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  4. Recombinant Mouse Period circadian protein homolog 2 (Per2), partial

Recombinant Mouse Period circadian protein homolog 2 (Per2), partial

  • 中文名稱：
    小鼠Per2重組蛋白
  • 貨號(hào)：
    CSB-YP017787MO
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Per2重組蛋白
  • 貨號(hào)：
    CSB-EP017787MO
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Per2重組蛋白
  • 貨號(hào)：
    CSB-EP017787MO-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Per2重組蛋白
  • 貨號(hào)：
    CSB-BP017787MO
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Per2重組蛋白
  • 貨號(hào)：
    CSB-MP017787MO
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Per2
  • Uniprot No.：
  • 別名：
    Per2; Period circadian protein homolog 2; mPER2; Circadian clock protein PERIOD 2
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長(zhǎng)度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Transcriptional repressor which forms a core component of the circadian clock. The circadian clock, an internal time-keeping system, regulates various physiological processes through the generation of approximately 24 hour circadian rhythms in gene expression, which are translated into rhythms in metabolism and behavior. It is derived from the Latin roots 'circa' (about) and 'diem' (day) and acts as an important regulator of a wide array of physiological functions including metabolism, sleep, body temperature, blood pressure, endocrine, immune, cardiovascular, and renal function. Consists of two major components: the central clock, residing in the suprachiasmatic nucleus (SCN) of the brain, and the peripheral clocks that are present in nearly every tissue and organ system. Both the central and peripheral clocks can be reset by environmental cues, also known as Zeitgebers (German for 'timegivers'). The predominant Zeitgeber for the central clock is light, which is sensed by retina and signals directly to the SCN. The central clock entrains the peripheral clocks through neuronal and hormonal signals, body temperature and feeding-related cues, aligning all clocks with the external light/dark cycle. Circadian rhythms allow an organism to achieve temporal homeostasis with its environment at the molecular level by regulating gene expression to create a peak of protein expression once every 24 hours to control when a particular physiological process is most active with respect to the solar day. Transcription and translation of core clock components (CLOCK, NPAS2, ARNTL/BMAL1, ARNTL2/BMAL2, PER1, PER2, PER3, CRY1 and CRY2) plays a critical role in rhythm generation, whereas delays imposed by post-translational modifications (PTMs) are important for determining the period (tau) of the rhythms (tau refers to the period of a rhythm and is the length, in time, of one complete cycle). A diurnal rhythm is synchronized with the day/night cycle, while the ultradian and infradian rhythms have a period shorter and longer than 24 hours, respectively. Disruptions in the circadian rhythms contribute to the pathology of cardiovascular diseases, cancer, metabolic syndrome and aging. A transcription/translation feedback loop (TTFL) forms the core of the molecular circadian clock mechanism. Transcription factors, CLOCK or NPAS2 and ARNTL/BMAL1 or ARNTL2/BMAL2, form the positive limb of the feedback loop, act in the form of a heterodimer and activate the transcription of core clock genes and clock-controlled genes (involved in key metabolic processes), harboring E-box elements (5'-CACGTG-3') within their promoters. The core clock genes: PER1/2/3 and CRY1/2 which are transcriptional repressors form the negative limb of the feedback loop and interact with the CLOCK|NPAS2-ARNTL/BMAL1|ARNTL2/BMAL2 heterodimer inhibiting its activity and thereby negatively regulating their own expression. This heterodimer also activates nuclear receptors NR1D1/2 and RORA/B/G, which form a second feedback loop and which activate and repress ARNTL/BMAL1 transcription, respectively. PER1 and PER2 proteins transport CRY1 and CRY2 into the nucleus with appropriate circadian timing, but also contribute directly to repression of clock-controlled target genes through interaction with several classes of RNA-binding proteins, helicases and others transcriptional repressors. PER appears to regulate circadian control of transcription by at least three different modes. First, interacts directly with the CLOCK-ARTNL/BMAL1 at the tail end of the nascent transcript peak to recruit complexes containing the SIN3-HDAC that remodel chromatin to repress transcription. Second, brings H3K9 methyltransferases such as SUV39H1 and SUV39H2 to the E-box elements of the circadian target genes, like PER2 itself or PER1. The recruitment of each repressive modifier to the DNA seems to be very precisely temporally orchestrated by the large PER complex, the deacetylases acting before than the methyltransferases. Additionally, large PER complexes are also recruited to the target genes 3' termination site through interactions with RNA-binding proteins and helicases that may play a role in transcription termination to regulate transcription independently of CLOCK-ARTNL/BMAL1 interactions. Recruitment of large PER complexes to the elongating polymerase at PER and CRY termination sites inhibited SETX action, impeding RNA polymerase II release and thereby repressing transcriptional reinitiation. May propagate clock information to metabolic pathways via the interaction with nuclear receptors. Coactivator of PPARA and corepressor of NR1D1, binds rhythmically at the promoter of nuclear receptors target genes like ARNTL or G6PC1. Directly and specifically represses PPARG proadipogenic activity by blocking PPARG recruitment to target promoters and thereby transcriptional activation. Required for fatty acid and lipid metabolism, is involved as well in the regulation of circulating insulin levels. Plays an important role in the maintenance of cardiovascular functions through the regulation of NO and vasodilatatory prostaglandins production in aortas. Controls circadian glutamate uptake in synaptic vesicles through the regulation of VGLUT1 expression. May also be involved in the regulation of inflammatory processes. Represses the CLOCK-ARNTL/BMAL1 induced transcription of BHLHE40/DEC1 and ATF4. Negatively regulates the formation of the TIMELESS-CRY1 complex by competing with TIMELESS for binding to CRY1.
