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  4. Recombinant Mouse V (D)J recombination-activating protein 2

Recombinant Mouse V (D)J recombination-activating protein 2

  • 中文名稱：
    小鼠Rag2重組蛋白
  • 貨號：
    CSB-YP324259MO
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Rag2重組蛋白
  • 貨號：
    CSB-EP324259MO
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Rag2重組蛋白
  • 貨號：
    CSB-EP324259MO-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Rag2重組蛋白
  • 貨號：
    CSB-BP324259MO
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Rag2重組蛋白
  • 貨號：
    CSB-MP324259MO
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    Rag2; Rag-2; V(D)J recombination-activating protein 2; RAG-2
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    full length protein
  • 表達區(qū)域：
    1-527
  • 氨基酸序列
    MSLQMVTVGH NIALIQPGFS LMNFDGQVFF FGQKGWPKRS CPTGVFHFDI KQNHLKLKPA IFSKDSCYLP PLRYPATCSY KGSIDSDKHQ YIIHGGKTPN NELSDKIYIM SVACKNNKKV TFRCTEKDLV GDVPEPRYGH SIDVVYSRGK SMGVLFGGRS YMPSTQRTTE KWNSVADCLP HVFLIDFEFG CATSYILPEL QDGLSFHVSI ARNDTVYILG GHSLASNIRP ANLYRIRVDL PLGTPAVNCT VLPGGISVSS AILTQTNNDE FVIVGGYQLE NQKRMVCSLV SLGDNTIEIS EMETPDWTSD IKHSKIWFGS NMGNGTIFLG IPGDNKQAMS EAFYFYTLRC SEEDLSEDQK IVSNSQTSTE DPGDSTPFED SEEFCFSAEA TSFDGDDEFD TYNEDDEDDE SVTGYWITCC PTCDVDINTW VPFYSTELNK PAMIYCSHGD GHWVHAQCMD LEERTLIHLS EGSNKYYCNE HVQIARALQT PKRNPPLQKP PMKSLHKKGS GKVLTPAKKS FLRRLFD
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Core component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. DNA cleavage by the RAG complex occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3.
  • 基因功能參考文獻：
    1. the zinc finger motif within the noncore region of RAG2 is indispensable for maintaining the stability of the RAG2 protein. PMID: 26692406
    2. evolution of RAG1/RAG2 began with a Transib transposon whose intrinsic recombination activity was enhanced by capture of an ancestral RAG2, allowing for the development of adaptive immunity. PMID: 27056670
    3. Rag2(R229Q) knock-in mice developed an inflammatory bowel disease affecting both the small bowel and colon. PMID: 26926994
    4. Rag2 truncation substantially increased the frequency of off-target V(D)J recombination. The data suggest that interactions between Rag2 and a specific chromatin modification, H3K4me3, support V(D)J recombination fidelity. PMID: 26365182
    5. These results reveal an unanticipated functional interplay between the RAG complex and XLF in repairing RAG-induced DNA breaks and maintaining genome integrity during antigen receptor gene assembly. PMID: 26833222
    6. This study demonstrated that The behavioral impairments in Rag2(-/-) mice were paralleled by an elevation in plasma corticosterone after behavioral tests. PMID: 25257108
    7. V(D)J recombination is stimulated by binding of H3K4me3 to RAG-2 PMID: 25543141
    8. Rag2-/-Il1rn-/- mice develop spontaneous severe colitis with high mortality. PMID: 24770649
    9. Both the RAG2 C-terminus and ATM prevent bi-locus RAG-mediated cleavage through modulation of three-dimensional conformation (higher-order loops) and nuclear organization of the two loci. PMID: 23900513
    10. Humanized Rag2 -/- gammac -/- mice produce the major target cells of HIV-1 and these cells can be detected in primary and secondary lymphoid tissues, as well as in the vaginal and rectal mucosa and brain tissues PMID: 23824904
    11. Hypomorphic mutation in the RAG2 gene affects dendritic cell distribution and migration. PMID: 24052573
    12. Data show that recombination activating gene-2 (Rag2), is required for toll-like receptor 9 (TLR9)-mediated induction of interferon-alpha (IFNalpha) in bone marrow-derived dendritic cells (pDCs). PMID: 23110142
    13. Bidirectional activity of the NWC promoter is responsible for RAG-2 transcription in non-lymphoid cells PMID: 22984564
    14. USF-1 but not USF-2 is strongly enriched at Dbeta2 in chromatin from either Rag2-/- (deficient) thymocytes or Rag2-/- thymocytes that express a rearranged Tcrb transgene. PMID: 22287717
    15. Using recombination activation gene-2-deficient (RAG-2(-/-)) blastocysts and embryonic stem cells with homozygous NFI-A gene deletion, we show an essential role for NFI-A in T-cell activation. PMID: 21602176
    16. hSIRPa-transgenic Rag2(-/-)gamma(c)(-/-) mice represent a unique mouse strain supporting high levels of human cell engraftment. PMID: 21788509
    17. results reveal a novel genome guardian role for RAG2 and suggest that similar 'end release/end persistence' mechanisms underlie genomic instability and lymphomagenesis in Rag2(c/c) p53(-/-) and Atm(-/-) mice PMID: 21368836
    18. Careful analysis of the Rag2 intergenic region uncovered a second evolutionarily conserved Nwc promoter region from which a previously unknown Nwc transcript can be generated in nonlymphocytes of Nwctmpro1 and normal mice. PMID: 21243488
    19. Rag2-deficient mice given Ccr6(null)CD4(positive)CD45RB(high-expressing) T cells had more severe colitis with increased interferon (IFN) gamma-producing T cells, compared with the mice given wild-type cells. PMID: 20720211
    20. Rag2 knockout mice were not able to clear up influenza A virus infection. PMID: 20667098
    21. Impaired B cell receptor (BCR) editing and a serum B cell activating factor (BAFF) abundance might contribute toward the development of a pathogenic B cell repertoire in hypomorphic Rag2(R229Q) knock-in mice. PMID: 20547828
