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  4. Recombinant Mouse Methylcytosine dioxygenase TET3 (Tet3), partial

Recombinant Mouse Methylcytosine dioxygenase TET3 (Tet3), partial

  • 中文名稱：
    小鼠Tet3重組蛋白
  • 貨號：
    CSB-YP803850MO
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Tet3重組蛋白
  • 貨號：
    CSB-EP803850MO
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Tet3重組蛋白
  • 貨號：
    CSB-EP803850MO-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Tet3重組蛋白
  • 貨號：
    CSB-BP803850MO
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Tet3重組蛋白
  • 貨號：
    CSB-MP803850MO
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Tet3
  • Uniprot No.：
  • 別名：
    Tet3; Methylcytosine dioxygenase TET3; EC 1.14.11.n2
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in epigenetic chromatin reprogramming in the zygote following fertilization. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. Selectively binds to the promoter region of target genes and contributes to regulate the expression of numerous developmental genes. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in DNA demethylation in the paternal pronucleus by mediating conversion of 5mC into 5hmC, 5fC and 5caC. Does not mediate DNA demethylation of maternal pronucleus because of the presence of DPPA3/PGC7 on maternal chromatin that prevents TET3-binding to chromatin. In addition to its role in DNA demethylation, also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT. Binds preferentially to DNA containing cytidine-phosphate-guanosine (CpG) dinucleotides over CpH (H=A, T, and C), hemimethylated-CpG and hemimethylated-hydroxymethyl-CpG.
  • 基因功能參考文獻：
    1. The authors show that Tet3 is selectively up-regulated by activity in hippocampal neurons in vitro, and after formation of fear memory in the hippocampus. This is accompanied by a decrease in miR-29b expression that, through complementary sequences, regulates the level of Tet3 by preferential binding to its 3'UTR. PMID: 29374200
    2. Tet3 displayed differential upregulation after nerve injury, suggesting a potential role in neuropathic pain PMID: 28276837
    3. Tet1/2/3 proteins have a key role in modulating Wnt signaling and establishing the proper balance between neural and mesodermal cell fate determination in mouse embryos and embryonic stem cells PMID: 27930333
    4. results provide novel insight into ATR-mediated DNA damage response, in which TET3-mediated DNA demethylation is crucial for efficient DNA repair and maintenance of genome stability. PMID: 28325772
    5. TET3-5-hydroxymethylcytosine was upregulated in the femoral head tissues of SAON patients and MLO-Y4 cells with dexamethasone (Dex) treatment. PMID: 27627619
    6. TET3 negatively regulates type I IFN production. PMID: 27425624
    7. Tet3, a DNA dioxygenase, can rapidly and efficiently convert fibroblasts directly into functional neurons. PMID: 27880907
    8. Tet enzymes play important roles in telomere maintenance and chromosomal stability of embryonic stem cells by modulating sub-telomeric methylation levels. PMID: 27184841
    9. Here the authors show that concomitant loss of Tet2 and Tet3 in mice at early B cell stage blocked the pro- to pre-B cell transition in the bone marrow, decreased Irf4 expression and impaired the germline transcription and rearrangement of the Igkappa locus. PMID: 27869616
    10. this study shows that simultaneous deletion of Tet2 and Tet3 in mouse CD4+CD8+ double-positive thymocytes results in dysregulated development and proliferation of invariant natural killer T cells PMID: 27869820
    11. The CXXC domain of Tet3 has the capacity to bind to unmethylated and carboxylated cytosines at CpG sequences and a very restricted genomic localization pattern with a preference for transcription start sites. PMID: 26774490
    12. Genetic ablation of TET3 in oocytes had no significant effect on oocyte development. PMID: 26507142
    13. the stability of Foxp3 expression is markedly compromised in T reg cells from Tet2/Tet3 double-deficient mice. PMID: 26903244
    14. The DNA demethylation marks are dynamically regulated in both in vivo and in vitro aging conditions, which are associated with Tet3 over-expression and Tdg repression. PMID: 25982203
