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  4. Recombinant Mouse Serine/threonine-protein kinase B-raf (Braf), partial

Recombinant Mouse Serine/threonine-protein kinase B-raf (Braf), partial

  • 中文名稱：
    Recombinant Mouse Serine/threonine-protein kinase B-raf(Braf) ,partial,Yeast
  • 貨號：
    CSB-YP002791MO
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Mouse Serine/threonine-protein kinase B-raf(Braf) ,partial,Yeast
  • 貨號：
    CSB-EP002791MO
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Mouse Serine/threonine-protein kinase B-raf(Braf) ,partial,Yeast
  • 貨號：
    CSB-EP002791MO-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Mouse Serine/threonine-protein kinase B-raf(Braf) ,partial,Yeast
  • 貨號：
    CSB-BP002791MO
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Mouse Serine/threonine-protein kinase B-raf(Braf) ,partial,Yeast
  • 貨號：
    CSB-MP002791MO
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Braf
  • Uniprot No.：
  • 別名：
    Braf; B-raf; Serine/threonine-protein kinase B-raf; EC 2.7.11.1; Proto-oncogene B-Raf
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway. May play a role in the postsynaptic responses of hippocampal neurons.
  • 基因功能參考文獻：
    1. Persistent oncogenic Braf signaling is sufficient to induce widespread DNA methylation and colorectal neoplasia. PMID: 29235923
    2. Results indicate specific and compensatory functions for proto-oncogene B-Raf (BRAF) and proto-oncogene c-RAF (CRAF) and highlight an addiction to RAF signalling in NRAS-driven melanoma. PMID: 28497782
    3. Authors report that BrafQ241R/+ mice have neonatal feeding difficulties and esophageal dilation. The esophagus tissues from BrafQ241R/+ mice displayed incomplete replacement of smooth muscle with skeletal muscle and decreased contraction. PMID: 28973166
    4. mosaic expression of BRAF(V600E) in mouse erythro-myeloid progenitors results in clonal expansion of tissue-resident macrophages and a severe late-onset neurodegenerative disorder PMID: 28854169
    5. CDX2(Null)/BRAF(V600E) expression in adult mouse intestinal epithelium led to serrated morphology tumors (including carcinomas) and BRAF(V600E) potently interacted with CDX2 silencing to alter gene expression. Like human serrated lesions, CDX2(Null)/BRAF(V600E)-mutant epithelium expressed gastric markers. PMID: 28072391
    6. expression of an endogenous Braf(D631A) kinase-inactive isoform in mice (corresponding to the human BRAF(D594A) mutation) triggers lung adenocarcinoma in vivo, indicating that BRAF-inactivating mutations are initiating events in lung oncogenesis PMID: 28783725
    7. TTM reduces copper levels and MAPK signaling, thereby inhibiting BRAF(V600E)-driven melanoma tumor growth. PMID: 28986383
    8. BRAF and ROKalpha form independent RAF1 complexes in embryonic fibroblasts (MEFs) treated with epidermal growth factor (EGF). PMID: 28270557
    9. Braf(V600E) expression, coupled with simultaneous p53 ablation, permits bypass of senescence and progression to lung adenocarcinoma. PMID: 28745322
    10. These results provide support for the role of BRAF(V600E) in metastasis. PMID: 27210749
    11. Mechanistically, BRAF and RAF1 operate independently to balance MAPK signaling: BRAF promotes ERK activation, while RAF1 dims stress kinase activation. PMID: 27431613
    12. Mass spectrometry based screening for potential interaction partners revealed that BRAF interacts and phosphorylates PAX3. PMID: 27906130
    13. Using a conditional allele for Braf(V600E) , a mutation observed in clinical cases of GIST, authors observed that Braf(V600E) activation was sufficient to drive ICC hyperplasia but not GIST tumorigenesis. PMID: 28539323
    14. This study detected the BrafV637E mutation by whole-exome analysis in 4/4 hepatic tumors induced by neonatal treatment with diethylnitrosamine (DEN) in male B6C3F1 mice. PMID: 27253992
    15. a critical threshold for inhibition of MAPK signaling is required to optimally restore expression of thyroid differentiation genes in thyroid cells and in mice with BrafV600E-induced thyroid cancer. PMID: 27669459
    16. A- and B-Raf ablation in chondrocytes does not alter skeletal development, whereas ablation of C-Raf decreases hypertrophic chondrocyte apoptosis and impairs vascularization of the growth plate. However, ablation of C-Raf does not impair phosphate-induced ERK1/2 phosphorylation in vitro, but leads to rickets by decreasing VEGF protein stability. PMID: 28073913
    17. these contrasting signatures precisely match those proposed to confer bias toward Hras(CAA61CTA) versus Braf(GTG636GAG) mutations in the original tumor sets. Our findings highlight a novel mechanism whereby exposure history acts through strand-biased mutagenesis to specify activation of preferred oncogenes PMID: 27207659
    18. these results suggest that the activation of 5-HT1D receptors selectively enhanced IA via the Gbetagamma of the Go-protein, PKA, and the sequential B-Raf-dependent p38 MAPK signaling cascade. PMID: 27156838
    19. BRAF V600E inhibition stimulates AMP-activated protein kinase-mediated autophagy in colorectal cancer cells. PMID: 26750638
    20. these data confirm the existence of a negative feedback pathway by which BRAF protein stability is regulated by ERK. PMID: 26898828
