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Recombinant Mouse Phospholipid transfer protein (Pltp)

  • 中文名稱：
    小鼠Pltp重組蛋白
  • 貨號：
    CSB-EP018212MO
  • 規(guī)格：
    ￥1536
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Full Length of Mature Protein
  • 來源：
    E.coli
  • 分子量：
    56.7 kDa
  • 表達(dá)區(qū)域：
    18-493aa
  • 氨基酸序列
    ELPGCKIRVTSAALDLVKQEGLRFLEQELETITIPDVYGAKGHFYYNISDVRVTQLHLISSELHFQPDQDLLLNISNASLGLHFRRQLLYWFLYDGGYINASAEGVSIRTGLQLSQDSSGRIKVSNVSCEASVSKMNMAFGGTFRRMYNFFSTFITSGMRFLLNQQICPVLYHAGTVLLNSLLDTVPVRSSVDDLVGIDYSLLKDPVVSNGNLDMEFRGAFFPLKEDNWSLPNRAVEPQLEDDERMVYVAFSEFFFDSAMESYFQAGALQLTLVGDKVPSDLDMLLRATYFGSIVLLSPTVINSPLKLKLEATSPPRCTIKPSGTTISITASVTITLAPPMLPEVELSKMIMEGRLSAKLTLRGKALRVKLDLRRFQIYSNQSALESLALIPLQAPLKTLLQIGVMPLLNERTWRGVQIPLPEGINFVREVVTNHAGFVTVGADLHFAKGLREVIDKNRPADVAASHVPPPSAAAA
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標(biāo)簽：
    N-terminal 6xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Mediates the transfer of phospholipids and free cholesterol from triglyceride-rich lipoproteins (low density lipoproteins or LDL and very low density lipoproteins or VLDL) into high-density lipoproteins (HDL) as well as the exchange of phospholipids between triglyceride-rich lipoproteins themselves. Facilitates the transfer of a spectrum of different lipid molecules, including sphingomyelin, phosphatidylcholine, phosphatidylinositol, phosphatidylglycerol, and phosphatidyl ethanolamine. Plays an important role in HDL remodeling which involves modulating the size and composition of HDL. Also plays a key role in the uptake of cholesterol from peripheral cells and tissues that is subsequently transported to the liver for degradation and excretion. Two distinct forms of PLTP exist in plasma: an active form that can transfer phosphatidylcholine from phospholipid vesicles to HDL, and an inactive form that lacks this capability.
  • 基因功能參考文獻(xiàn)：
    1. this work reports a physiological role for PLTP in the polarization of CD4(+) T cells toward the pro-inflammatory Th1 phenotype. PMID: 26320740
    2. these findings elucidated that PLTP repressed LPS induced inflammation due to extracellular LPS binding capability, and the protective effects were not related to HDL pool size in mice. PMID: 26857615
    3. Data presented a novel model to link phospholipid metabolism to APP processing and also suggested that PLTP played an important role in Abeta metabolism and would be useful to further elucidate functions of PLTP in Alzheimer's disease susceptibility. PMID: 26160914
    4. In hepatocytes, initial recruitment of phospholipid (PL) by apoB:1000 leading to the formation of the PL-rich apoB-containing initiation complex is mediated to a large extent by PLTP. PMID: 25638820
    5. PLTP plays an important role in modulating the stability of atherosclerotic plaques. The receptor-interacting protein 3- reactive oxygen species signal pathway could be involved in this PLTP-mediated process. PMID: 25324570
    6. Adipocyte PLTP plays a small but significant role in plasma PLTP activity and promotes cholesterol efflux from adipose tissues. PMID: 25477345
    7. PLTP exerts significant effects on apoA-I lipidation and nascent HDL biogenesis in hepatocytes by promoting ATP-binding cassette transporter A1-mediated lipid efflux and the remodeling of nascent HDL particles. PMID: 25060793
    8. Plasma Sphingosine-1-phosphate contents were decreased by 60.1 % in PLTP knockout mice (PLTP-/-, N = 5) compared with their wild type littermates (WT, N = 5) (151.70 +/- 38.59 vs. 379.32 +/- 59.90 nmol/l, P<0.01). PMID: 24158769
    9. Therefore we concluded that PLTP deficiency impaired cognition and aggravated AD by enhancing the generation of Abeta in the cortex of old mice. PMID: 24121956
    10. Cerebrovascular oxidative stress increased in PLTP deficient mice, including increased levels of reactive oxygen species (ROS) and lipid peroxidation marker 4-hydroxy-2-nonenal (HNE) and reduced superoxide dismutase (SOD) activity. PMID: 24513285
    11. PLTP is actively involved in lipid transfer, cholesterol efflux, HDL genesis, and remodeling at the blood-brain barrier. PMID: 24369175
    12. PLTP can play a significant role in the pathophysiology of abdominal aortic aneurysm. PMID: 23830874
    13. Liver-specific PLTP deficiency significantly reduces plasma HDL and apoB-containing lipoprotein levels. PMID: 23846500
    14. The results of this study suggested that PLTP, through its ability to deliver vitamin E to the brain, constitutes an endogenous neuroprotective agent. Increasing PLTP activity may offer a new way to develop neuroprotective therapies. PMID: 23303044
    15. Our results suggest, for the first time, that the major function of liver PLTP is to drive VLDL production and makes a small contribution to plasma PLTP activity. PMID: 22367708
    16. presence of PLTP in tear fluid appears to be essential for maintaining a healthy and functional ocular surface PMID: 21514421
    17. the SR-BI pathway contributes in unique ways to cholesterol metabolism and atherosclerosis susceptibility even in the presence of CETP PMID: 21454568
