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  4. Recombinant Mouse Transcriptional regulator ATRX (Atrx), partial

Recombinant Mouse Transcriptional regulator ATRX (Atrx), partial

  • 中文名稱：
    Recombinant Mouse Transcriptional regulator ATRX(Atrx) ,partial,Yeast
  • 貨號(hào)：
    CSB-YP726755MO
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Mouse Transcriptional regulator ATRX(Atrx) ,partial,Yeast
  • 貨號(hào)：
    CSB-EP726755MO
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Mouse Transcriptional regulator ATRX(Atrx) ,partial,Yeast
  • 貨號(hào)：
    CSB-EP726755MO-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Mouse Transcriptional regulator ATRX(Atrx) ,partial,Yeast
  • 貨號(hào)：
    CSB-BP726755MO
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Mouse Transcriptional regulator ATRX(Atrx) ,partial,Yeast
  • 貨號(hào)：
    CSB-MP726755MO
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Atrx
  • Uniprot No.：
  • 別名：
    Atrx; Hp1bp2; XnpTranscriptional regulator ATRX; EC 3.6.4.12; ATP-dependent helicase ATRX; HP1 alpha-interacting protein; HP1-BP38 protein; Heterochromatin protein 2; X-linked nuclear protein
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長(zhǎng)度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Involved in transcriptional regulation and chromatin remodeling. Facilitates DNA replication in multiple cellular environments and is required for efficient replication of a subset of genomic loci. Binds to DNA tandem repeat sequences in both telomeres and euchromatin and in vitro binds DNA quadruplex structures. May help stabilizing G-rich regions into regular chromatin structures by remodeling G4 DNA and incorporating H3.3-containing nucleosomes. Catalytic component of the chromatin remodeling complex ATRX:DAXX which has ATP-dependent DNA translocase activity and catalyzes the replication-independent deposition of histone H3.3 in pericentric DNA repeats outside S-phase and telomeres, and the in vitro remodeling of H3.3-containing nucleosomes. Its heterochromatin targeting is proposed to involve a combinatorial readout of histone H3 modifications (specifically methylation states of H3K9 and H3K4) and association with CBX5. Involved in maintaining telomere structural integrity in embryonic stem cells probably implying recruitment of CBX5 to telomeres. Reports on the involvement in transcriptional regulation of telomeric repeat-containing RNA (TERRA) are conflicting; according is required for its transcriptional repression in embryonic stem cells. Acts as negative regulator of chromatin incorporation of transcriptionally repressive histone MACROH2A1, particularily at telomeres. Participates in the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, required for the chromatin occupancy of SMC1 and CTCTF within the H19 imprinting control region (ICR) and involved in esatblishment of histone tails modifications in the ICR. Binds to zinc-finger coding genes with atypical chromatin signatures and regulates its H3K9me3 levels. Forms a complex with ZNF274, TRIM28 and SETDB1 to facilitate the deposition and maintenance of H3K9me3 at the 3' exons of zinc-finger genes.
  • 基因功能參考文獻(xiàn)：
    1. Atrx-dependent gene expression changes in neuroepithelial progenitor cells were accompanied by altered cellular morphology, increased motility, and a shift in differentiation markers toward an astrocytic histiogenic profile. These latter two phenotypes recapitulate two cardinal features of ATRX-mutant gliomas. PMID: 29535300
    2. PML protein organizes heterochromatin domains where it regulates histone H3.3 deposition by ATRX and DAXX. PMID: 28341773
    3. Gene expression changes in the Atrx-null retina indicate defective synaptic structure and neuronal circuitry, suggest excitotoxic mechanisms of neurodegeneration, and demonstrate that common targets of ATRX in the forebrain and retina may contribute to similar neuropathological processes underlying cognitive impairment and visual dysfunction in ATR-X syndrome. PMID: 28173139
    4. mosaic loss of ATRX expression in the central nervous system leads to endocrine defects and decreased body size and has a negative impact on learning and memory. PMID: 28093507
    5. The results suggest that ATRX is required to limit replication stress during cellular proliferation, whereas upregulation of PARP-1 activity functions as a compensatory mechanism to protect stalled forks, limiting genomic damage, and facilitating late-born neuron production. PMID: 27171262
    6. The long noncoding RNA, TERRA can bind both in cis to telomeres and in trans to genic targets; a large network of interacting proteins was defined, including epigenetic factors, telomeric proteins, and the RNA helicase, ATRX. TERRA and ATRX share hundreds of target genes and are functionally antagonistic at these loci: whereas TERRA activates, ATRX represses gene expression. PMID: 28666128
    7. The changes of ATRX distribution occur and partially correlate with the main stages of zygotic genome activation during mouse early development, butthese changes seem to be determined by other processes of structural and functional rearrangements of blastomere nuclei. PMID: 27863708
    8. ATRX mutation is associated with increased mutation rate at the single-nucleotide variant (SNV) level. PMID: 26936505
    9. Daxx and Atrx safeguard the genome by silencing repetitive elements when DNA methylation levels are low. PMID: 26340527
    10. A direct role of Atrx in the establishment and robust maintenance of heterochromatin is demonstrated. PMID: 26012739
    11. We propose a model whereby ATRX-dependent deposition of H3.3 into heterochromatin is normally required to maintain the memory of silencing at imprinted loci. PMID: 25865896
