Your Good Partner in Biology Research

  1. 首頁(yè)
  2. 蛋白
  3. 重組蛋白
  4. Recombinant Mouse V (D)J recombination-activating protein 1, partial

Recombinant Mouse V (D)J recombination-activating protein 1, partial

  • 中文名稱：
    小鼠Rag1重組蛋白
  • 貨號(hào)：
    CSB-YP322249MO
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Rag1重組蛋白
  • 貨號(hào)：
    CSB-EP322249MO
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Rag1重組蛋白
  • 貨號(hào)：
    CSB-EP322249MO-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Rag1重組蛋白
  • 貨號(hào)：
    CSB-BP322249MO
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Rag1重組蛋白
  • 貨號(hào)：
    CSB-MP322249MO
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    Rag1; V(D)J recombination-activating protein 1; RAG-1) [Includes: Endonuclease RAG1; EC 3.1.-.-); E3 ubiquitin-protein ligase RAG1; EC 2.3.2.27; RING-type E3 ubiquitin transferase RAG1)]
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長(zhǎng)度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In addition to its endonuclease activity, RAG1 also acts as an E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3. Histone H3 monoubiquitination is required for the joining step of V(D)J recombination. Mediates polyubiquitination of KPNA1.
  • 基因功能參考文獻(xiàn)：
    1. this study determined that the depletion of uterine innate lymphoid cells in Rag1-/- mice resulted in impaired uterine spiral artery remodeling and reduced fetal viability PMID: 28915450
    2. Describe spontaneous S. xylosus infection in a genetically modified murine model. S. xylosus infection in Rag1-/-Tpl2-/- mice correlated with disseminated bacteria and elevated numbers of circulating monocytes. PMID: 28830581
    3. This study demonstrated that the lymphocyte-deficient Rag1 animals demonstrated a significantly lower rate of aneurysm formation and rupture. PMID: 27416931
    4. a novel RAG1/2-mediated insertion pathway distinct from DNA transposition and trans-V(D)J recombination that destabilizes the genome and shares features with reported oncogenic DNA insertions. PMID: 28179379
    5. loss of the BH3-only protein BIM accelerated lymphoma development in p53-deficient mice. This process was negated by concomitant loss of RAG1/2-mediated antigen receptor gene rearrangement. PMID: 27621418
    6. Nonamer binding domain (NBD) of RAG1 plays a central role in the recognition of recombination signal sequence. PMID: 26742581
    7. Overall, these findings suggest that the V(D)J recombination-activating gene 1, RAG1, may play a role in long-term memory consolidation. PMID: 26843989
    8. propose that pre-B cells toggle between pre-BCR signals and a RAG1 DNA double-strand break-dependent checkpoint to maintain genome stability while iteratively assembling Igl chain genes PMID: 26834154
    9. Study reports that RAG1 associates with chromatin at thousands of active promoters and enhancers in the genome of developing lymphocytes. The mouse and human genomes appear to have responded by reducing the abundance of "cryptic" recombination signals near RAG1 binding sites. PMID: 26234156
    10. The non-core region of RAG1 facilitates chromosomal V(D)J recombination in a ubiquitylation-dependent pathway. PMID: 25572281
    11. Based on our results, we propose that interaction of RAG2 with RAG1 induces cooperative interactions of multiple binding sites, induced through conformational changes at the RAG1 interdomain boundary PMID: 25676158
    12. 12recombination signal sequences-23recombination signal sequences cooperation (the "12/23 rule") is important not only for regulating RAG-mediated DNA cleavage but also for the efficiency of RAG1 and RAG2 recruitment to chromatin. PMID: 26303526
    13. ubiquitination of RAG1, which can be promoted by RAG1's own ubiquitin ligase activity, plays a significant role in governing the level of V(D)J recombination activity PMID: 26124138
    14. The complex RAG1/2 binds one copy each of 12 recombination signal sequence and 23 recombination signal sequence DNA. PMID: 25903130
    15. SATB1 binds to the ASE and Rag promoters, facilitating inclusion of Rag2 in the chromatin hub and the loading of RNA polymerase II to both the Rag1 and Rag2 promoters. PMID: 25847946
    16. Study provides evidence that RAG expression (RAG1, RAG2)in uncommitted hematopoietic progenitors and NK cell precursors marks functionally distinct subsets of NK cells in the periphery, demonstrating both a novel role for RAG in the functional specialization of the NK cell lineage and a physiological role for RAG proteins outside of V(D)J recombination. PMID: 25259923
    17. Rag1 plays a role in optic neuropathy as a proapoptotic candidate in p50-deficient mice. PMID: 25312244
    18. crystal structure of the mouse RAG1-RAG2 complex at 3.2 A resolution PMID: 25707801
    19. Integrated genetic approaches identify the molecular mechanisms of Sox4 in early B-cell development: intricate roles for RAG1/2 and CK1epsilon. PMID: 24786772
    20. Fas signals act as a silencing mechanism for CD40-induced RAGs and prevent CD40 translocation to the nucleus. PMID: 24037181
    21. noncore RAG1 regions establish a diverse TCR repertoire by overcoming Vbeta RSS inefficiency to promote Vbeta recombination and alphabeta T cell development, and by modulating TCRbeta and TCRalpha gene segment utilization. PMID: 24415779
    22. Rag1-deficient NOD thymocytes have T cell defects that can collapse regulatory boundaries at two early T cell checkpoints, which may predispose them to both leukemia and autoimmunity. PMID: 23440410