    21. coexpression of BRAF(V600E) and KRAS(G12D) in early tumorigenesis leads to negative selection due to oncogene-induced senescence PMID: 26028035
    22. findings suggest that targeting ErbB-3 receptors could represent an effective therapeutic approach in BRAF-V600E mutant colon cancer PMID: 26160848
    23. Cotargeting is the treatment for mutant RAF/MEK/ERK and NF-kB pathways to treat mutant BRAF melanomas. PMID: 25751672
    24. The CM cell lines, as well as the tumor xenografts and their metastases, were positive for the melanoma markers HMB-45, S100B, and MART-1. Two cell lines and their corresponding xenografts carried a BRAF mutation, the third showed an NRAS mutation. PMID: 25722211
    25. New BRAF knockin mice provide a pathogenetic mechanism of developmental defects and a therapeutic approach in cardiofaciocutaneous syndrome. PMID: 25035421
    26. Results demonstrate that BRAF activates Usp5 to suppress cell cycle checkpoint control and apoptosis by blocking p53 and FAS induction. PMID: 24980819
    27. The implantation of genetically defined murine BRAF-mutated thyroid cancer cell lines into syngeneic mice results in rapid and synchronous tumor formation. PMID: 24295207
    28. early activation of the RAF signaling pathway in the ectoderm has effects on specific steps of epidermal differentiation. PMID: 25202828
    29. The study characterizes a mouse model of melanogenesis where simultaneous Cdkn2a and Lkb1 inactivation in Braf(V600E) melanocytes results in activation of both mTORC1 and mTORC2/Akt, inducing rapid melanoma formation in mice. PMID: 25584893
    30. The B-Raf(V600E) inhibitor dabrafenib selectively inhibits RIP3 and alleviates acetaminophen-induced liver injury. PMID: 24901049
    31. Overexpression of BRAF(V600E) in normal thyroid epithelial (H tori) cells also reduced the effects of Dkk-1 on cell survival. PMID: 24848709
    32. Inhibition of BRAF induces metastasis in RAS mutant or inhibitor-resistant melanoma cells by reactivating MEK and ERK signaling. PMID: 24667377
    33. MYC inactivation restores BRAF(V600E)-induced senescence in lung tumors in mice. PMID: 24934810
    34. these data indicate that B-RAF is an important factor in oncogenic C-RAF-mediated tumorigenesis. PMID: 25096573
    35. Braf(V600E)-driven tumors become addicted to autophagy as a means to preserve mitochondrial function and glutamine metabolism PMID: 24362353
    36. COX-2 inhibition prevents the appearance of cutaneous squamous cell carcinomas accelerated by BRAF inhibitors PMID: 24345644
    37. in mice, mutant Trp53 accelerated BRAF(V600E)-driven melanomagenesis--and TP53 mutations are linked to evidence of UVR-induced DNA damage in human melanoma PMID: 24919155
    38. results provide evidence that constitutively activated BRAF(V600E) drives aberrant proliferation of monocyte-lineage cells. PMID: 24152792
    39. Cell-intrinsic RAF signaling is a crucial pathway promoting developmental and regenerative axon growth in the peripheral and central nervous systems. PMID: 24733831
    40. p53 constrains progression from papillary thyroid cancer to anaplastic thyroid carcinoma in a Braf-mutant mouse. PMID: 24711431
    41. decreasing the levels of CTR1 (Cu transporter 1), or mutations in MEK1 that disrupt Cu binding, decreased BRAF(V600E)-driven signalling and tumorigenesis in mice and human cell settings PMID: 24717435
    42. constitutive BRAF pathway activation in BRAF(Nav1.8) mice results in ectopic and enhanced expression of itch-sensing genes, including gastrin-releasing peptide and MAS-related GPCR member A3, in nociceptors expressing TRPV1 PMID: 24216512
    43. LKB1 haploinsufficiency as a risk factor for tumor progression of BRAF(V600E) mutated lung adenomas in human cancer patients PMID: 23825589
    44. EGF, which signals through CRAF, and an activated BRAF mutant also activate PKC and stimulate cell migration through up-regulating RFFL expression. PMID: 24114843
    45. BRAF(V600E) and ARF deletion synergize to inhibit nucleotide excision repair by epigenetically repressing XPC. PMID: 23650282
    46. These results suggest that BRAF(V600E) does not appear to induce papillary thyroid carcinomas-like lesions when expressed in a fraction of thyroid cells postnatally under normal TSH concentrations. PMID: 23970782
    47. The loss of B-Raf significantly reduces MAPK activation in wild type MEFs. PMID: 23416466
    48. ERK1/2 activation in response to CRH is biphasic, involving a first cAMP- and B-Raf-dependent early phase and a second phase that critically depends on CRHR1 internalization and beta-arrestin2. PMID: 23371389
    49. role in survival of double-positive thymocytes and in functional activity of single-positive T cells in the periphery PMID: 23334952
    50. in the absence of BRaf all neuronal cellular structures develop, but neuronal circuits in the cerebellum and hippocampus are partially disturbed besides impaired neuronal generation in both structures. PMID: 23505473

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  • 相關(guān)疾?。?/div>
    Participates in a chromosomal translocation that produces a Tif1a-BRAF (T18) oncogene originally isolated from a furfural-induced hepatoma.
  • 亞細胞定位：
    Nucleus. Cytoplasm. Cell membrane.
  • 蛋白家族：
    Protein kinase superfamily, TKL Ser/Thr protein kinase family, RAF subfamily
  • 數(shù)據(jù)庫鏈接：