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  4. Recombinant Mouse NAD-dependent protein deacetylase sirtuin-3 (Sirt3), partial

Recombinant Mouse NAD-dependent protein deacetylase sirtuin-3 (Sirt3), partial

In Stock Promotion
  • 中文名稱：
    小鼠Sirt3重組蛋白
  • 貨號：
    CSB-EP848095MO
  • 規(guī)格：
    ￥2328
  • 促銷：
    現貨重組蛋白特價促銷
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產品詳情

  • 純度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Partial
  • 來源：
    E.coli
  • 分子量：
    36.3 kDa
  • 表達區(qū)域：
    78-334aa
  • 氨基酸序列
    MVGAGISTPSGIPDFRSPGSGLYSNLQQYDIPYPEAIFELGFFFHNPKPFFMLAKELYPGHYRPNVTHYFLRLLHDKELLLRLYTQNIDGLERASGIPASKLVEAHGTFVTATCTVCRRSFPGEDIWADVMADRVPRCPVCTGVVKPDIVFFGEQLPARFLLHMADFALADLLLILGTSLEVEPFASLSEAVQKSVPRLLINRDLVGPFVLSPRRKDVVQLGDVVHGVERLVDLLGWTQELLDLMQRERGKLDGQDR
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標簽：
    N-terminal 10xHis-tagged and C-terminal Myc-tagged
  • 產品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    3-7 business days
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, PDHA1, SOD2, LCAD, SDHA and the ATP synthase subunit ATP5PO. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels. In response to metabolic stress, deacetylates transcription factor FOXO3 and recruits FOXO3 and mitochondrial RNA polymerase POLRMT to mtDNA to promote mtDNA transcription. Acts as a regulator of ceramide metabolism by mediating deacetylation of ceramide synthases CERS1, CERS2 and CERS6, thereby increasing their activity and promoting mitochondrial ceramide accumulation.
  • 基因功能參考文獻：
    1. genetic manipulation of Sirt3 revealed that amyloid-beta increased levels of total tau acetylated tau through its modulation of Sirt3. PMID: 29574628
    2. data indicate a specific action of the shorter SIRT3 isoform on lipid mitochondrial membrane biosynthesis and functioning. PMID: 29589261
    3. Using the cre-loxP system, we overexpressed the mitochondrial NAD(+) dependent protein deacetylase SIRT3 in enterocytes of mice (iSIRT3 mice). We chronically fed iSIRT3 mice and floxed-SIRT3 control (S3fl) mice a low-fat, control diet (CD) or a high-fat diet (HFD) and then phenotyped the mice. There were no genotype differences in any of the parameters tested when the mice were fed CD. PMID: 29031725
    4. the protective effects of Polydatin are associated with the up-regulation of autophagy and improvement of mitochondrial biogenesis through Sirt3 activity. PMID: 27613967
    5. High-fat-mediated liver damage is associated with Sirt3 downregulation, which is followed by ERK-CREB pathway inactivation and Bnip3-mediated inhibition of mitophagy, causing hepatocytes to undergo mitochondria-dependent cell death. PMID: 30056271
    6. Aerobic interval training (AIT) attenuates high-fat-diet (HFD) -associated cognitive dysfunction through SIRT3 upregulation and improvement of antioxidative MnSOD activity. PMID: 29765980
    7. Low SIRT3 expression is associated with Acute respiratory distress syndrome. PMID: 30171880
    8. SIRT3 inhibited hyperoxia-induced ALI. As a mitochondrial protein, SIRT3 enhanced the expression of MnSOD and reduced the oxidative injury caused by hyperoxic exposure. PMID: 29363727
    9. Activating SIRT3-mediated deacetylation of cyclophilin D attenuated learning and memory dysfunction induced by Sepsis-Associated Encephalopathy. PMID: 28236057
    10. SIRT3 activated mitophagy protecting AFSCs against high glucose-induced apoptosis via preserving mitochondrial function and improved the abnormalities in glucose metabolic parameters, including the levels of glucose, insulin, C-peptide, HbA1c and inflammatory markers. PMID: 29689547
    11. SIRT3 drives Pink1/Parkin activity to increase rates of mitophagy in response to oxidative stress, and thereby limits the production of damaging ROS that would further promote angiogenesis and cardiac remodeling. PMID: 28862956