    18. Diet-induced lipid accumulation in phospholipid transfer protein-deficient mice: its atherogenicity and potential mechanism PMID: 20543142
    19. PLTP increases blood coagulation and worsens the extent of ischemic lesions in response to acute oxidative stress. PMID: 20864671
    20. It is concluded that PLTP is essential in mediating the association of triacyl lipid A with lipoproteins, leading to extension of its residence time and to magnification of its proinflammatory and anticancer properties. PMID: 20418497
    21. increased PLTP permits triglyceride incorporation into abnormally large VLDL, which are removed from plasma by LDL receptors PMID: 20037162
    22. data show that PLTP regulates the bioavailability of vitamin E in atherogenic lipoproteins and suggest a novel strategy for achieving more effective concentrations of anti-oxidants in lipoproteins, independent of dietary supplementation PMID: 12105225
    23. pltp is regulated by liver X receptors in mice PMID: 12177004
    24. elevated PLTP activity in human cholesteryl ester transfer protein (huCEPT) transgenic mice results in an increase in VLDL secretion PMID: 12401886
    25. The phospholipid transfer protein gene is a liver X receptor target expressed by macrophages in atherosclerotic lesions. PMID: 12612088
    26. Two-fold increased plasma PLTP activity in apoE knockout mice results in a decrease in HDL cholesterol, HDL phospholipid, and apoAI levels and indicates that elevated plasma PLTP levels constitute a novel, long-term risk factor for atherosclerosis. PMID: 12855484
    27. PLTP has a role in transporting cholesterol and phospholipids from cells to lipoprotein particles by a process involving PLTP interactions with cellular ATP-binding cassette transporter A1 PMID: 14559902
    28. Five significant loci were identified as being involved in PLTP activity in the mouse and supporting evidence was provided for the candidacy of Nr1h4 and Apof as the genes underlying Pltpq2. PMID: 14592843
    29. LDL from PLTP0 mice was markedly resistant to oxidation and induced significantly less monocyte chemotactic activity compared with that in controls. In vitro, PLTP0 HDL removed significantly more oxidized phospholipids from LDL than did control HDL PMID: 15258196
    30. PLTP deficiency decreases liver vitamin E content, increases hepatic oxidant tone, and substantially enhances reactive oxygen species-dependent destruction of newly synthesized apolipoprotein B via a post-endoplasmic reticulum process. PMID: 15734742
    31. data suggested that PLTP may play important role(s) during in vivo preimplantation embryo development PMID: 15832314
    32. The effect of PLTP gene deletion on the fertility of male and female mice is reported. PMID: 16467369
    33. complex regulatory role of PLTP in serum and liver lipid homeostasis, as well as in the formation of nascent apoA-I-PL complexes from the liver PMID: 16554055
    34. Absence of endogenous phospholipid transfer protein impairs ABCA1-dependent efflux of cholesterol from macrophage foam cells. PMID: 16687660
    35. Macrophage PLTP deficiency causes a significant reduction of apoE secretion from the cells, and this in turn promotes the accumulation of cholesterol in the circulation and accelerates the development of atherosclerosis. PMID: 17038631
    36. PLTP deficiency in knockout mice results in reduced cholesterol uptake as well as secretion by the intestine. PMID: 17641249
    37. antiatherogenic properties of macrophage-derived PLTP are related at least in part to its ability to reduce cholesterol accumulation in macrophages through changes in the alpha-tocopherol content and oxidative status of the cells PMID: 17717294
    38. unlike total systemic PLTP, locally produced macrophage-derived PLTP beneficially alters lipoprotein metabolism and reduces lesion progression in hyperlipidemic mice PMID: 17928634
    39. PLTP appears to play a proatherogenic role in diet-induced hyperlipidemic mice PMID: 18198166
    40. PLTP expression by bone marrow derived cells results in atherogenic effects on plasma lipids, increased PLTP activity, high local PLTP protein levels in the atherosclerotic lesions and increased atherosclerotic lesion size PMID: 18509527
    41. PLTP transfer activity is essential for the development of atherosclerosis in PLTP transgenic mice. PMID: 18711210
    42. These findings indicate that plasma HDL level is an important regulator of plasma PLTP and might play a role in the stabilization of PLTP in plasma. PMID: 19422933
    43. Elevated PLTP activity lowers plasma HDL-cholesterol by stimulating the plasma turnover and hepatic uptake of HDL cholesteryl esters. PMID: 19524061

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  • 亞細(xì)胞定位：
    Secreted. Nucleus.
  • 蛋白家族：
    BPI/LBP/Plunc superfamily, BPI/LBP family
  • 組織特異性：
    Highest level expression in the lung, brain and heart with relatively low levels in the liver, skeletal muscle and testis and very low levels found in the spleen and kidney.
  • 數(shù)據(jù)庫鏈接：