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  4. Recombinant Mouse Lysine-specific histone demethylase 1A (Kdm1a)

Recombinant Mouse Lysine-specific histone demethylase 1A (Kdm1a)

  • 中文名稱：
    小鼠Kdm1a重組蛋白
  • 貨號(hào)：
    CSB-YP751166MO
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Kdm1a重組蛋白
  • 貨號(hào)：
    CSB-EP751166MO
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Kdm1a重組蛋白
  • 貨號(hào)：
    CSB-EP751166MO-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Kdm1a重組蛋白
  • 貨號(hào)：
    CSB-BP751166MO
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Kdm1a重組蛋白
  • 貨號(hào)：
    CSB-MP751166MO
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    Kdm1a; Aof2; Kiaa0601; Lsd1; Lysine-specific histone demethylase 1A; EC 1.-.-.-; BRAF35-HDAC complex protein BHC110; Flavin-containing amine oxidase domain-containing protein 2
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長(zhǎng)度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Histone demethylase that can demethylate both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Effector of SNAI1-mediated transcription repression of E-cadherin/CDH1, CDN7 and KRT8. Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7.
  • 基因功能參考文獻(xiàn)：
    1. These results indicate that LSD1 activators or miRNA antagonists could serve as a therapeutic approach for life-threatening septic shock characterized by dysfunction of hematopoietic stem cells. PMID: 29263096
    2. MiR-137-3p was upregulated in Bv-injured DRGNs. MiR-137-3p downregulation rescued Bv-induced DRGN apoptosis and neurite retraction. MiR-137-3p and its downstream target LSD1 are inversely associated to regulate anesthetics-induced neurotoxicity in DRGN. PMID: 28899795
    3. contrary to its role in enhancing DNA virus replication, LSD1 limits RNA virus replication by demethylating and activating IFITM3 which is a host restriction factor for many RNA viruses PMID: 29281729
    4. Data (including data from studies in transgenic/knockout mice) suggest that Kdm1a-mediated attenuation of Srebf1 transcriptional activities functions as underlying mechanism for suppression of de novo lipogenesis by oxidative stress in white adipose tissue. [Kdm1a = lysine (K)-specific demethylase-1A; Srebf1 = sterol-regulatory element-binding transcription factor-1] PMID: 29618580
    5. This study demonstrated that LSD1 expression during Olfactory Epithelium neuronal maturation. PMID: 28597915
    6. LSD1 having a role in silencing additional odorant receptor (OR) alleles, as opposed to being required for the activation of OR alleles, within theolfactory-placode-derived cell line cellular context PMID: 28414096
    7. results identify the LSD1/NuRD complex as a previously unrecognized modulator for Pax2-mediated neuronal differentiation in the inner ear PMID: 29370269
    8. Lsd1 regulates skeletal muscle regeneration and directs the fate of satellite cells into myocytes while preventing brown adipocyte differentiation of satellite cells via repressing expression of the novel pro-adipogenic transcription factor Glis1. PMID: 29371665
    9. LSD1 is continuously required to prevent neurodegeneration. Loss of LSD1 in adult mice leads to paralysis and neurodegeneration in the hippocampus and cortex. PMID: 28993646
    10. LSD1 consolidates into a single dominant compartment at the edges of chromocenters within nuclei of early post-mitotic cells of the mouse olfactory epithelium. LSD1 complexes with CoREST in early G1 of an immortalized olfactory cell line. PMID: 26947098
    11. LSD1 is required for the timely expression of MyoD in limb buds. PMID: 28228264
    12. Lsd1 is a key regulator of gene expression and metabolic function in brown adipose tissue. PMID: 27760309
    13. The Demethylase Activity of LSD1 Is Required for Brown Adipogenesis. PMID: 27642069
    14. through interaction with Zfp516, LSD1 is recruited to UCP1 and other brown adipose tissue-enriched genes. PMID: 27264172
    15. Study propose that KDM1A is a key regulator of spermatogenesis and germ cell maintenance in the mouse. PMID: 28498828
    16. This study demonstrates that the FGF19-SHP-LSD1 axis maintains homeostasis by suppressing unnecessary autophagic breakdown of cellular components, including lipids, under nutrient-rich postprandial conditions. PMID: 28446510
    17. This analysis identified that LSD1 is the only lysine demethylase required for myogenic differentiation and that KDM3B, KDM6A, and KDM8 are the candidate lysine demethylases required for osteoblast differentiation. PMID: 27732873
    18. Class I KDACs facilitate Dex-induced transcriptional repression by suppressing LSD1 complex activity at selected target gene promoters PMID: 27645313
    19. The study describes an interaction network of STAT5a/LSD1/HDAC3 and a dual function of LSD1/HDAC3 on STAT5a-dependent transcription, defined by protein-protein interactions, genomic binding localization/affinity and motifs. PMID: 27651463
    20. that nuclear GSK3beta- and USP22-mediated KDM1A stabilization is essential for glioblastoma tumorigenesis PMID: 27501329
    21. MALDI-TOF mass spectrometry indicates that hydrogen peroxide blocks labeling of cysteine 600, which we propose forms an intramolecular disulfide with cysteine 618 to negatively regulate the catalytic activity of KDM1A PMID: 27634040
