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  4. Recombinant Mouse Transient receptor potential cation channel subfamily M member 4 (Trpm4), partial

Recombinant Mouse Transient receptor potential cation channel subfamily M member 4 (Trpm4), partial

  • 中文名稱：
    小鼠Trpm4重組蛋白
  • 貨號：
    CSB-YP763162MO
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Trpm4重組蛋白
  • 貨號：
    CSB-EP763162MO
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Trpm4重組蛋白
  • 貨號：
    CSB-EP763162MO-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Trpm4重組蛋白
  • 貨號：
    CSB-BP763162MO
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Trpm4重組蛋白
  • 貨號：
    CSB-MP763162MO
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Trpm4
  • Uniprot No.：
  • 別名：
    Trpm4; Ltrpc4; Transient receptor potential cation channel subfamily M member 4; Calcium-activated non-selective cation channel 1; Long transient receptor potential channel 4; LTrpC-4; LTrpC4
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Calcium-activated non selective (CAN) cation channel that mediates membrane depolarization. While it is activated by increase in intracellular Ca(2+), it is impermeable to it. Mediates transport of monovalent cations (Na(+) > K(+) > Cs(+) > Li(+)), leading to depolarize the membrane. It thereby plays a central role in cadiomyocytes, neurons from entorhinal cortex, dorsal root and vomeronasal neurons, endocrine pancreas cells, kidney epithelial cells, cochlea hair cells etc. Participates in T-cell activation by modulating Ca(2+) oscillations after T lymphocyte activation, which is required for NFAT-dependent IL2 production. Involved in myogenic constriction of cerebral arteries. Controls insulin secretion in pancreatic beta-cells. May also be involved in pacemaking or could cause irregular electrical activity under conditions of Ca(2+) overload. Affects T-helper 1 (Th1) and T-helper 2 (Th2) cell motility and cytokine production through differential regulation of calcium signaling and NFATC1 localization. Enhances cell proliferation through up-regulation of the beta-catenin signaling pathway. Essential for the migration but not the maturation of dendritic cells. Plays a role in keratinocyte differentiation.
  • 基因功能參考文獻：
    1. The activation of TRPM4 during ischemia-reperfusion injury involves the increase in both, intracellular calcium and H2O2, which may act together to produce a sustained activation of the channel. PMID: 28876976
    2. Both TRPM4 and TRPM5 are required for normal responses to bitter, sweet, and umami stimuli. PMID: 29311301
    3. The findings of this study demonstrated a novel molecular mechanism involving the SUR1-TRPM4-AQP4 complex to account for bulk water influx during astrocyte swelling. PMID: 28906027
    4. Calcium studies demonstrated that TRPM4 channel negatively regulates calcium entry providing support for activation of the Cn-NFAT pathway in Trpm4 (-/-) mice. Authors provide evidence for the functional expression of TRPM4 channel in response to endurance training. PMID: 28224334
    5. Study demonstrates that TRPM4 functions as a limiting factor for antigen evoked calcium rise in connective tissue type mast cells and concurrent translocation of TRPM4 into the plasma membrane is part of this mechanism. PMID: 27624684
    6. Atrial TRPM4 channel is activated by a physiological range of Ca2+ concentrations and its excessive activity can cause arrhythmic changes. PMID: 28898995
    7. electron cryo-microscopy structures of the mouse TRPM4 channel with and without ATP PMID: 29211714
    8. Disrupting the Trpm4 gene in mice specifically eliminates NMDAR-dependent LTP. PMID: 26631168
    9. observations are consistent with a model in which TRPM4 is a regulator of calcium homeostasis in cardiomyocytes after AngII stimulation PMID: 26043922
    10. The PKC-dependent effect of GLP-1 on membrane potential and electrical activity was mediated by activation of Na(+)-permeable TRPM4 and TRPM5 channels by mobilization of intracellular Ca(2+) from thapsigargin-sensitive Ca(2+) stores PMID: 26571400
    11. The function of TRPM4 in renal primary cilia is not yet known, but it is likely to influence the apical Ca(2) dynamics of the cell PMID: 26290373
    12. Deletion of the Trpm4 gene in mice improved survival and significantly enhanced beta-adrenergic cardiac reserve after inducing ischaemic heart failure. PMID: 25600961
