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  4. Recombinant Mouse Breast cancer type 2 susceptibility protein homolog (Brca2), partial

Recombinant Mouse Breast cancer type 2 susceptibility protein homolog (Brca2), partial

  • 中文名稱：
    Recombinant Mouse Breast cancer type 2 susceptibility protein homolog(Brca2) ,partial,Yeast
  • 貨號：
    CSB-YP308755MO
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Mouse Breast cancer type 2 susceptibility protein homolog(Brca2) ,partial,Yeast
  • 貨號：
    CSB-EP308755MO
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Mouse Breast cancer type 2 susceptibility protein homolog(Brca2) ,partial,Yeast
  • 貨號：
    CSB-EP308755MO-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Mouse Breast cancer type 2 susceptibility protein homolog(Brca2) ,partial,Yeast
  • 貨號：
    CSB-BP308755MO
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Mouse Breast cancer type 2 susceptibility protein homolog(Brca2) ,partial,Yeast
  • 貨號：
    CSB-MP308755MO
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    Brca2; Fancd1; Breast cancer type 2 susceptibility protein homolog; Fanconi anemia group D1 protein homolog
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA). Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. Part of a PALB2-scaffolded HR complex containing RAD51C and which is thought to play a role in DNA repair by HR. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks; together with PALB2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity. In concert with NPM1, regulates centrosome duplication. Interacts with the TREX-2 complex (transcription and export complex 2) subunits PCID2 and SEM1, and is required to prevent R-loop-associated DNA damage and thus transcription-associated genomic instability, independently of its known role in homologous recombination.
  • 基因功能參考文獻(xiàn)：
    1. BRCA2-proficient and deficient cells are radiosensitised by HT, indicating that HT does not exclusively act by inhibition of HR. PMID: 28540821
    2. Results suggest that the greater reliance on homology-directed repair (HDR) in the proliferating mammary gland, rather than a specific dependence on breast cancer 2 protein (BRCA2), may increase its susceptibility to tumorigenesis incurred by BRCA2 mutation. PMID: 27779185
    3. The functional consequence of Parp1 heterozygosity on BRCA2 loss is demonstrated by a significant increase in tumorigenesis in Brca2 knockout mice. PMID: 27498558
    4. we generated a Brca2 knock-in mouse model lacking exons 4-7 and demonstrated that these exons are dispensable for viability as well as tumor-free survival. This study provides the first in vivo evidence of the functional significance of a minor transcript of BRCA2 that can play a major role in the survival of humans who are homozygous for a clearly pathogenic mutation. PMID: 26920070
    5. we describe a genetic approach to examine the functional significance of the interaction between BRCA2 and PALB2 by generating a knock-in mouse model of Brca2 carrying a single amino acid change (Gly25Arg, Brca2G25R) that disrupts this interaction. In addition, we have combined Brca2G25R homozygosity as well as hemizygosity with Palb2 and Trp53 heterozygosity . PMID: 27490902
    6. Merit40 mutation exacerbated ICL-induced chromosome instability in the context of concomitant Brca2 deficiency but not in conjunction with Fancd2 mutation. PMID: 26338419
    7. Heterozygous and homozygous Brca2 mutation may lead to dysfunction in T cell populations. PMID: 25666348
    8. BRCA2 exon 27 domain maintains chromosomal integrity at both stalled and collapsed replication forks consistent with involvement in both replication fork maintenance and double strand break repair. PMID: 25773776
    9. we use a genetically engineered mouse model of BRCA2-associated hereditary breast cancer to study drug resistance to several types of chemotherapy and PARP inhibition. PMID: 25511378
    10. BRCA2-mediated sequestration of nuclear RAD51 serves to prevent inappropriate DNA interactions. PMID: 25488918
    11. BRCA2 directly represses the expression of IFN-related genes PMID: 25043256
    12. the models reveal novel aspects of cancer evolution in carriers of germline BRCA2 mutations, provide new insights into the tumour suppressive role of BRCA2 PMID: 24268522
    13. genetic stability, and hematopoietic differentiation potential of gene-corrected Brca2(Delta) (27/) (Delta) (27) iPSCs, achievements and limitations in the application of current reprogramming approaches in hematopoietic stem cell therapy are also discussed. PMID: 24420904
    14. Results suggest that cellular levels of Brca2 and Rad51 are mutually dependent on each other, and that low levels of these proteins provide selective pressure for reduction of p53, which permits cell growth PMID: 24210700
