E. coli biotin ligase
(BirA) is highly specific in covalently attaching biotin to the 15 amino
acid AviTag peptide. This recombinant protein was biotinylated in vivo
by AviTag-BirA technology, which method is BriA catalyzes amide linkage
between the biotin and the specific lysine of the AviTag.
The tag type will be
determined during production process. If you have specified tag
type, please tell us and we will develop the specified tag
preferentially.
產品提供形式:
Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶:
We recommend that this vial be briefly centrifuged prior
to opening to bring the contents to the bottom. Please reconstitute
protein in deionized sterile water to a concentration of 0.1-1.0
mg/mL.We recommend to add 5-50% of glycerol (final concentration) and
aliquot for long-term storage at -20℃/-80℃. Our default final
concentration of glycerol is 50%. Customers could use it as reference.
儲存條件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for
mutiple use. Avoid repeated freeze-thaw cycles.
保質期:
The shelf life is related to many factors, storage state,
buffer ingredients, storage temperature and the stability of the protein
itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The
shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期:
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our
proteins are default shipped with normal blue ice packs, if you
request to ship with dry ice, please communicate with us in advance
and extra fees will be charged.
注意事項:
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Plays a critical role in the sodium-dependent reabsorption of bile acids from the lumen of the small intestine. Plays a key role in cholesterol metabolism.
基因功能參考文獻:
data support the concept that under physiological conditions microbiota stimulate Gata4, which suppresses Asbt expression PMID: 26022694
In Asbt-deficient mice, the lack of bile acid uptake in the distal small intestine, which we recently documented using novel in vivo imaging results in more than 10-fold increased fecal bile acids PMID: 26210740
ileal ASBT protein is degraded by a ubiquitin-dependent pathway in response to enterobacteria-associated bile acids PMID: 23872411
Ileal Fibroblast Growth Factor-15 (FGF15) expression was significantly reduced in Asbt(-/-)apoE(-/-) mice and was inversely correlated with expression of hepatic cholesterol 7-hydroxylase (Cyp7a1). PMID: 23880190
The functional importance of proline and uncharged polar residues in the highly conserved region of mouse Slc10a2 was determined. PMID: 23374508
Enterobacteria-mediated bile acid biotransformation modulates intestinal bile acid transport via downregulation of SLC10A2 expression. PMID: 20884752
These data confirm that bile acids and upregulation of ASBT play a crucial role in NEC pathogenesis and suggest that inhibition of ASBT could be utilized as a therapeutic modality against this disease. PMID: 20616306
Slc10a2 is essential for efficient intestinal absorption of bile acids PMID: 12819193
ASBT-mediated bile salt uptake in the distal ileum might also modulate intestinal fluid secretion PMID: 16037545
The lithogenic diet induced biliary hyperplasia and reduced bile salt transporter Slc10a2 expression in C57L mice. PMID: 18717763
Demonstrate a posttranscriptional modulation of ileal ASBT function and membrane expression by phorbol ester via a PKCzeta-dependent pathway. PMID: 19571234
Highly conserved region contains residues involved in the substrate interaction, function, and cellular localization of mSlc10a2. PMID: 19584562
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亞細胞定位:
Membrane; Multi-pass membrane protein.
蛋白家族:
Bile acid:sodium symporter (BASS) (TC 2.A.28) family