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  4. Recombinant Mouse Negative elongation factor B (Cobra1)

Recombinant Mouse Negative elongation factor B (Cobra1)

  • 中文名稱：
    小鼠Nelfb重組蛋白
  • 貨號(hào)：
    CSB-YP804914MO
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Nelfb重組蛋白
  • 貨號(hào)：
    CSB-EP804914MO
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Nelfb重組蛋白
  • 貨號(hào)：
    CSB-EP804914MO-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Nelfb重組蛋白
  • 貨號(hào)：
    CSB-BP804914MO
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Nelfb重組蛋白
  • 貨號(hào)：
    CSB-MP804914MO
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Nelfb
  • Uniprot No.：
  • 別名：
    Nelfb; Cobra1; MNCb-5210Negative elongation factor B; NELF-B; Cofactor of BRCA1
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長(zhǎng)度：
    full length protein
  • 表達(dá)區(qū)域：
    1-580
  • 氨基酸序列
    MFAGLQDLGV ANGEDLKETL TNCTEPLKAI EQFQTENGVL LPSLQSALPF LDLHGTPRLE FHQSVFDELR DKLLERVSAI ASEGKAEERY KKLEDLLEKS FSLVKMPSLQ PVVMCVMKHL PKVPEKKLKL VMADKELYRA CAVEVKRQIW QDNQALFGDE VSPLLKQYIL EKESALFSTE LSVLHNFFSP SPKTRRQGEV VQKLTQMVGK NVKLYDMVLQ FLRTLFLRTR NVHYCTLRAE LLMSLHDLDV SDICTVDPCH KFTWCLDACI RERFVDSKRA RELQGFLDGV KKGQEQVLGD LSMILCDPFA INTLSLSTIR HLQELVSQET LPRDSPDLLL LLRLLALGQG AWDLIDSQVF KEPKMEAELI TKFLPMLMSL VVDDFTFNVD QKLPAEEKAS VTYPNTLPES FTKFLQEQRM ACEVGLYYVL HITKQRNKNA LLRLLPGLVE TFGDLAFSDI FLHLLTGSLV LLADEFALED FCSSLFDGFF LTASPRKENV HRHVLRLLLH LHARVAPSKL EALQKALEPT GQSGEAVKEL YSQLGEKLEQ LDHRKPSPTQ AAETPALDLP LPSVPAPATL
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II (Pol II). The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. May be able to induce chromatin unfolding. Essential for early embryogenesis; plays an important role in maintaining the undifferentiated state of embryonic stem cells (ESCs) by preventing unscheduled expression of developmental genes. Plays a key role in establishing the responsiveness of stem cells to developmental cues; facilitates plasticity and cell fate commitment in ESCs by establishing the appropriate expression level of signaling molecules. Supports the transcription of genes involved in energy metabolism in cardiomyocytes; facilitates the association of transcription initiation factors with the promoters of the metabolism-related genes.
  • 基因功能參考文獻(xiàn)：
    1. BRCA1 Interacting Protein COBRA1 Facilitates Adaptation to Castrate-Resistant Growth Conditions. PMID: 30036938
    2. This study uncovers a previously unappreciated, DNA repair-independent function of BRCA1 in antagonizing COBRA1-dependent transcription program during mammary gland development. PMID: 26941120
    3. both human and mouse NELF-B proteins are translated from a non-AUG codon upstream of the annotated AUG. PMID: 26010750
    4. Human and mouse Nelfb encode proteins translated from a non-AUG (CUG) start codon. The majority of mouse tissues examined express the isoform translated from the CUG start codon, but a small number of tissues also express a shorter isoform of the same apparent size as the AUG-initiated isoform. PMID: 26010750
    5. Cobra1 Is Required for TrkC-Induced Apoptosis. PMID: 24034695
    6. genetic and genomic studies provide new physiological and molecular insight into Nelf-mediated pol II pausing. PMID: 21865163
    7. findings also indicate that Cobra1 helps maintain the undifferentiated state of mESCs by preventing unscheduled expression of developmental genes PMID: 19340312

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  • 亞細(xì)胞定位：
    Nucleus.
  • 蛋白家族：
    NELF-B family
  • 組織特異性：
    Isoform 1 is expressed in the kidney, liver, adipose and lung. Isoform 2 is widely expressed.
  • 數(shù)據(jù)庫(kù)鏈接：