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Recombinant Mouse CD5 antigen-like (Cd5l)

  • 中文名稱(chēng)：
    Recombinant Mouse CD5 antigen-like (Cd5l)
  • 貨號(hào)：
    CSB-EP874099MO
  • 規(guī)格：
    ￥1536
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 85% as determined by SDS-PAGE.
  • 生物活性：
    Not Test
  • 基因名：
    Cd5l
  • Uniprot No.：
  • 別名：
    Apoptosis inhibitor expressed by macrophages;mAIM;Apoptosis inhibitory 6;SP-alpha
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長(zhǎng)度：
    Full Length of Mature Protein
  • 來(lái)源：
    E.coli
  • 分子量：
    43.6 kDa
  • 表達(dá)區(qū)域：
    22-352aa
  • 氨基酸序列
    ESPTKVQLVGGAHRCEGRVEVEHNGQWGTVCDDGWDRRDVAVVCRELNCGAVIQTPRGASYQPPASEQRVLIQGVDCNGTEDTLAQCELNYDVFDCSHEEDAGAQCENPDSDLLFIPEDVRLVDGPGHCQGRVEVLHQSQWSTVCKAGWNLQVSKVVCRQLGCGRALLTYGSCNKNTQGKGPIWMGKMSCSGQEANLRSCLLSRLENNCTHGEDTWMECEDPFELKLVGGDTPCSGRLEVLHKGSWGSVCDDNWGEKEDQVVCKQLGCGKSLHPSPKTRKIYGPGAGRIWLDDVNCSGKEQSLEFCRHRLWGYHDCTHKEDVEVICTDFDV
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標(biāo)簽：
    C-terminal 6xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Secreted protein that acts as a key regulator of lipid synthesis: mainly expressed by macrophages in lymphoid and inflamed tissues and regulates mechanisms in inflammatory responses, such as infection or atherosclerosis. Able to inhibit lipid droplet size in adipocytes. Following incorporation into mature adipocytes via CD36-mediated endocytosis, associates with cytosolic FASN, inhibiting fatty acid synthase activity and leading to lipolysis, the degradation of triacylglycerols into glycerol and free fatty acids (FFA). CD5L-induced lipolysis occurs with progression of obesity: participates in obesity-associated inflammation following recruitment of inflammatory macrophages into adipose tissues, a cause of insulin resistance and obesity-related metabolic disease. Regulation of intracellular lipids mediated by CD5L has a direct effect on transcription regulation mediated by nuclear receptors ROR-gamma (RORC). Acts as a key regulator of metabolic switch in T-helper Th17 cells. Regulates the expression of pro-inflammatory genes in Th17 cells by altering the lipid content and limiting synthesis of cholesterol ligand of RORC, the master transcription factor of Th17-cell differentiation. CD5L is mainly present in non-pathogenic Th17 cells, where it decreases the content of polyunsaturated fatty acyls (PUFA), affecting two metabolic proteins MSMO1 and CYP51A1, which synthesize ligands of RORC, limiting RORC activity and expression of pro-inflammatory genes. Participates in obesity-associated autoimmunity via its association with IgM, interfering with the binding of IgM to Fcalpha/mu receptor and enhancing the development of long-lived plasma cells that produce high-affinity IgG autoantibodies. Also acts as an inhibitor of apoptosis in macrophages: promotes macrophage survival from the apoptotic effects of oxidized lipids in case of atherosclerosis. Involved in early response to microbial infection against various pathogens by acting as a pattern recognition receptor and by promoting autophagy.
  • 基因功能參考文獻(xiàn)：
    1. AIM/CD5L-deficient mice demonstrated a smaller infarct size and less of an inflammatory response via inflammatory pathways after myocardial infarction; these data provide the first evidence that AIM/CD5L plays an important role in the inflammatory responses after myocardial injury PMID: 27045005
    2. AIM enhances intratubular debris clearance and ameliorates acute kidney injury via interaction with KIM-1. PMID: 26726878
    3. CD5L as a critical regulator that distinguishes Th17 functional states and identified T cell lipid metabolism as an integral component of the pathways regulating Th17 cell pathogenicity. PMID: 26607793
    4. plays a general homeostatic role by supporting innate immunity during pathogen aggression PMID: 24583716
    5. the assembled p40-CD5L heterodimer does not recapitulate the biological activity of IL-12. PMID: 24821971
    6. AIM contributes to key macrophage responses to M. tuberculosis PMID: 24223991
    7. AIM-IgM association plays a critical role in the obesity-associated autoimmune process. PMID: 23562157
    8. SREBP-1a specifically activates the Api6 gene through a binding site in its proximal promoter, thus establishing the Api6 gene as a newly identified SREBP-1a target gene PMID: 22493063
    9. N-glycosylation plays important roles in the secretion and lipolytic function of AIM PMID: 23236605
    10. AIM induces lipolysis in a manner distinct from that of hormone-dependent lipolysis, without activation or augmentation of lipases. PMID: 22579686
    11. Apoptosis inhibitor of macrophage (AIM) is required for obesity-associated recruitment of inflammatory macrophages into adipose tissue. PMID: 21730133
    12. AIM binds to cytosolic FAS protein at various regions responsible for elongation of fatty acids, release of synthesized palmitate, and stabilization of FAS dimerization. PMID: 20519120
    13. The protein inhibits apoptosis of T cells and NKT cells in Corynebacterium-induced granuloma formation in mice. PMID: 12598318
    14. Api6 production facilitates macrophage survival within atherosclerotic lesions and that loss of Api6 decreases early lesion development by increasing macrophage apoptosis PMID: 16054063
    15. Bacterial lipoteichoic acid might affect the pathogenesis of epithelial inflammation followed by fibrosis in systemic multifocal epithelial inflammations in chronic colitis-harboring TCR alpha(-/-) x AIM(-/-) mice PMID: 17804558

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  • 亞細(xì)胞定位：
    Secreted. Cytoplasm.
  • 組織特異性：
    Specifically expressed in tissue macrophages. Expressed in thymus, liver, spleen and lymph nodes. Present in Th17 cells; mainly present in non-pathogenic Th17 cells.
  • 數(shù)據(jù)庫(kù)鏈接：