E. coli biotin ligase
(BirA) is highly specific in covalently attaching biotin to the 15 amino
acid AviTag peptide. This recombinant protein was biotinylated in vivo
by AviTag-BirA technology, which method is BriA catalyzes amide linkage
between the biotin and the specific lysine of the AviTag.
The tag type will be
determined during production process. If you have specified tag
type, please tell us and we will develop the specified tag
preferentially.
產(chǎn)品提供形式:
Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶:
We recommend that this vial be briefly centrifuged prior
to opening to bring the contents to the bottom. Please reconstitute
protein in deionized sterile water to a concentration of 0.1-1.0
mg/mL.We recommend to add 5-50% of glycerol (final concentration) and
aliquot for long-term storage at -20℃/-80℃. Our default final
concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for
mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期:
The shelf life is related to many factors, storage state,
buffer ingredients, storage temperature and the stability of the protein
itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The
shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期:
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our
proteins are default shipped with normal blue ice packs, if you
request to ship with dry ice, please communicate with us in advance
and extra fees will be charged.
注意事項(xiàng):
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.
基因功能參考文獻(xiàn):
FtMt overexpressing mice have no significant defects and the overexpression of FtMt does not affect the regulation of iron metabolism significantly in transgenic mice. PMID: 28703745
Ferritin is considered the major iron storage protein which maintains a large iron core in its cavity and has ferroxidase activity. (Review) PMID: 28349628
the absence of Mitochondrial ferritin, which is highly expressed in the heart, increases the sensitivity of mitochondria to cardiac injury via oxidative stress. PMID: 27853170
As previously reported, homozygous loss of the Fth allele on a wild-type Ftl background was embryonic lethal. expression of the H subunit can rescue the loss of the L subunit and that H ferritin homopolymers have the capacity to sequester iron in vivo PMID: 25629408
provide a new mechanism for selective autophagy of ferritin and reveal a previously unappreciated role for autophagy and NCOA4 in the control of iron homeostasis in vivo PMID: 25327288
Our data show that ferritin H is required for B and T cell survival by actively reducing the labile iron pool. PMID: 24586648
Ferritin H deficiency in most cells of the forebrain including cells of the choroid plexus caused accumulation of cerebrospinal fluid in the lateral ventricles and the subarachnoid space PMID: 24662374
Mycobacterium avium infection induces H-ferritin expression in mouse primary macrophages by activating Toll-like receptor 2. PMID: 24349383
3-Hydroxybutyrate dehydrogenase-2 and ferritin-H synergistically regulate intracellular iron. PMID: 24673886
Fth plays a critical protective role during acute kidney injury. PMID: 24018561
FtH provides metabolic adaptation to tissue Fe overload, conferring tolerance to malaria PMID: 23159058
The delivery of iron to oligodendrocytes via H-ferritin is a biologically relevant delivery system, given that ferritin--not transferrin--receptors can be demonstrated on oligodendrocytes in vivo. PMID: 21446040
HFt taken up by TIM-2 positive cells transited through the endosome and eventually entered a lysosomal compartment, distinguishing the HFt pathway from that of transferrin PMID: 21886823
Mitochondrial ferritin is found in all regions of the brain in C57/BL6 mice; the role of mitochondrial ferritin can be expected to contribute to our knowledge of mitochondrial dysfunction and neurodegenerative disease. PMID: 20722075
Loss of one allele of H ferritin appears to increase light-induced degeneration. PMID: 20881284
Hx plays an important role in controlling iron distribution within brain, thus suggesting its involvement in iron-related neurodegenerative diseases. PMID: 19120692
FTH expression is mediated by Nrf2 in response to xenobiotics and cancer chemopreventive dithiolethiones PMID: 12435735
Ferritin heavy chain 1 necessary for tumor necrosis factor alpha-induced apoptosis in L929 cells. PMID: 16024802
excessive free iron and the resultant enhanced oxidative stress caused by downregulation of FHC lead to cardiomyocyte death PMID: 18992754