    12. ATRX promotes the incorporation of histone H3.3 at particular transcribed genes and facilitates transcriptional elongation through G-rich sequences. PMID: 25452430
    13. Using X chromosome inactivation as a model, study applied an unbiased proteomics approach to isolate Xist and PRC2 regulators and identified ATRX; ATRX functions as a high-affinity RNA-binding protein that directly interacts with RepA/Xist RNA to promote loading of PRC2 in vivo. Without ATRX, PRC2 cannot load onto Xist RNA nor spread in cis along the X chromosome. PMID: 25417162
    14. These results indicate that while ATRX plays limited roles during early stages of skeletal development, deficiency of the protein in adult tissues does not confer susceptibility to osteoarthritis. PMID: 24386478
    15. ATRX plays a direct role in facilitating DNA replication. Ablation of ATRX alone, although leading to a DNA damage response at telomeres, is not sufficient to trigger the alternative lengthening of telomere pathway in mouse embryonic stem cells. PMID: 24651726
    16. The findings indicate that ATRX regulates gene expression at a subset of imprinted domains by maintaining a nucleosome configuration conducive to CTCF binding and to the maintenance of higher order chromatin structure. PMID: 24990380
    17. Targeted loss of the ATR-X syndrome protein in the limb mesenchyme of mice causes brachydactyly. PMID: 23892236
    18. loss of ATRX increases DNA damage locally in the forebrain and anterior pituitary and causes tissue attrition and other systemic defects similar to those seen in aging PMID: 23563309
    19. Results suggest that cells lacking Atrx are more sensitive to DNA damaging agents and that this may result in enhanced death during development when cells are at their proliferative peak. PMID: 23284920
    20. Atrx maintains chromatin integrity during the rapid developmental growth of a tissue. PMID: 23114596
    21. The presence of centromeric breaks during the transition to the first mitosis in the early embryo indicates that the role of ATRX in chromosome segregation is mediated through an epigenetic mechanism involving the maintenance of chromatin modifications PMID: 22918800
    22. Reduced expression of the ATRX gene, a chromatin-remodeling factor, causes hippocampal dysfunction in mice PMID: 20865721
    23. Data suggest that ATRX has a critical and conserved role in normal development of the testis and ovary in both the somatic and germ cells. PMID: 21672208
    24. Disruption of H3K9me3 binding may be a general pathogenicity pathway of ATRX mutations in the ADD domain. PMID: 21421568
    25. ATRX and the androgen receptor (AR) physically interact in the testis and in the Sertoli cell line TM4 and co-operatively activate the promoter of Rhox5. PMID: 21427128
    26. ATRX is required for centromere stability and the epigenetic control of heterochromatin function during meiosis and the transition to the first mitosis. PMID: 20885787
    27. Study shows that ATRX binds G4 DNA in vitro, suggesting a common mechanism by which ATRX may influence a wide range of nuclear processes in the telomeric, subtelomeric, and interstitial regions of mammalian chromosomes. PMID: 21029860
    28. Daxx functions as an H3.3-specific chaperone and facilitates the deposition of H3.3 at heterochromatin loci in the context of the ATRX-Daxx complex PMID: 20651253
    29. ATRX loss of function alters enrichment of cohesin, CTCF, and histone modifications at the H19 ICR, without affecting DNA methylation on the paternal allele. PMID: 20159591
    30. ATRX is not directly required for development or growth of cartilage in the mouse PMID: 19774083
    31. centromeric ATRX is required for correct chromosome alignment and organization of a bipolar meiotic metaphase II spindle. PMID: 15242786
    32. Daxx and SWI/SNF protein ATRX are regulated by heterochromatin and ND10 during the cell cycle PMID: 15252119
    33. ATRX is a critical mediator of cell survival during early neuronal differentiation. PMID: 15668733
    34. Atrx has a role in the development of the murine trophoblast PMID: 16628246
    35. Disruption of the MeCP2-ATRX interaction leads to pathological changes that contribute to mental retardation. PMID: 17296936
    36. In fewmale mice heterozygous for a null ATRX allele, x-chromosome inactivation is balanced early in embryogenesis but becomes skewed over the course of development, because of selection favoring cells expressing the wild-type Atrx allele. PMID: 17503331
    37. Study demonstrates that loss of ATRX in the embryonic mouse brain induces mitotic defects in neuroprogenitors in vivo with evidence of abnormal chromosome congression and segregation. PMID: 18227278
    38. ATRX as well as H3K9me3 associate with Y chromosome-specific DNA sequences and decorate both arms of Y chromosome, suggesting possible role in heterochromatinization and predominant transcriptional quiescence of this chromosome during spermatogenesis. PMID: 18366812
    39. ATRX controls the expression of ancestral PAR1 genes that have translocated to autosomes in the mouse. PMID: 18842153
    40. in somatic and trophoblast stem cells, ATRX exhibits a specific association with sequences located within the previously described H3K9me2-hotspot, a region 5' to the X inactive-specific transcript (Xist) locus PMID: 19005673
    41. Atrx plays role in interneuron survival and differentiation. PMID: 19088125

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  • 亞細(xì)胞定位：
    Nucleus. Chromosome, telomere. Nucleus, PML body.
  • 蛋白家族：
    SNF2/RAD54 helicase family
  • 數(shù)據(jù)庫鏈接：