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  4. Recombinant Mouse Syntaxin-binding protein 1 (Stxbp1)

Recombinant Mouse Syntaxin-binding protein 1 (Stxbp1)

  • 中文名稱：
    小鼠Stxbp1重組蛋白
  • 貨號(hào)：
    CSB-YP022905MO
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Stxbp1重組蛋白
  • 貨號(hào)：
    CSB-EP022905MO
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Stxbp1重組蛋白
  • 貨號(hào)：
    CSB-EP022905MO-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Stxbp1重組蛋白
  • 貨號(hào)：
    CSB-BP022905MO
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Stxbp1重組蛋白
  • 貨號(hào)：
    CSB-MP022905MO
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    Stxbp1Syntaxin-binding protein 1; Protein unc-18 homolog 1; Unc18-1; Protein unc-18 homolog A; Unc-18A
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長(zhǎng)度：
    Full length protein
  • 表達(dá)區(qū)域：
    1-594
  • 氨基酸序列
    MAPIGLKAVV GEKIMHDVIK KVKKKGEWKV LVVDQLSMRM LSSCCKMTDI MTEGITIVED INKRREPLPS LEAVYLITPS EKSVHSLISD FKDPPTAKYR AAHVFFTDSC PDALFNELVK SRAAKVIKTL TEINIAFLPY ESQVYSLDSA DSFQSFYSPH KAQMKNPILE RLAEQIATLC ATLKEYPAVR YRGEYKDNAL LAQLIQDKLD AYKADDPTMG EGPDKARSQL LILDRGFDPS SPVLHELTFQ AMSYDLLPIE NDVYKYETSG IGEARVKEVL LDEDDDLWIA LRHKHIAEVS QEVTRSLKDF SSSKRMNTGE KTTMRDLSQM LKKMPQYQKE LSKYSTHLHL AEDCMKHYQG TVDKLCRVEQ DLAMGTDAEG EKIKDPMRAI VPILLDANVS TYDKIRIILL YIFLKNGITE ENLNKLIQHA QIPPEDSEII TNMAHLGVPI VTDSTLRRRS KPERKERISE QTYQLSRWTP IIKDIMEDTI EDKLDTKHYP YISTRSSASF STTAVSARYG HWHKNKAPGE YRSGPRLIIF ILGGVSLNEM RCAYEVTQAN GKWEVLIGST HILTPQKLLD TLKKLNKTDE EISS
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Participates in the regulation of synaptic vesicle docking and fusion through interaction with GTP-binding proteins. Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions.
  • 基因功能參考文獻(xiàn)：
    1. SFK-dependent Munc18-1 phosphorylation may constitute a potent, previously unknown mechanism to shut down synaptic transmission, via direct occlusion of a Synaptobrevin/VAMP2 binding groove and subsequent hindrance of conformational changes in domain 3a responsible for vesicle priming. PMID: 29150433
    2. This study demonstrated that the disruption of Munc18-1 function may result in the abnormal corticogenesis, leading to neurodevelopmental disorders with MUNC18-1 gene abnormalities. PMID: 29191246
    3. Findings suggest that synaptic impairments of the dorsal telencephalic and subcortical excitatory neurons cause learning deficits and enhanced aggression in Stxbp1+/- mice, respectively. PMID: 29040524
    4. This results of study concluded that a conformational change within helix 12 is responsible for the essential postdocking role of Munc18-1 in neurosecretion. PMID: 27358447
    5. M18L was localized to presynaptic inhibitory terminals, and was associated with cognitive function and protection from dementia in an elderly PMID: 26628003
    6. In this studying the Munc18-1-/- nervous system, we demonstrate that synaptic activity is dispensable for the early formation of spinal motor circuits at the levels of axon guidance, differentiation of transcriptional identity, and mRNA expression. PMID: 26758845
    7. Vamp2 mutations that impair Munc18-1 binding inhibit spontaneous as well as evoked neurotransmitter release, providing evidence for the Vamp2-regulating function of Munc18-1 in synaptic exocytosis. PMID: 26572858
    8. A dynamic PKC phosphorylation/de-phosphorylation cycle of Munc18-1 drives short-term enhancement of transmitter release during post-tetanic potentiation. PMID: 24520164
    9. miR-218 and miR-322 directly interact with Stxbp1 by targeting the 3'UTR of its mRNA. PMID: 25489007
    10. Heart rate results indicate enhanced conditioned fear in munc-18-1HZ mice PMID: 24304718