    22. lysine-specific demethylase 1 LSD1 represses HSD11B1 (hydroxysteroid 11-beta-dehydrogenase isozyme 1), a key glucocorticoid-activating enzyme, independently from PRDM16 PMID: 27566776
    23. RIP140 protects LSD1's catalytic domain and antagonizes its Jade-2-mediated ubiquitination and degradation. PMID: 26372689
    24. Loss of maternal LSD1/KDM1A in mice results in embryonic arrest at the 1-2 cell stage, with arrested embryos failing to undergo the maternal-to-zygotic transition. PMID: 26814574
    25. KDM1A plays critical roles in establishing the correct epigenetic landscape of the zygote upon fertilization, in preserving genome integrity and in initiating new patterns of genome expression that drive early mouse development. PMID: 26836306
    26. Data show that CCAAT-enhancer-binding protein-alpha (C/EBPalpha) directly regulates Kruppel-like factor 4 (Klf4) expression and increasing the levels of histone demethylase Lsd1 and transcription factor Brd4 in B cell. PMID: 26974661
    27. Deletion or reduction of isoform neuro LSD1 translates into decreased levels of activating histone marks at egr1 and c-fos promoters, dampening their psychosocial stress-induced transcription and resulting in low anxiety-like behavior. PMID: 26976584
    28. Rcor2, a co-repressor of LSD1, controls neurogenesis in the developing mouse brain. PMID: 26795843
    29. GFI1 proteins recruit the chromatin-modifying protein LSD1, a member of the CoREST repressive complex, to epigenetically silence the endothelial program in haemogenic endothelium and allow the emergence of blood cells PMID: 26619147
    30. LSD1 is essential for oocyte meiotic progression by upregulating CDC25B expression. PMID: 26626423
    31. This study described how neuronal activity controls the neurospecific splicing of the epigenetic co-repressor LSD1 in mammalian nervous system. PMID: 24735673
    32. LSD1 mediates erythroid differentiation, via epigenetic modification of the GATA-2 locus. PMID: 26124638
    33. Kdm1a is a master controller of gene transcription in spermatogonia and is required for SSC and progenitor maintenance and differentiation. PMID: 26243864
    34. KDM1A overexpression in one generation severely impaired development and survivability of offspring. PMID: 26449473
    35. Selective genetic ablation of LSD1n led to deficits in spatial learning and memory. PMID: 26214369
    36. High LSD1 expression is associated with sickle cell anemia. PMID: 26031919
    37. novel histone-modifying enzyme in the orchestrated regulation mediated by the farnesoid X receptor and small heterodimer partner that reduces hepatic bile acid levels and protects the liver against bile acid toxicity PMID: 25545350
    38. LSD1 has a role in herpesvirus infection, shedding, and recurrence by promoting epigenetic suppression of viral genomes PMID: 25473037
    39. The present study was designed to detect the expression of Histone H3 lysine 4 methylation and its demethylases LSD1, RBP2 and SMCX in 21-, 40- and 60-day-old mouse testes. PMID: 25255718
    40. Study suggests that a histone demethylase is functionally involved in the regulation of the malignant phenotype of medulloblastoma cells, and lays a foundation for future evaluation of KDM1A-inihibiting therapies in combating medulloblastoma PMID: 24252778
    41. As a result of differences in the components and targets of the Rest complex, its functional roles may differ in embryonic stem cells and epiblast stem cells. PMID: 24752154
    42. propose that Zscan4 may regulate gene transcription in mouse ES cells through interaction with LSD1 and CtBP2 PMID: 24594919
    43. MyT1 is a subunit of the neural cell type-specific LSD1 complex. PMID: 24828497
    44. In mature erythroid cells, a strong upsurge in Rcor3 and a sharp decline in Rcor1 levels counteract LSD1/Rcor1/2-mediated differentiation. PMID: 24843136
    45. Knockdown of kdm1a or its partner REST corepressor 2 (rcor2) in 3T3-L1 preadipocytes results in increased expression of inflammatory cytokines and chemokines by these cells. PMID: 23595969
    46. LSD1 is a key component of the molecular circadian oscillator, which plays a pivotal role in rhythmicity and phase resetting of the circadian clock PMID: 24582500
    47. Beta-catenin can recruit the H3K4me2/1 demethylase LSD1 to regulate the expression of the tumor suppressor Lefty1 in mouse embryonic stem cells. PMID: 23592333
    48. These results identify a previously unknown role for Lsd1 in heart development, perhaps partly through the control of E-cadherin phosphorylation. PMID: 23637775
    49. Study identifies the transient expression of histone demethylase LSD1 and the OR-dependent expression of adenylyl cyclase 3 as requirements for initiation and stabilization of OR expression. PMID: 23870122
    50. The function of CoRest in pyramidal cell neurogenesis requires the histone demethylase LSD1. PMID: 21878487

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  • 亞細(xì)胞定位：
    Nucleus.
  • 蛋白家族：
    Flavin monoamine oxidase family
  • 組織特異性：
    Ubiquitously expressed.
  • 數(shù)據(jù)庫(kù)鏈接：