    22. These findings unambiguously demonstrate postthymic, RAG-dependent TCR rearrangement and define TCR revision as a tolerance pathway that targets mature peripheral CD4(+) T cells. PMID: 20435935
    23. The tissue- and stage-specific expression of RAG-2 is regulated by alteration of the chromatin structure as well as cis-regulatory enhancer elements during lymphocyte ontogeny. PMID: 12097391
    24. generates mature T-and B-cells PMID: 12100900
    25. Rag2 interacts with both wt and mutant RAG1 proteins equally well and forms RAG1-RAG2-RSS complexes. PMID: 12154124
    26. unresolved RAG-2 mediated breaks during V(D)J recombination trigger a nonreciprocal replication pathway (i.e., break-induced-replication), leading to the juxtaposition of IgH and c-myc loci. PMID: 12186839
    27. molecular mechanism of the rapid cytoplasmic sequestration/degradation of the RAG2 protein PMID: 12205088
    28. effect of Rag2 gene transfer to rag2-/- mice on t-cell and b-cell reconstitution and function PMID: 12393742
    29. RAG-dependent peripheral TCR diversification occurs in T8-cells. When antigen-specificT8 cells from NOD mice were stimulated by dendritic cells, daughter cells reexpressed RAGs, stopped making antigen-specific TCRs, & made a vast array of other TCRs.. PMID: 12432095
    30. the RAG2 C terminus has potential roles in mediating rearrangement of endogenous antigen-receptor loci PMID: 12531919
    31. intrinsic RAG-2 NLS functions in the nuclear uptake of RAG-2 following its reexpression in cycling cells PMID: 12861017
    32. Deficiency is required to induce pathologic immune responses against the islet self-antigens. PMID: 15210748
    33. Injection of CD34+ cells into newborn Rag2(-/-) mice conferred resistance to gamma-irradiation. PMID: 15319293
    34. the effect of HMGA1 on RAG2 expression is direct; identification of the responsible region in the RAG2 promoter and demonstration of binding to the promoter in vivo using chromatin immunoprecipitation PMID: 15713121
    35. A transgenic rag2-EGFP-mMyc zebrafish line that develops GFP-labeled T cell acute lymphoblastic leukemia. PMID: 15827121
    36. PHD finger, a zinc finger, is a novel and functionally important domain of RAG2. PMID: 15964836
    37. Purified RAG2 protein from thymocytes of young mice associates with endogenous histone in a manner that is dependent on the RAG2 carboxyl terminus PMID: 16111638
    38. should be valuable to study virus-induced pathology and to rapidly evaluate new approaches aiming to prevent or treat HIV infection PMID: 17038503
    39. A knockin murine model carrying the Rag2 R229Q mutation previously described in several patients with Omenn syndrome (OS)supports the notion that impaired immune tolerance and defective immune regulation are involved in the pathophysiology of OS. PMID: 17476358
    40. RAG2(-/-) mice demonstrated fatigue with shorter inverted hang-times (HT) and voluntary wheel-running (VWR) distance and total run times; they increased body weight more slowly but had proportionally normal forelimb grip strength (FGS) and VWR speed. PMID: 17554799
    41. The crystal structures of the RAG2 plant homeodomain alone & complexed with 5 modified H3 peptides show that this one domain simultaneously recognizes 2 distinct histone modifications, permitting complex read-out of combinatorial histone modifications. PMID: 18025461
    42. The high-resolution crystal structure of the mouse RAG2 PHD finger bound to H3K4me3 reveals the molecular basis of H3K4me3-recognition by RAG2. PMID: 18033247
    43. Endoplasmic reticulum stress plays an important role in cell death and cardiac dysfunction in beta(1)-extracellular loop (EC)(II) IgG cardiomyopathy, and the effects of beta(1)-EC(II) IgG are mediated via the beta(1)-adrenergic receptor. PMID: 18155231
    44. These results indicate that accessibility of NWC promoter and RAG promoters to trans-acting factors is regulated by different epigenetic mechanisms. PMID: 18166226
    45. The effect of the enterohepatic bacterial pathogen Helicobacter hepaticus on liver and intestine tumorigenesis in BALB-RagMin mice is reported. PMID: 18411292
    46. In mice lacking the non-core domain of RAG2 the ordered rearrangement is disturbed and direct V to D rearrangements are 10- to 1000-times increased in tri-partite immune gene loci. PMID: 18776220
    47. humanized mice showed low levels of human engraftment in the intestinal tract and seem to be resistant to rectal transmission of HIV PMID: 18842716
    48. These results reveal different strategies of Epstein-Barr virus infection in B cells that possibly result from variations in the host immune response. PMID: 18845836
    49. In scid mice, the frequency of Rag2-mediated rearrangements was relatively low compared with that in wild-type mice, suggesting that the Rag2-independent lymphoma pathway prevails in the development of thymic lymphomas in scid mice. PMID: 19000702
    50. These results confirm essential roles for inflammation, increased TNF-alpha expression, and elevated nitric oxide production in colon carcinogenesis. PMID: 19164562

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  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    RAG2 family
  • 組織特異性：
    Maturing lymphoid cells.
  • 數(shù)據庫鏈接：