    12. In this review, the expression of mitochondrial SIRT3 is altered in skeletal muscle of a mouse model of neurodegenerative disease. PMID: 28449871
    13. These results implicate a role of SIRT3 in modulating endothelial function and cardiac function. PMID: 28935506
    14. Results suggest that sirtuin 3 (SIRT3) plays a protective role against mitochondrial damage in the kidney by attenuating reactive oxygen species (ROS) production. PMID: 27620507
    15. SIRT3-deficient macrophages displayed impaired autophagy and accelerated NLRP3 inflammasome activation and endothelial dysfunction. PMID: 29277324
    16. CD38 plays an essential role in cardiac hypertrophy probably via inhibition of SIRT3 expression and activation of Ca(2+) -NFAT signaling pathway. PMID: 28296029
    17. results demonstrate for the first time that melatonin treatment ameliorates myocardial ischemia reperfusion injury by reducing oxidative stress and apoptosis via activating the SIRT3 signaling pathway. PMID: 28500761
    18. The results suggest that melatonin alleviates cardiac remodeling and dysfunction in diabetic cardiomyopathy by upregulating autophagy, limiting apoptosis, and modulating mitochondrial integrity and biogenesis. The mechanisms are associated with Mst1/Sirt3 signaling. PMID: 28480597
    19. EphB2 signaling-mediated Sirt3 expression reduces mesenchymal stem cells senescence by maintaining mitochondrial reactive oxygen species homeostasis. PMID: 28687409
    20. Sirt3 plays a protective role against oxidative stress in oocytes exposed to maternal diabetes through deacetylating SOD2K68. PMID: 28662362
    21. our findings implicate that a novel SIRT3-KLF15 signaling may prevent kidney injury from hypertension and HKL can act as a SIRT3-KLF15 signaling activator to protect against hypertensive nephropathy. PMID: 28465484
    22. Study provides evidence that present that Sirt3 expression protected pancreatic beta cells from lipotoxicity by antagonizing oxidative stress-induced cell damage. PMID: 28375738
    23. The authors provide evidence that inactivation of PIKfyve by the selective inhibitor STA suppresses excessive mitochondrial reactive oxygen species production and apoptosis through a SIRT3-dependent pathway in cardiomyoblasts. PMID: 28396567
    24. Since mitochondrial ATP regeneration is inevitably linked to the maintenance of cardiac pump function, it is not surprising that recent studies revealed a role for mitochondrial sirtuins in the regulation of myocardial energetics and function. In addition, mitochondrial sirtuins modulate the extent of myocardial ischemia reperfusion injury and the development of cardiac hypertrophy and failure. [review] PMID: 27295248
    25. conclude that Sirt3 does not considerably impact NET formation, platelet function, or venous thrombosis in healthy young mice PMID: 29236713
    26. vitamin C and edaravone effectively protected macrophages from stress-induced cytotoxicity, accompanied by downregulated SIRT3 expression and AMPK phosphorylation, and decreased level of autophagy response. Taken together, we conclude that a SIRT3/AMPK/autophagy network orchestrates in the protective effect of resveratrol in macrophages. PMID: 27021965
    27. Sirtuin 3 is required for osteogenic differentiation through maintenance of PGC-1a-SOD2-mediated regulation of mitochondrial function PMID: 28255277
    28. findings reveal an unexpected mechanism for SIRT3 regulation via SIRT1-mediated deacetylation. Improving mitochondrial SIRT3 functions by inhibiting SIRT3 acetylation may offer a new therapeutic approach for obesity- and aging-related diseases associated with mitochondrial dysfunction. PMID: 28808064
    29. Sirt3 expression in bone marrow cells increases during aging, suggesting that Sirt3 promotes age-related adipogenesis and osteoclastogenesis associated with bone loss. These findings identify Sirt3 as an important regulator of adipogenesis and skeletal homeostasis in vivo and identify Sirt3 as a potential target for the treatment of osteoporosis. PMID: 28911171
