid receptor signaling pathways. PMID: 25967872
    24. TMEM16A is an essential component of Ca(2+)-activated Cl(-) currents in mouse vomeronasal sensory neurons. PMID: 25779870
    25. conclude that ANO1 encodes Ca(2+)-activated chloride (Cl(-)) channel and plays a significant role in the phase 1 repolarization of action potentials PMID: 24962810
    26. TMEM16A and myocardin form a positive feedback loop that is disrupted by KLF5 during Ang II-induced vascular remodeling. PMID: 26077572
    27. ANO1 is thus mainly localized at presynaptic terminals in various brain regions, specifically in two auditory brainstem nuclei. PMID: 24853671
    28. These results indicate that TRPV1-ANO1 interaction is a significant pain-enhancing mechanism in the peripheral nervous system. PMID: 25848051
    29. WT TMEM16A but not mutants with alanine substitution at four basic residues exhibited a clear decline in the preference for larger anions as intracellular calcium was increased. PMID: 25733897
    30. TMEM16a is a Ca(2+) -activated Cl(-) channel expressed in the collecting ducts. PMID: 24913262
    31. results using acinar-specific Tmem16A(-/-) mice identify Tmem16A as the Cl(-) channel essential for muscarinic, Ca(2+)-dependent fluid secretion in adult mouse salivary glands PMID: 25646474
    32. Null deletion of TMEM16A reduced the Ca(2+)-activated Cl(-) currents and action potential firing in spiral ganglion neurons. PMID: 25675481
    33. Ano1 has a role in proximal tubular proton secretion and protein reabsorption. PMID: 24476694
    34. Calcium-activated chloride secretion by anoctamin 1 appears to be a crucial component of renal cyst growth. PMID: 24152967
    35. Selective antagonism of ANO1 promotes relaxation of spontaneous myometrial contractions. It inhibits both agonist-induced and spontaneous transient inward currents and abolishes G-protein coupled receptor mediated elevations in intracellular calcium. PMID: 24928056
    36. The Ca(2+)-activated Cl ((-)) channel anoctamin-1 (ANO1/TMEM16A) is located in the primary cilium and that blocking its channel function pharmacologically or knocking it down with short hairpin RNA interferes with ciliogenesis. PMID: 24694595
    37. Data suggest different functional roles for TMEM16A and TMEM16B in the developing as well as in the postnatal olfactory epithelium PMID: 24318978
    38. TMEM16A is a major component of Ca(2+)-activated Cl(-) channel (CaCC) conductance in murine portal vein smooth muscle cells (mPVSMCs). PMID: 24770592
    39. Anoctamin (Ano)1 activation by the divalent calcium (Ca)2+ channel occurs by direct binding of Ca2+ to Ano1. The activation of Ano1 by Ca2+ is not mediated by calmodulin (CaM). PMID: 24420770
    40. ANO1 is also capable of augmenting the excitability of dorsal-root ganglion neurons under inflammatory or neuropathic conditions. PMID: 24450308
    41. Ano1 is expressed in ovarian granulosa cells and downregulates estrogen production, possibly through the MEK-ERK signaling cascade. PMID: 24823391
    42. findings show TMEM16A and TMEM16F have an important function in cell migration-TMEM16A in directional migration, TMEM16F in determination of speed of migration; conclude that TMEM16A and TMEM16F channels have a distinct impact on steering and motor mechanisms of migrating ELA cells PMID: 23832500
    43. TMEM16A and -16F use a similar mechanism for sorting to plasma membrane and protein stabilization, but their functional domains significantly differ PMID: 24478309
    44. TMEM16A plays a general role in arteriolar and capillary blood flow and may be involved in reducing hypertension PMID: 24401273
    45. In conclusion, alternatively spliced variants of TMEM16A abc and acd in mPVSMCs are two major molecular entities of CaCC and may form hetero-/homo-dimers to be functional as CaCC in the regulation of membrane excitability and contractility in mPVSMCs. PMID: 24321548
    46. ANO1 decreased activity and expression is associated with abnormal cystic fibrosis airway epithelial repair. PMID: 24080196
    47. The putative pore-loop of TMEM16A and TMEM16B channels affects channel density in cell membranes. PMID: 23613533
    48. These data provide evidence for a functional role of TMEM16A in the vasoconstrictor responses of murine thoracic aorta and mesenteric artery as well as human resistance vessels. PMID: 22946562
    49. 16A Ca(2+)-activated Cl-channels are inferred to be involved in airway hyperresponsiveness. PMID: 23239156
    50. HCO3- permeability of ANO1 can be dynamically modulated by Ca2+/calmodulin. PMID: 23248295

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  • 亞細胞定位：
    Cell membrane; Multi-pass membrane protein. Cytoplasm.
  • 蛋白家族：
    Anoctamin family
  • 組織特異性：
    Highly expressed in pulmonary bronchiole epithelial cells, pancreatic and submandibular gland acinar cells, kidney proximal tubule, all retinal cell layers, most sensory cells of dorsal root ganglia, Leydig cells and spermatocytes (at protein level). Expr
  • 數(shù)據(jù)庫鏈接：