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Recombinant Mouse Wiskott-Aldrich syndrome protein homolog (Was)

  • 中文名稱：
    小鼠Was重組蛋白
  • 貨號：
    CSB-YP025967MO
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    小鼠Was重組蛋白
  • 貨號：
    CSB-EP025967MO
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    小鼠Was重組蛋白
  • 貨號：
    CSB-EP025967MO-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    小鼠Was重組蛋白
  • 貨號：
    CSB-BP025967MO
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    小鼠Was重組蛋白
  • 貨號：
    CSB-MP025967MO
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    Was; Wasp; Wiskott-Aldrich syndrome protein homolog; WASp
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Full length protein
  • 表達區(qū)域：
    1-520
  • 氨基酸序列
    MNSGPGPVGG RPGGRGGPAV QQNIPSNLLQ DHENQRLFEL LGRKCWTLAT TVVQLYLALP PGAEHWTMEH CGAVCFVKDN PQKSYFIRLY GLQAGRLLWE QELYSQLVYL TPTPFFHTFA GDDCQVGLNF ADESEAQAFR ALVQEKIQKR NQRQSGERRQ LPPPPAPINE ERRGGLPPVP PHPGGDHGGP SGGPLSLGLV TVDIQNPDIT SSRYRGLPAP GPGPTDKKRS GKKKISKADI GAPSGFKHVS HVGWDPQNGF DVNNLDPDLR SLFSRAGISE AQLTDAETSK LIYDFIEDQG GLEAVRQEMR RQEPLPPPPP PCRGGGGGGG GGGGGGGGGG GQPLRPPVVG SNKGRSGPLP PVPMGGAPPP PTPRGPPPPG RGGPPPPPPP ATGRSGPPPP PLPGAGGPPA PPPPPPPPPP PPCPGSGPAP PPLPPTPVSG GSPAPGGGRG ALLDQIRQGI QLNKTPGALE NSVQQPPAQQ SEGLVGALMH VMQKRSRVIH SSDEGEDQTG EDEEDDEWDD
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Effector protein for Rho-type GTPases that regulates actin filament reorganization via its interaction with the Arp2/3 complex. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function. Mediates actin filament reorganization and the formation of actin pedestals upon infection by pathogenic bacteria. In addition to its role in the cytoplasmic cytoskeleton, also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. Promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).
  • 基因功能參考文獻：
    1. In ex vivo models of enteropathogenic Escherichia coli and Shigella flexneri infection, WASp deficiency causes defective bacterial clearance, excessive inflammasome activation and host cell death that are associated with dysregulated septin cage-like formation, impaired autophagic p62/LC3 recruitment and defective formation of canonical autophagosomes. PMID: 29146903
    2. These data reveals an intricate balance between activation of WASp and Rac2 signalling pathways in dendritic cells. PMID: 27425374
    3. Was(-/-) mice provide a model of food allergy with the advantage of mimicking polysensitization and low food-antigen IgE titers as observed in humans with clinical food allergy. PMID: 28600891
    4. WASp knockout mice controlled growth of A20 lymphoma cells that naturally produced IL-2. PMID: 27477778
    5. We conclude that CRN7 spatiotemporally influences F-actin organization and Golgi integrity in a Cdc42- and N-WASP-dependent manner. PMID: 27143109
    6. DOCK8 and WASp are in the same signaling pathway that links T-cell receptors (TCRs) to the actin cytoskeleton in TCR-driven actin assembly. PMID: 27599296
    7. the dendritic cell actin cytoskeleton organized into recognizable synaptic structures, formed Wiskott-Aldrich syndrome protein-dependent podosomes within this area. PMID: 26590149
    8. N-WASP expression in B lymphocytes is required for the development of autoimmunity of WAS (Wiskott-Aldrich syndrome) and may represent a novel therapeutic target in WAS. PMID: 26468226
    9. Platelet actin nodule formation is dependent on WASp and the ARP2/3 complex. WASp(-/-) mouse blood displays impaired platelet aggregate formation at arteriolar shear rates. PMID: 26028144
    10. Findings suggest that Wiskott-Aldrich syndrome protein (WASp) plays a crucial role in B10 cell development and that WASp-deficient B10 cells may contribute to autoimmunity in Wiskott-Aldrich syndrome. PMID: 25728049
    11. These results strongly suggest that the association between the WASP N-terminal domain and Btk plays an important role in the TLR2-signaling pathway in macrophages. PMID: 25213142
    12. These findings support a contributory role for defective Breg cells in the development of WAS-related autoimmunity PMID: 24945741
    13. Development of central nervous system autoimmunity is impaired in the absence of Wiskott-Aldrich syndrome protein. PMID: 24466296
    14. In the absence of Wiskott-Aldrich syndrome protein, effective hematopoietic stem cell mobilization is achieved. PMID: 23445877
    15. Data indicate that Wiskott-Aldrich syndrome protein (WASP) deficiency affects resistance to tumor and causes impairment in the antitumor capacity of natural killer and dendritic cells. PMID: 24338698
    16. tyrosine phosphorylation of WASP by Hck is required for proper macrophage functions PMID: 24482227
    17. Colitis and colon cancer in WASP-deficient mice require helicobacter species. PMID: 23820270
    18. T cell receptor signaling causes WASP to be degraded by calpain and by Cbl-family members through ubiquitination and destruction by the proteasome, limiting TCR-driven assembly of F-actin. PMID: 23684068
    19. Aldolase inhibits WASP/Arp2/3-dependent actin polymerization in vitro PMID: 23495010
    20. Increased uptake by splenic red pulp macrophages contributes to rapid platelet turnover in WASP(-) mice. PMID: 23727585
    21. By acute silencing of WASp and actin inhibitors, we show that WASp-mediated actin polymerization controls intracellular trafficking and compartmentalization of TLR9 ligands in dendritic cells restraining exaggerated activation of the TLR9-IFN-alpha pathway. PMID: 23337808