  • 基因功能參考文獻(xiàn)：
    1. Studied roles of CK1delta and CK1e in regulation of PER2 in circadian rhythm. PMID: 29784789
    2. Collectively, SIK3 plays key roles in circadian rhythms by facilitating phosphorylation-dependent PER2 destabilization, either directly or indirectly. PMID: 29227248
    3. The regulatory role of Per2 3'- UTR in circadian rhythm and PER2 protein accumulation. PMID: 28973913
    4. proposal of models to determine the regulatory role Per2AS plays in the mammalian circadian clock and potential effects of interactions between Per2 and Per2AS RNAs on circadian rhythms PMID: 29447160
    5. PML mediates the binding of PER2 to BMAL1 in the BMAL1/CLOCK heterodimer and is an important component in the organization of a functional clock complex in the nucleus. PMID: 27383066
    6. PER2 is required for proper mouse mammary gland development and thus has a crucial role outside of its circadian function. PMID: 29490985
    7. Data suggest that, in the SCN (suprachiasmatic nucleus), the day-night difference in PER2 expression is similar between chow-fed and fcHFHS-fed mice; in the LHb (epithalamic lateral habenula), this day-night difference is altered in fcHFHS-fed mice compared to chow-fed mice. (fcHFHS = free choice high-fat high-sugar diet) PMID: 28687372
    8. The interplay between Sirt1 and Per2 modulates aging gene expression and circadian-clock maintenance. PMID: 27346580
    9. bioluminescence rhythm of peripheral clocks in PER2::LUC mice was strongly entrained by forced treadmill and forced wheel-running exercise rather than by voluntary wheel-running exercise at middle time during the inactivity period. Exercise-induced entrainment was accompanied by increased levels of serum corticosterone and norepinephrine in peripheral tissues, similar to the physical stress-induced response PMID: 27271267
    10. Expression of Per2 and Bmal1 in the ovary did not display clear diurnal oscillation. PMID: 28213822
    11. Per2 acted here as a liver tumor suppressor from initiation to progression. PMID: 27494874
    12. A knockin mouse expressing a fluorescent fusion of native PER2 protein (PER2::VENUS) for live imaging showed how the mobility and nuclear translocation of PER2 are regulated by casein kinase. PMID: 27374340
    13. Data indicate that following removal of tetrodotoxin (TTX), the dorsal region attained a stable period after the ventral region and an earlier time of peak PERIOD2 (PER2) expression. PMID: 28326909
    14. Data show that extracellular luciferin concentration significantly affects the apparent circadian amplitude and phase of PER2::LUC reporter in cultured fibroblasts, but not in suprachiasmatic nucleus (SCN). PMID: 28112045
    15. The Per2 function in the bone marrow is required for the regulation of life span in circulating erythrocytes. PMID: 28607147
    16. miR-21 as cardioprotective downstream target of Per2 PMID: 28448534
    17. these results identify Per2 as a negative regulator of Ly-biased haematopoietic stem cells and immune functions in response to DNA damage and ageing. PMID: 27088856
    18. Circadian PER2 rhythm in the olfactory bulb of freely moving mice is organized by the nucleus circadian pacemaker, and is independent of the circadian behavioral rhythm PMID: 26489367
    19. polyamines control the circadian period in cultured cells and animals by regulating the interaction between the core clock repressors PER2 and CRY1 PMID: 26456331
    20. Decreased Bone Volume and Bone Mineral Density in the Tibial Trabecular Bone Is Associated with Per2 Gene by 405 nm Laser Stimulation PMID: 26580614
    21. Data show that early doors period 2 (PER2) I324N mutation (Per2 Edo) reveals a direct causal link between the molecular structure of the PER2 PAS domain and the pace of suprachiasmatic nucleus (SCN) circadian timekeeping. PMID: 26903623
    22. PER2 ablation in the liver abolishes food anticipation via decreased beta-hydroxybutyrate production. PMID: 26838474
    23. hnRNP C and hnRNP H bind to the 3'-UTR of the circadian clock gene Period 2 (mPer2). PMID: 25846207
    24. clock genes (such as Per2) constitute a common pathway mediating the shared and well-known adverse effects of both chronic sleep loss and disrupted circadian rhythmicity on metabolic health PMID: 25581923
    25. Results demonstrate that the two-site phosphoswitch of PER2 regulates circadian timekeeping by controlling PER2 stability. PMID: 26431025
    26. Abeta-induced degradation of BMAL1 and CBP correlated with the reduced binding of transcription factors to the Per2 promoter PMID: 25888034