    15. Acute elimination of TET function induces the rapid development of an aggressive, fully-penetrant and cell-autonomous myeloid leukaemia in mice, pointing to a causative role for TET loss-of-function in this myeloid malignancy. PMID: 26607761
    16. Knockdown of Tet1 and Tet3 by RNAi in ex vivo cerebellar slice cultures inhibits dendritic arborization of developing cerebellar granule cells, a critical step in circuit formation PMID: 26711116
    17. This paper demonstrates a Tet3-dependent mechanism underlying the dexamethasone-induced epigenetic reprogramming leading to heritable alterations of a fundamental player in cortical development. PMID: 26086966
    18. We propose a mechanism for transcriptional activation in neurons that involves REST-guided targeting of TET3 to the DNA for directed 5hmC generation and NSD3-mediated H3K36 trimethylation PMID: 25843715
    19. results not only reveal a link between miRNAs, TET, and DNA demethylation but also demonstrate critical roles for miR-15b and TET3 in maintaining the NPC pool during early neocortical development. PMID: 25344561
    20. Embryos lacking Tet1 and Tet3 (Tet1/3 DKO) displayed a strong loss of 5-hydroxymethylcytosine (5hmC) and a concurrent increase in 5-methylcytosine (5mC) at the eight-cell stage. PMID: 26199412
    21. Tet3 is important for neural progenitor cell maintenance and terminal differentiation of neurons. PMID: 24838624
    22. Tet3 serves as a synaptic activity sensor to epigenetically regulate fundamental properties and meta-plasticity of neurons via active DNA demethylation PMID: 25915473
    23. TET3 activity protects certain CpG islands against methylation buildup. PMID: 25497087
    24. genome-scale DNA methylation maps for both the paternal and maternal genomes of Tet3-depleted and/or DNA replication-inhibited zygotes, are presented. PMID: 25280220
    25. TET1, TET2 and TET3 mediated DNA demethylation and played role in mouse development. [review] PMID: 25608811
    26. Kidney fibrosis associates with Rasal1 promoter hypermethylation, suppressed Rasal1 expression and suppressed Tet3 expression. PMID: 24480825
    27. Extinction learning-induced Tet3-mediated accumulation of 5-hmC is associated with the establishment of epigenetic states that promote gene expression and rapid behavioral adaptation. PMID: 24757058
    28. TET3 enhanced its localization to chromatin through the stabilization of OGT protein. PMID: 24304661
    29. variable association with CXXC modules may contribute to context specific functions of Tet proteins PMID: 23690950
    30. Data indicate that Tet3 protein level is increased in Tet3-tg expressing olfactory sensory neurons (mOSNs) and colocalizes with increased levels of 5-hydroxymethylcytosine (5hmC). PMID: 23969834
    31. Identifid is a conserved nuclear localization signal (KKRK) embedded between amino acid 1615 and 1618 of mouse TET3. The KKRK sequence facilitates the cytoplasmic protein's translocation into the nucleus. PMID: 23998935
    32. Ascorbate enhances 5-hydroxymethylcytosine generation, most likely by acting as a co-factor for Tet methylcytosine dioxygenases. PMID: 23548903
    33. Aid is a regulator of the subcellular localization of Tet family proteins. PMID: 23028748
    34. Tet3-mediated DNA hydroxylation is involved in epigenetic reprogramming of the zygotic paternal DNA following natural fertilization and may also contribute to somatic cell nuclear reprogramming during animal cloning PMID: 21892189
    35. in addition to 5-hydroxymethylcytosine (5hmC), Tet proteins can generate 5-formylcytosine and 5-carboxylcytosine from 5mC in an enzymatic activity-dependent manner; DNA demethylation may occur through Tet-catalyzed oxidation followed by decarboxylation PMID: 21778364
    36. all three mouse Tet proteins (Tet1, Tet2 and Tet3) can also catalyse conversion of 5-methylcytosine of DNA to 5-hydroxymethylcytosine PMID: 20639862

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  • 亞細胞定位：
    Nucleus. Chromosome. Cytoplasm.
  • 蛋白家族：
    TET family
  • 組織特異性：
    Highly expressed in oocytes and zygotes. Not expressed in embryonic stem cells (ES cells).
  • 數(shù)據(jù)庫鏈接：