    30. These data suggest a novel role for SIRT3 deficiency in mediating alveolar epithelial cell mtDNA damage, apoptosis, and lung fibrosis.- PMID: 28258190
    31. Diminished Sirt3 expression and redox inactivation of Sirt3 led to SOD2 inactivation and contributes to hypertension. PMID: 28684630
    32. Results reveal a potential mechanism by which SIRT3 deletion exacerbates post-MI cardiac dysfunction and impairment of cardiac recovery involving microvascular rarefaction and pre-existing coronary microvascular dysfunction. PMID: 27128560
    33. expression and activity of the NADase CD38 increase with aging and that CD38 is required for the age-related NAD decline and mitochondrial dysfunction via a pathway mediated at least in part by regulation of SIRT3 activity. PMID: 27304511
    34. These data demonstrate a critical role of SIRT3 in the control of myofibroblast differentiation and lung fibrosis. PMID: 27815257
    35. NMNAT3 regulates the activity of SIRT3 via synthesis of mitochondria NAD. PMID: 27423420
    36. Data show that peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1alpha) overexpression significantly increased the expression of sirtuin 3 (SIRT3) and sirtuin 5 (SIRT5). PMID: 27052737
    37. These results suggest that the antiinflammatory phenotype of FABP4/aP2 null mice is mediated by increased intracellular monounsaturated fatty acids leading to the increased expression of both uncoupling protein 2 and SirT3. PMID: 26789108
    38. despite pre-existing defects in cardiac function and mitochondrial respiratory capacity in SIRT3(-/-) mice, SIRT3 deficiency does not additionally impair cardiac function following myocardial ischemia-reperfusion injury or following myocardial infarction PMID: 26524632
    39. In the cochlea, the expression of SIRT1, 3, and 5 (both mRNA and protein) was decreased in the old mice PMID: 26472659
    40. SIRT3 plays pivotal roles in adaptive responses of neurons to physiological challenges and resistance to degeneration. PMID: 26698917
    41. Sirt3 activation protects the heart from doxorubicin-induced cardiotoxicity by maintaining OGG1 levels and protecting mitochondria from DNA damage. PMID: 26873966
    42. CerS1, -2, and -6 are hyperacetylated in the mitochondria of SIRT3-null mice. PMID: 26620563
    43. as compared to Sirt3 overexpressed and normal groups, the acetylation of IDH2 and accumulation of ox-LDL were significantly higher in the Sirt3 inhibited group PMID: 26464666
    44. Sirt3-dependent deacetylation of SOD2 plays a protective role against oxidative stress and meiotic defects in oocytes under maternal obese conditions. PMID: 25790176
    45. A mouse model of developmentally primed nonalcoholic steatohepatitis is c characterized by an altered cellular redox status, sirtuin abundance and clock gene expression. PMID: 27040510
    46. Sir3 dysfunction in Cmah-null mice altered various signaling pathways, which are responsible for age-related hearing loss. PMID: 26319214
    47. Ischemic postconditioning might prevent lethal reperfusion injury through an increased SIRT3 activity and subsequent attenuation of CyPD acetylation at reperfusion. PMID: 25871830
    48. SIRT3 and SIRT5 regulate the enzyme activity and cardiolipin binding of very long-chain acyl-CoA dehydrogenase PMID: 25811481
    49. This study demonstrates the importance of SIRT3 in preserving heart function and capillary density in the setting of obesity. PMID: 25782072
    50. SIRT3 functions as a protein repair factor that removes acetylation lesions from lysine residues. PMID: 26358839

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  • 亞細胞定位：
    [Isoform L]: Mitochondrion matrix.; [Isoform S]: Cytoplasm.
  • 蛋白家族：
    Sirtuin family, Class I subfamily
  • 組織特異性：
    Strongly expressed in liver and kidney. Expressed in skeletal muscles (at protein level). Weakly expressed in lung.
  • 數據庫鏈接：