    27. each layer of the retina in the mPer2(Luc) mouse harbors a self-sustained oscillator whose period is significantly longer ( approximately 26 hours) than in whole-retina explants ( approximately 22.9 hours), indicating that the period is correlated with the degree of coupling. PMID: 25573753
    28. Data show that the intermolecular zinc finger is important for period circadian protein (PER2)-cryptochrome 2 (CRY2) complex formation. PMID: 25127877
    29. Per2 modulates EPC mobilization and function after myocardial infarction, which is important to recovery after myocardial infarction in mice. PMID: 25268972
    30. Circadian oscillations of PER2::LUC bioluminescence aligned with Per2 mRNA expression upon analysis using quantitative PCR PMID: 24997189
    31. Per2 may be a key factor in maintaining endothelial progenitor cell function in vitro and in therapeutic angiogenesis in vivo. PMID: 24621388
    32. Daily activity of Per2 mutant mice appears not to respond to the delaying action of light in the late subjective day. PMID: 24527953
    33. Rhythmic PER2 activities occurred in the bladder wall with a cycle of approximately 24 h and peak at approximately 12 h PMID: 25145629
    34. Data show that Ser557 phosphorylation of CRY2 promotes CRY2 degradation and inhibits the overaccumulation of the CRY2-PER2 complex in the nucleus. PMID: 25288642
    35. Data indicate that the medium changes occurring 1 to 12 h after the peak of Period2 protein (PER2)::LUC expression advanced the phase of the rhythms, whereas medium changes at other times delayed the rhythm. PMID: 24498336
    36. Data indicate that sevoflurane suppresses the expression of Period2 (Per2) mRNA in the suprachiasmatic nucleus (SCN). PMID: 24498074
    37. a stress-induced phase shift in a peripheral clock gene Per2 rhythm PMID: 24829500
    38. The absence of PERIOD2 abolishes the gating of cell-cycle entrance of quiescent neural progenitor cells. PMID: 24268780
    39. Per2-deficient mice are reminiscent more of accelerated aging rather than tumor-prone phenotype. PMID: 24091726
    40. Study determined the crystal structure of a complex comprising the photolyase homology region of mouse CRY1 (mCRY1) and a C-terminal mouse PER2 (mPER2) fragment and provides evidence that mCRY1/mPER2 complex formation is modulated by an interplay of zinc binding and mCRY1 disulfide bond formation, which may be influenced by the redox state of the cell. PMID: 24855952
    41. p53 regulates Per2 expression and the circadian clock. PMID: 24051492
    42. These studies reveal an important role of cardiac Per2 for fatty acid metabolism and inflammation during myocardial ischemia and reperfusion, respectively. PMID: 23977055
    43. Folate deficiency leads to dampened PER2 and vasopressin oscillations in the master clock. PMID: 23273571
    44. data suggest that early-life exposure to alcohol alters metabolic sensing to the hypothalamus possibly via regulating Per2 gene. PMID: 22823489
    45. mPer2 deletion preserves mitochondrial membrane structure and functional integrity in heart following ischemia-reperfusion injury. PMID: 23771689
    46. We found greatly reduced PER2 rhythmicity in low-density cultures, which could result from lack of either constitutive or rhythmic paracrine signals from neighboring fibroblasts PMID: 23735497
    47. Our observations indicated that clock gene Per2 plays a protective role in mediating liver injury and fibrosis during cholestasis. PMID: 22261359
    48. These results suggested that the Per2 clock gene is not necessary for the photoperiodic response in mice. PMID: 23505514
    49. Per2 plays a well-established role in circadian rhythms. PMID: 23450268
    50. The Per2 mutant mice have a cancer prone phenotype and an altered DNA damage response PMID: 22905719

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  • 亞細(xì)胞定位：
    Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Note=Nucleocytoplasmic shuttling is effected by interaction with other circadian core oscillator proteins and/or by phosphorylation. Translocate to the nucleus after phosphorylation by CSNK1D or CSNK1E. Also translocated to the nucleus by CRY1 or CRY2. PML regulates its nuclear localization.
  • 組織特異性：
    In the brain, high expression in SCN during the subjective day. Constitutive expression in the cornu ammonis and in the dentate gyrus of the hyppocampus. Also expressed in the piriform cortex and the glomeruli of the olfactory bulb, and at a lower extent
  • 數(shù)據(jù)庫(kù)鏈接：