    13. TRPM4 has pleiotropic roles in the heart, including the regulation of conduction and cellular electrical activity which impact heart development. PMID: 25531103
    14. The present study demonstrates that robust TRPM4-IR is localized specifically in the soma of Inner Auditory Hair Cells in the organ of Corti. PMID: 24840118
    15. These results showed that the cell surface expression of TRPM4 channels is mediated by 14-3-3gamma binding. PMID: 25047048
    16. Adenylyl cyclase-mediated effects contribute to increased isoprenaline-induced cardiac contractility in TRPM4-deficient mice. PMID: 24972051
    17. Results show that functional TRPM4 proteins are novel determinants of the inotropic effect of beta-adrenergic stimulation on the ventricular heart muscle. PMID: 24226423
    18. N-Glycosylation is not required for surface expression, but complex N-glycosylation stabilizes Trpm4b surface expression. PMID: 24214984
    19. The results showed that TRPM4 is implicated in the waveform of the atrial action potential PMID: 23416167
    20. The results of this study suggested that TRPC4, TRPM4, TRPM8 and TRPV1, showed statistically significant variation in mRNA levels between DRGs from different segments, suggesting ganglion-specific regulation of TRP channel gene expression. PMID: 23410158
    21. Experiments with FRET and co-immunoprecipitation showed de novo appearance of Sur1-Trpm4 heteromers after spinal cord injury in rats. PMID: 23255597
    22. The TRPM4 channel contributes to survival in septic peritonitis; monocytes and macrophages (but not neutrophils) confer a survival advantage during sepsis through their expression of Trpm4. PMID: 22933633
    23. SUR1 controls K(ATP) channel activity but not TRPM4 channels. PMID: 22291026
    24. cell membrane depolarization by TRPM4 may play an important role in controlling glucagon secretion from alpha-cells and perhaps glucose homeostasis PMID: 21238535
    25. discussion of data from various lines of investigation on the role of TRPM4 and TRPM5 in glucose-stimulated insulin secretion in pancreatic islets PMID: 21099334
    26. We therefore conclude that TRPM4 proteins limit catecholamine release from chromaffin cells and that this contributes to increased sympathetic tone and hypertension. PMID: 20679729
    27. Inhibition of Trpm4 expression increases divalent calcium ion influx and oscillatory calcium signaling levels in T helper (Th)2 cells and decreases influx and oscillations in Th1 cells. PMID: 20656926
    28. PKCdelta activity causes smooth muscle depolarization and vasoconstriction by increasing the number of TRPM4 channels in the sarcolemma. PMID: 20610768
    29. Voltage dependence is not due to block by divalent cations or to voltage-dependent binding of intracellular Ca2+ to an activator site, indicating that TRPM4 is a transient receptor potential channel with an intrinsic voltage-sensing mechanism. PMID: 12799367
    30. Molecular cloning and characterization of TRPM4. PMID: 12893253
    31. TRPM5 activated at lower Ca2+ concentration than TRPM4 when [Ca2+]i was raised. Different Ca2+ concentrations gave a difference in Ca2+ sensitivity between TRPM4 and TRPM5, with EC50 values of 20.2+/-4.0 microM & 0.70+/-0.1 microM, respectively. PMID: 15670874
    32. hydrolysis of PI(4,5)P(2) underlies desensitization of TRPM4, and PI(4,5)P(2) is a general regulator for the gating of TRPM ion channels PMID: 16186107
    33. TRPM4-regulated Ca(2+) homeostasis is crucial for DC mobility but not maturation PMID: 18758465
    34. TRPM4 is critically involved in migration of bone marrow-derived mast cells by regulation of Ca(2+)-dependent actin cytoskeleton rearrangements. PMID: 19046767
    35. Depolarizing currents generated by TRPM4 are an important component in the control of intracellular Ca(2+) signals necessary for insulin secretion and perhaps glucagon from alpha-cells. PMID: 19063936
    36. in vivo gene suppression in rats treated with Trpm4 antisense or in Trpm4(-/-) mice preserved capillary structural integrity, eliminated secondary hemorrhage, yielded a 3x to 5x reduction in lesion volume and produced improvement in neurological function PMID: 19169264

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  • 亞細胞定位：
    Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum. Golgi apparatus.
  • 蛋白家族：
    Transient receptor (TC 1.A.4) family, LTrpC subfamily, TRPM4 sub-subfamily
  • 組織特異性：
    Sino-atrial nodes (at protein level). Widely expressed.
  • 數(shù)據庫鏈接：