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  4. Recombinant Mouse Transient receptor potential cation channel subfamily M member 7 (Trpm7), partial

Recombinant Mouse Transient receptor potential cation channel subfamily M member 7 (Trpm7), partial

  • 中文名稱：
    小鼠Trpm7重組蛋白
  • 貨號：
    CSB-YP856384MO
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Trpm7重組蛋白
  • 貨號：
    CSB-EP856384MO
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Trpm7重組蛋白
  • 貨號：
    CSB-EP856384MO-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Trpm7重組蛋白
  • 貨號：
    CSB-BP856384MO
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Trpm7重組蛋白
  • 貨號：
    CSB-MP856384MO
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    Trpm7; Chak; Ltrpc7; Transient receptor potential cation channel subfamily M member 7; EC 2.7.11.1; Channel-kinase 1; Long transient receptor potential channel 7; LTrpC-7; LTrpC7; Transient receptor potential-phospholipase C-interacting kinase; TRP-PLIK
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Essential ion channel and serine/threonine-protein kinase. Divalent cation channel permeable to calcium and magnesium. Has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death. Involved in TNF-induced necroptosis downstream of MLKL by mediating calcium influx. The kinase activity is essential for the channel function. May be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell. Phosphorylates annexin A1 (ANXA1).
  • 基因功能參考文獻(xiàn)：
    1. TRPM7/miR-34a gene silencing could improve spatial cognitive function and protect hippocampal neurogenesis in mice with T1DM. PMID: 28185128
    2. The enzymatic activity of TRPM7 is not essential for thymopoiesis, but is required for CD103 transcription and gut-homing of intra-epithelial lymphocytes. Mechanistically, TRPM7 kinase activity controls TGF-beta-induced CD103 expression and pro-inflammatory T helper 17, but not regulatory T, cell differentiation by modulating SMAD2. PMID: 29203869
    3. results provide a structural framework for understanding the TRPM1/3/6/7 subfamily and extend the knowledge base upon which to study the diversity and evolution of TRP channels. PMID: 30108148
    4. Transient receptor potential melastatin-like 7, a non-selective but Ca(2+)-conducting ion channel, mediates the cytosolic Ca(2+) elevations essential for lipopolysaccharide-induced macrophage activation PMID: 29343440
    5. The present results suggested that TRPM7 may be involved in Ca2+/Mg2+ homeostasis during chondrocyte hypertrophy, and contribute to endochondral ossification via interacting with the PI3KAkt signaling pathway. PMID: 28849029
    6. TRPM7 kinase activity regulates mast cell degranulation by changing its sensitivity to intracellular calcium. PMID: 26660477
    7. TRPM7 is part of a large cytoskeletal complex that may affect the malignant potential of tumor cells by regulating actomyosin dynamics and cell-matrix interactions. PMID: 27402209
    8. Inhibition of TRPM7 reduces calcium influx and accelerates the polarization of hippocampal neurons. PMID: 25502295
    9. our results suggest the differential roles of TRPM7 in endochondral and intramembranous ossification. PMID: 26558702
    10. TRPM7 dysfunction may cause macrothrombocytopenia in humans and mice. PMID: 27020697
    11. TRPM7 kinase is a novel player in Angiotensin II induced hypertension and vascular and target organ damage. PMID: 26928801
    12. Immature intercellular junctions in Trpm7 knock-out mice might lead to a disruption of barrier function resulting in inflammation and hypersensitive bladder afferent nerves that may affect voiding behavior in vivo PMID: 26504086
    13. By inhibiting TRPM7 and ANO1 via opioid receptor signaling pathways. PMID: 25967872
    14. Abolishing TRPM7 kinase activity does not impair its channel activity and kinase activity is not essential for regulation of mammalian Mg(2+) homeostasis. PMID: 25030553
    15. results suggest that TRPM7 kinase is a sensor of magnesium status and provides coordination of cellular and systemic responses to magnesium deprivation. PMID: 25534891
    16. these data suggest that oxidative stress inhibits TRPM7 current under pathological conditions that accompany intracellular ATP depletion and free [Mg(2+)]i elevation. PMID: 24747489
    17. Study shows that in normal tissues and cell lines, the kinase is proteolytically cleaved from the channel domain in a cell-type-specific manner. These TRPM7 cleaved kinase fragments (M7CKs) translocate to the nucleus and bind multiple components of chromatin-remodeling complexes, including Polycomb group proteins. PMID: 24855944
    18. TRPM7 affects actomyosin contractility and invadosome formation independent of Ca2+ influx. PMID: 24176224
    19. The present study demonstrates for the first time that TRPM7 channels regulate cell cycle and adipogenesis of 3T3-L1 preadipocytes. PMID: 23765921
    20. Data suggest that TRPM7 influences diastolic membrane depolarization and automaticity in embryonic myocardium and sinoatrial node (SAN) indirectly via regulation of Hcn4 expression. PMID: 23878236