    11. Munc18-1 levels correlate with synaptic strength. PMID: 24590174
    12. Syntaxin binding protein 1 is not required for allergic inflammation via IgE-mediated mast cell activation. PMID: 23484036
    13. [review] Regulation of cortical F-actin is a shared function of Sec1/Munc18-like proteins; a way to gain more insight in the molecular mechanism underlying the Munc18-1-mediated cortical F-actin regulation is proposed. PMID: 22535313
    14. SNAREpin assembly by Munc18-1 requires previous vesicle docking by synaptotagmin 1. PMID: 22810233
    15. a novel indirect role for Munc18-1 in facilitating syntaxin 4-mediated granule pre-docking to support first-phase insulin exocytosis. PMID: 22685295
    16. Munc18-1 and Munc18-2 display distinct subcellular compartmentalization and can coordinate the insulin exocytotic process differently as a consequence of the actual [Ca(2+)](i). PMID: 21690086
    17. Dletion of Munc18-1 in 5-HT (serotonin) results in rapid degeneration of the 5-HT system. PMID: 22140524
    18. Munc18, but not Munc13, regulates growth cone filopodia, potentially via its previously observed effect on filamentous actin. PMID: 20497124
    19. Munc18-1 activates a SNARE complex with the typical configuration, in which each of the SNARE core domains is individually rooted in the membrane bilayer. PMID: 20603329
    20. in the absence of presynaptic secretion immature synapses with a normal morphology form, but fewer in number in munc18-1 deficient mice PMID: 15145078
    21. Morphological and immunohistological analysis of the hypothalamo-neurohypophysial system in munc18-1 nul mutant embryos during brain development showed that this peptidergic system was formed normally during early embryogenesis. PMID: 15182301
    22. The lack of synaptic activity cannot explain the degeneration in munc18-1-null mutants. Second, trophic support delays but cannot prevent degeneration. PMID: 15255974
    23. this study provides supporting evidence for the involvement of Stxbp1 in ethanol preference drinking PMID: 15897714
    24. Munc18-1 is involved in the secretion of peptide hormones and in the docking of large dense-core vesicles PMID: 15898951
    25. Munc18-1 either controls the spatially correct assembly of core complexes for SNARE-dependent fusion, or acts as a direct component of the fusion machinery itself. PMID: 15935055
    26. Docking vesicles either undock immediately or are captured by minimal tethering machinery and converted in a munc18-1/syntaxin-dependent, strongly tethered, fusion-competent state. PMID: 16902411
    27. number of vesicles docked at the active zone and the total number of vesicles per terminal correlated with both munc18-1 expression levels and the size of the releasable vesicle pool PMID: 17110441
    28. Munc18: a presynaptic transmitter release site N type (CaV2.2) calcium channel interacting protein. PMID: 19170253
    29. Munc18-1 binding to SNARE complexes mediates synaptic vesicle priming; the resulting primed state involves a Munc18-1-SNARE-complexin macromolecular assembly that is poised for Ca(2+) triggering of fusion. PMID: 19255244
    30. show that presynaptic metabotropic glutamate receptors (mGluRs) bind and release Munc18-1 in a Ca(2+)-dependent manner, whose actions decrease and increase synaptic vesicle release, respectively. PMID: 19822743

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  • 亞細(xì)胞定位：
    Cytoplasm, cytosol. Membrane; Peripheral membrane protein.
  • 蛋白家族：
    STXBP/unc-18/SEC1 family
  • 組織特異性：
    Expressed in the inner and outer plexiform layers of the retina (at protein level).
  • 數(shù)據(jù)庫(kù)鏈接：