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  4. Recombinant Mouse Poly [ADP-ribose] polymerase 9 (Parp9), partial

Recombinant Mouse Poly [ADP-ribose] polymerase 9 (Parp9), partial

  • 中文名稱：
    小鼠Parp9重組蛋白
  • 貨號：
    CSB-YP807480MO
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Parp9重組蛋白
  • 貨號：
    CSB-EP807480MO
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Parp9重組蛋白
  • 貨號：
    CSB-EP807480MO-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Parp9重組蛋白
  • 貨號：
    CSB-BP807480MO
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Parp9重組蛋白
  • 貨號：
    CSB-MP807480MO
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Parp9
  • Uniprot No.：
  • 別名：
    Parp9; Bal; Protein mono-ADP-ribosyltransferase PARP9; EC 2.4.2.-; ADP-ribosyltransferase diphtheria toxin-like 9; ARTD9; B aggressive lymphoma protein homolog; Poly [ADP-ribose] polymerase 9; PARP-9
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    ADP-ribosyltransferase which, in association with E3 ligase DTX3L, plays a role in DNA damage repair and in immune responses including interferon-mediated antiviral defenses. Within the complex, enhances DTX3L E3 ligase activity which is further enhanced by PARP9 binding to poly(ADP-ribose). In addition, positively regulates DTXL3 protein levels. In association with DTX3L and in presence of E1 and E2 enzymes, mediates NAD(+)-dependent mono-ADP-ribosylation of ubiquitin which prevents ubiquitin conjugation to substrates such as histones. During DNA repair, PARP1 recruits PARP9/BAL1-DTX3L complex to DNA damage sites via PARP9 binding to ribosylated PARP1. Subsequent PARP1-dependent PARP9/BAL1-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites. In response to DNA damage, PARP9-DTX3L complex is required for efficient non-homologous end joining (NHEJ) but the complex function is restrained by PARP9 activity. Dispensable for B-cell receptor (BCR) assembly through V(D)J recombination and class switch recombination (CSR). In macrophages, positively regulates pro-inflammatory cytokines production in response to IFNG stimulation by suppressing PARP14-mediated STAT1 ADP-ribosylation and thus promoting STAT1 phosphorylation. Also suppresses PARP14-mediated STAT6 ADP-ribosylation.
  • 基因功能參考文獻(xiàn)：
    1. PARP9 and PARP14 regulate macrophage activation in macrophage cell lines treated with either IFNgamma or IL-4; PARP14 silencing induces pro-inflammatory genes and STAT1 phosphorylation in M(IFNgamma) cells, whereas it suppresses anti-inflammatory gene expression and STAT6 phosphorylation in M(IL-4) cells PMID: 27796300
    2. this study shows that Parp9-deficient mice are viable, fertile, and do not show any overt phenotype; Parp9 is dispensable for B-cell development, and that Parp9 is not essential for double-stranded DNA break repair, or that strong compensatory mechanisms exist PMID: 28105679
    3. Data indicate that PARP polymerase PARP9-E3 ligase DTX3L interacts with STAT1 transcription factor and enhances translocation of STAT1 to the nucleus. PMID: 26479788
    4. Data show that Parp-9 is developmentally regulated, and prominently expressed in the thymus and specific regions of the brain and gut. PMID: 18069692
  • 亞細(xì)胞定位：
    Cytoplasm, cytosol. Nucleus.
  • 組織特異性：
    Highly expressed in the thymus and intestine. Expressed in macrophages.
  • 數(shù)據(jù)庫鏈接：