    22. Cdc42 regulates neutrophil migration via crosstalk between WASp, CD11b, and microtubules in the process of chemotaxis. PMID: 22932798
    23. WASP-deficient aberrant innate immune cells can render wild-type T cells severely colitogenic. PMID: 22710191
    24. These observations strongly suggest that the phosphorylation of WASP by Btk plays a pivotal role in transducing the LPS signaling pathway in macrophages. PMID: 22634306
    25. Ubiquitous high-level gene expression in hematopoietic lineages provides effective lentiviral gene therapy of murine Wiskott-Aldrich syndrome. PMID: 22431569
    26. Data show that the protrusions from WASp-deficient cells reduced persistence compared to their respective control shRNA and wild-type cells. PMID: 22279563
    27. These observations strongly suggest that the interaction between WASP N-terminal domain and Btk plays important roles in the lipopolysaccharide signaling cascade in innate immunity. PMID: 22253930
    28. suggesting that WASp-dependent B cell-intrinsic mechanisms critically contribute to WAS-associated autoimmunity PMID: 22302739
    29. WASp-deficient mice develop proliferative glomerulonephritis reminiscent of human IgA nephropathy (IgAN). Serum IgA and IgA production by splenic B cells was increased in WASp-deficient mice compared to wild-type mice. PMID: 22079330
    30. study reports that a heterotrimeric protein complex exists between PLD2, Grb2, and WASp in vivo, which is integral to phagocytosis PMID: 21930784
    31. the WASP N-terminal domain plays a pivotal role in the TCR signaling cascade by binding to Fyn. PMID: 21705469
    32. There are significant defects in both organization and function of the immunologic synapse when DCs alone lack WASp. This results in abrogated TCR-mediated signaling and priming. PMID: 21690559
    33. Data demonstrate that mutation of the WAS gene results in B cells that are hyperresponsive to B cell receptor and Toll-like receptor (TLR) signals in vitro, thereby promoting a B cell-intrinsic break in tolerance. PMID: 21875954
    34. Preactivated Was Knock-out nTreg cells failed to effectively suppress B-cell proliferation and that such a defect was associated with reduced killing of B cells and significantly decreased degranulation of granzyme B. PMID: 21515824
    35. Studies show that HS1 functions in concert with WASp to fine-tune DC cytoarchitecture and direct cell migration. PMID: 21398607
    36. In macrophages, many of the defects associated with the loss of WASP, such as podosome-dependent matrix degradation and chemotaxis were corrected when N-WASP was expressed at equimolar level to that of the wild-type WASP. PMID: 20599953
    37. WASp-deficient mice make autoantibodies & develop proliferative glomerulonephritis with immune complex deposition as they age. Deficiency affects apoptosis & TCR-induced secretion of FasL & other secretory granule components by CD4(+) T cells. PMID: 20457871
    38. These data indicate that there are unique requirements for the presence and activation status of WASP in B and T cells and that WASP-activating mutations interfere with lymphocyte cell survival and genomic stability. PMID: 20513746
    39. WASp plays a selective, posttranscriptional role in Th2 effector function. PMID: 20032499
    40. Deficiency leads to baldness, and acts as a positive regulator of beta-catenin-dependent transcription, modulating differentiation of hair follicle progenitor cells. PMID: 20071778
    41. Cdc42 binding and activation of WASp are required for podosome formation and chemotaxis. PMID: 19808890
    42. novel role for WASP in the regulation of TCR-induced integrin clustering and cell adhesion PMID: 11911819
    43. Platelets activate Arp2/3 complex, assemble actin, and change shape in the absence of WASp, indicating a more specialized role for WASp in these cells. PMID: 12200375
    44. Itk is a key element of the pathway leading to localized actin polymerization at the immune synapse through localized activation of Cdc42 and WASP. PMID: 13678593
    45. results provide evidence that WASp is a critical component of podosomes in osteoclasts and indicate a nonredundant role for WASp in the dynamic organization of actin structures during physiologic bone resorption PMID: 14726392
    46. WASp is either not involved in or is redundant in the rapid dynamics of lymphocyte microvilli. PMID: 15130947
    47. Although WASP may facilitate immune synapse formation at low peptide concentrations, it is not required for this process. The role of WASP in regulating IL-2 production is independent of its role in immune synapse formation. PMID: 15265894
    48. 5 mutational hotspots in the WASP gene have been able to establish a strong association between genotype and phenotype in Wyskott-Aldrich syndrome. PMID: 15284122
    49. WASp is crucial for optimal B-cell responses and plays a pivotal role in the primary humoral immune response. as shown in knockout mice. PMID: 15383456
    50. WASp is a key molecule for the regulated migration and trafficking of dendritic cells in vivo; defects in this process may promote abnormalities of immune response. PMID: 15494425

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  • 亞細胞定位：
    Cytoplasm, cytoskeleton. Nucleus.
  • 數(shù)據(jù)庫鏈接：