    23. HMGB1 binds more tightly to a RAG1-DNA complex over RAG1 or DNA alone. PMID: 23325855
    24. histological analysis of brain sections did not reveal a difference in reactive astrogliosis, tissue destruction, and neuronal cell death in Rag1(-/-) compared to wild-type mice PMID: 22335783
    25. The defects of scid HSCs are independent of their inability to perform lymphopoiesis because a similar defect in hematopoietic niche occupancy was not observed with Rag1(-/-) recipients. PMID: 22147896
    26. multi-subunit cullin RING E3 ligase complex VprBP/DDB1/Cul4A/Roc1 associates with full-length RAG1 through VprBP PMID: 22157821
    27. In the present study, the authors report that heteroduplex DNA containing a bubble region can be cleaved efficiently when present along with a recombination signal sequence (RSS) in cis or trans configuration. PMID: 22119487
    28. Kinetic analysis using the first highly active full-length purified Rag1/Rag2 complexes has now allowed us to define the important catalytic features of this complex. PMID: 22064481
    29. central non-core domain of RAG1 functions in nuclear localization, zinc coordination, and interactions with nucleic acid PMID: 21599978
    30. combined loss of p19Arf and Rag1 results in B-cell precursor leukemia in mice PMID: 21622646
    31. phosphorylation of RAG1 or RAG2 by ATM or DNA-PKcs at SQ/TQ consensus sites is dispensable for the joining step of V(D)J recombination. PMID: 21742970
    32. data trace the origin of the impairment in social recognition memory in RAG1-deficient mice to the RAG1 gene locus and implicate RAG1 in memory formation PMID: 21354115
    33. sequences of the endogenous TCR transcripts suggested that gene recombination could occur, albeit quite inefficiently, in the RAG-deficient mice PMID: 20454452
    34. Data show that negative regulatory function of the noncore regions of RAG1/2 limits the RAG endonuclease activity in the absence of an activating methylated histone tail bound to the complex. PMID: 21149691
    35. Data show that promoters and transcription direct RAG1 binding locally, and that RAG1 binding can be targeted in the absence of RAG2. PMID: 21115692
    36. Abcc6 and Rag1 have roles in ectopic mineralization in a murine model of pseudoxanthoma elasticum PMID: 20185580
    37. Siglec-G-deficient B1a lymphocytes survive longer in vitro and become the dominant population when injected in competition with wild-type B1a cells into Rag1-deficient mice. PMID: 20729333
    38. The stochastic generation of an autoreactive B cell repertoire is an important aspect of immunodeficiencies associated with hypomorphic RAG mutations. PMID: 20547827
    39. Liver natural killer cells may represent an independent pool of cells from those developing out of the bone marrow, and RAG-1 itself may have a significant role in NK cell development. PMID: 19949422
    40. In V(D)J recombination, while RAG1 binding was detected only at regions containing recombination signal sequences, RAG2 binds at thousands of sites in the genome containing histone 3 trimethylated at lysine 4. PMID: 20398922
    41. Essential events in B lymphopoiesis are not tightly synchronized; some progenitors with increased probability of becoming lymphocytes express RAG-1 while still part of the lineage marker-negative Sca-1+c-Kit(high) fraction. PMID: 20007571
    42. These results further underscore the similarities between RAG1/2 and integrase and suggest that certain integrase inhibitors may have the potential to interfere with aspects of B and T cell development PMID: 11756686
    43. increased accumulation of hybrid V(D)J joins in cells expressing truncated versus full-length RAGs PMID: 11779512
    44. The peripheral t-cells of Rag1KO/sf/Y OVA mice do not tolerize normally, even when twice the dose that causes tolerance in control mice is administered. PMID: 11900414
    45. RAG-mediated V(D)J recombination is not essential for tumorigenesis in Atm-deficient mice. PMID: 11940674
    46. Mutational analysis of RAG-1 revealed several amino acids outside of the canonical nonamer-binding domain that are critical for DNA binding, step arrest mutants with defects in nicking or hairpin formation, and four RAG-1 mutants defective for joining. PMID: 11971977
    47. Signal ends are more abundant than coding ends. Signal joints form only after RAG expression is downregulated. Signal joints are not inert; they are cleaved in vivo and in vitro by a nick-nick mechanism to form a substrate for RAG-mediated transposition. PMID: 11983177
    48. bcl-xL and RAG genes are induced and the response to IL-2 enhanced in EmuEBNA-1 transgenic mouse lymphocytes PMID: 12140768
    49. A RAG1/2 multimer that can cleave both RSS assembles on an isolated RSS. Complementary RSS enters this complex as naked DNA. When RAG1/2 binds 12 & 23 RSSs separately prior to their mixing, synaptic complex assembly & cleavage activity are reduced. PMID: 12145216
    50. RAG1 mutants proficient in DNA cleavage but defective in interaction with coding ends after cleavage & in capturing target DNA for transposition have defects in hybrid joint formation, hairpin coding end opening, transposition, & in V(D)J recombination. PMID: 12154124

    顯示更多

    收起更多

  • 亞細(xì)胞定位：
    Nucleus.
  • 蛋白家族：
    RAG1 family
  • 組織特異性：
    Maturing lymphoid cells and central nervous system.
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: mmu:19373

    STRING: 10090.ENSMUSP00000077584

    UniGene: Mm.828