    15. BRCA2 accumulates DNA damage, which triggers checkpoint signalling and ARF activation PMID: 24162189
    16. data showed that silencing of BRCA2 promoted cell proliferation, migration and invasion in vitro; data reported here demonstrate that BRCA2 may be a promising therapeutic targets for pancreatic ductal adenocarcinoma progression PMID: 22934697
    17. BRCA2 is required for telomere homeostasis and may be particularly important for the replication of G-rich telomeric lagging strands. PMID: 22187435
    18. Loss of Brca2 function predisposes the exocrine pancreas to profound DNA damage, and the frequency of invasive neoplasia is accentuated by the concomitant deregulation of p53 PMID: 21455033
    19. FANCD1/BRCA2 played notably important roles in the repair of TMZ-induced DNA damage. PMID: 21573016
    20. Data suggest that the growth-suppressive effect of CHK1 inhibition in BRCA2-mutant tumors can be opposed by concurrent KRAS activation and TP53 mutations. PMID: 21289082
    21. Trp53 signaling must be modified before inactivation of the Brca2 wild-type allele, irrespective of Kras status, for Brca2-deficient cells to form pancreatic tumors PMID: 21199651
    22. BRCA2 associates with telomeres during the S and G2 phases of the cell cycle and facilitates the loading of RAD51 onto telomeres. PMID: 21076401
    23. Germline heterozygosity for a pathogenic Brca2 truncation suffices to promote pancreatic ductal adenocarcinomas driven by Kras(G12D), irrespective of Trp53 status. PMID: 21056012
    24. Simultaneous deletion of Brca2 and the tumour suppressor Trp53 in prostate epithelia gave rise to focal hyperplasia and atypical cells PMID: 20585617
    25. Loss of Brca2 increases in vivo somatic mutation acquisition and synergizes with ionizing radiation exposure. PMID: 11850397
    26. Genomic organization and expression of the mouse Brca2 gene PMID: 12140683
    27. crystal structure of a COOH terminal BRCA2 domain bound to DSS1; demonstrate that this BRCA2 domain binds single-stranded DNA and show that BRCA2 stimulates RAD51-mediated recombination in vitro PMID: 12228710
    28. The extreme carboxy-terminal region encoded by exon 27 is vital for BRCA2 function in mice, perhaps because it is required for a fully functional interaction between BRCA2 and RAD51, though it is less severe than truncations that delete some BRC motifs. PMID: 12619154
    29. BRCA2 has a role in modulating M phase progression PMID: 12815053
    30. interplay between Brca2 and mitotic checkpoint has been suggested in the maintenance of genetic fidelity PMID: 14646599
    31. BRCA2-deficient spermatocytes fail to progress beyond the early prophase I stage of meiosis. PMID: 14660434
    32. Brca2 knockout mouse is less susceptible to chemically indeced tumorigenesis. PMID: 14981540
    33. results show that BRCA2 deficiency impairs the completion of cell division by cytokinesis PMID: 15375219
    34. Brca2 plays an important role in the response to DNA damage in the small intestine. PMID: 15735671
    35. In the small intestine, deletion of cells lacking Brca2 is necessary to avoid the development of potentially tumorigenic clones in this tissue. PMID: 16042582
    36. Homozygous germ-line mutation in exon 27 of Brca2 disrupts the Fancd2-Brca2 pathway in the homologous recombination-mediated DNA interstrand cross-links' repair, but does not affect meiosis. PMID: 16127665
    37. These findings include extensive in vivo data demonstrating that germline Brca2 and p53 mutations cooperatively affect animal survivals, tumor susceptibilities, and tumor onsets. PMID: 16546942
    38. These results identify a novel role for FoxM1 in the transcriptional response during DNA damage/checkpoint signaling and show a novel mechanism by which Chk2 protein regulates expression of DNA repair enzymes. PMID: 17101782
    39. Brca2 has roles in DNA repair and recombination PMID: 17336596
    40. BRCA2 is required for neurogenesis and suppression of medulloblastoma. PMID: 17476307
    41. Loss of Rad51 function as a critical underlying factor in the homologous recombination defect in the Brca2-depleted cells. PMID: 18992372
    42. Article describes the involvement of BRCA2 in O6-alkylguanine DNA alkyltransferase (AGT)-mediated repair of O6-methylguanine adducts. PMID: 19047179
    43. Murine Brca2 shares multiple properties with human BRCA2 including its regulation during the cell cycle, localization to nuclear foci, and interaction with Brca1 and Rad51. PMID: 11477095

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  • 亞細(xì)胞定位：
    Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
  • 組織特異性：
    Widely expressed. Highest expression in cerebellum, testis, ileum, appendix, epididymis, ovary and mammary gland. No expression in lung.
  • 數(shù)據(jù)庫鏈接：