    21. Loss of Trpm7 at an intermediate development time point alters myocardial transcriptional profile in adulthood. PMID: 23734001
    22. upregulation of TRPM7 channels by Ang II contributes to the development of the proliferative phenotype of ascending aortic vascular smooth muscle cells. PMID: 22896586
    23. depletion of TRPM7 by RNA interference in fibroblasts increases cell resistance to apoptotic stimuli by decreasing ROS levels in an Mg(2+)-dependent manner PMID: 22587440
    24. It was shown that TRPM7 regulates endocytic compartmentalization of the Fas receptor after receptor stimulation, an important process for apoptotic signaling. PMID: 22698280
    25. The present results show that TRPM7 channels are phosphorylated at multiple sites, which serves as a mechanism to modulate the dynamic functions of TRPM7 channels in mammalian cells. PMID: 22222377
    26. Trpm7 disruption prevents the formation of the NS cell monolayer. The in vivo and in vitro results demonstrate a temporal requirement for the Trpm7 channel kinase during embryogenesis PMID: 22203997
    27. in a model of inherited hypomagnesemia, TRPM6 and TRPM7, but not paracellin-1, are downregulated PMID: 21602712
    28. Findings indicate that H(2)O(2) can induce Ca(2+) toxicity independent of glutamate receptors and voltage-gated Ca(2+) channels. Activation of TRPM7 channels is involved in such toxicity. PMID: 20812867
    29. Identification of 951 genes altered in transcription significantly and specifically by the expression of the functional TRPM7 channel. PMID: 21471721
    30. Ca and Mg homeostasis ensured by TRPM7 expression is important for the osteoblastic differentiation of MC3T3 cells. PMID: 21207015
    31. Depletion of TRPM7 interferes with the signalling pathways required for lamellipodial formation, cell polarization and directional cell migration. PMID: 21208190
    32. Data report that a synthetic serine protease inhibitor, nafamostat mesylate (NM), and several of its analogues, block TRPM7 currents in inverse relationship to the concentration of extracellular divalent cations. PMID: 21122141
    33. TRPM7 is required for MSC survival and perhaps involved in the differentiation process. PMID: 19929312
    34. Data show that activation of TRPM7 channels augmented Zn(2+)-induced injury of cultured mouse cortical neurons. PMID: 20048154
    35. Results establish that TRPM7 is the major Ca(2+)-permeable channel in human atrial fibroblasts and likely plays an essential role in TGF-beta1-elicited fibrogenesis in human atrial fibrillation. PMID: 20075334
    36. excessive TRPM7 channel activity causes oxidative and nitrosative stresses, producing cell rounding mediated by p38 MAPK/JNK-dependent activation of m-calpain PMID: 20070945
    37. The Trpm7 ion channel possesses the unique ability to efficiently conduct many essential trace metal ions such as Zn2+, Fe2+, Cu2+, Mn2+, and Co2+ into cells, along with several toxic metal ions such as Cd2+, Ba2+, and Sr2+ as well. PMID: 12508053
    38. TRPM6 specifically interacted with its closest homolog, the Mg(2+)-permeable cation channel TRPM7, resulting in the assembly of functional TRPM6/TRPM7 complexes at the cell surface. PMID: 14976260
    39. phosphorylation of annexin 1 at Ser5 by TRPM7 kinase may modulate function of annexin 1 PMID: 15485879
    40. TRPM7 is a functionally important regulator of Mg2+ homeostasis and growth in vascular smooth muscle cells. PMID: 15591230
    41. current activity and magnesium regulation of transient receptor potential cation channel, subfamily M, member 7(TRPM7)is not affected by kinase activity or autophosphorylation but kinase domain may play role in channel assembly or subcellular localization PMID: 15781465
    42. TRPM7 is required for intestinal pacemaking PMID: 16285951
    43. TRPM7 contributes to the mechanism by which hippocampal neurons "detect" reductions in extracellular divalents and provide a means by which TRPM7 contributes to neuronal death during transient brain ischemia. PMID: 17913893
    44. altered Mg(2+) metabolism in hyperaldosteronism may relate to TRPM7 downregulation PMID: 18268139
    45. deletion of Trpm7 showed it is essential for embryo development; tissue-specific deletion of Trpm7 in T cell lineage disrupted thymopoiesis, which led to developmental block of thymocytes at the double-negative stage & depletion of thymic medullary cells PMID: 18974357
    46. Alterations in magnesium status and/or TRPM7 signaling may contribute to vascular injury in conditions associated with hypomagnesemia. PMID: 19103997
    47. covalent modifications or binding interactions that alter the conformation of the N-terminal exchanged segment may provide a means to regulate TRPM7 kinase activity. PMID: 19228120

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  • 亞細(xì)胞定位：
    Membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Protein kinase superfamily, Alpha-type protein kinase family, ALPK subfamily; Transient receptor (TC 1.A.4) family, LTrpC subfamily, TRPM7 sub-subfamily
  • 組織特異性：
    Found to be expressed in brain and skeletal muscle, with stronger signals in kidney, heart, liver and spleen.
  • 數(shù)據(jù)庫鏈接：