Your Good Partner in Biology Research

  1. 首頁
  2. 蛋白
  3. 重組蛋白
  4. Recombinant Plasmodium falciparum L-lactate dehydrogenase, partial

Recombinant Plasmodium falciparum L-lactate dehydrogenase, partial

  • 中文名稱：
    Recombinant Plasmodium falciparum L-lactate dehydrogenase,partial,Yeast
  • 貨號：
    CSB-YP318413PLO
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Plasmodium falciparum L-lactate dehydrogenase,partial,Yeast
  • 貨號：
    CSB-EP318413PLO-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Plasmodium falciparum L-lactate dehydrogenase,partial,Yeast
  • 貨號：
    CSB-BP318413PLO
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Plasmodium falciparum L-lactate dehydrogenase,partial,Yeast
  • 貨號：
    CSB-MP318413PLO
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    N/A
  • Uniprot No.：
  • 別名：
    L-lactate dehydrogenase; EC 1.1.1.27; LDH-P; Fragment
  • 種屬：
    Plasmodium falciparum
  • 蛋白長度：
    Partial
  • 表達區(qū)域：
    1-42
  • 氨基酸序列
    LLVYDNLLLN DKNEMNKDSS YDKKTNALDN YKNDSTIDME KK
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

問答及客戶評論

■ 常見問題解答
Q:

Please can you provide informations for all available sizes of all available expression hosts?
Have you received any customer feedback about the activity of this protein?
Could you clarify the molecular weight, any fusion proteins, and part of this recombinant that is PfLDH?

A:
Very nice to receive your inquiry.
1. General Information
Recombinant Plasmodium falciparum L-lactate dehydrogenase
CSB-YP318413PLO >> Yeast
CSB-EP318413PLO >> E.coli
CSB-BP318413PLO >> Baculovirus
CSB-MP318413PLO >> Mammalian cell
Expression Region:1-42aa; Full Length
Tag information: EP, YP, BP, MP: Tag type will be determined during the manufacturing process.
The expected tag for each expression system is list as follows:
EP, BP, MP: N-terminal 10xHis-tagged and C-terminal Myc-tagged; YP: N-terminal 6xHis-tagged
Sequence:

LLVYDNLLLNDKNEMNKDSSYDKKTNALDNYKNDSTIDMEKK


2. Biological Activity
We haven't tested the activity of this protein and haven't received any feedback from other customer yet, so we cannot 100% guarantee that it is active.
The protein we provide is full length of mature protein and is purified under mild conditions, which should have activity in theory.
This recombinant protein is expressed according to the sequence in this link: http://www.uniprot.org/uniprot/P13774
It is different from the protein in this link: http://www.uniprot.org/uniprot/Q27743 The proteins in the above two links belong to the same type of protein.
There is literature reporting that Plasmodium falciparum L-lactate dehydrogenase expressed by E. coli system has very good biological activity. Our protein should also have good activity in theory.
3. Molecular Weight & PfLDH
Expected MW of EP: The MW of the target protein is 5 KDa. The MW of the N-terminal 10xHis-tagged and C-terminal Myc-tagged is 5 KDa. So the MW of the fusion protein is 10 KDa.
Expected MW of BP: The MW of the target protein is 5 KDa. The MW of the N-terminal 10xHis-tagged and C-terminal Myc-tagged is 4 KDa. So the MW of the fusion protein is 9KDa.
Expeted MW of MP: The MW of the target protein is 5 KDa. The MW of the N-terminal 10xHis-tagged and C-terminal Myc-tagged is 12 KDa (5.5KD (PTT5) + 6.5KD (pSecTag2A)).
So the MW of the fusion protein is 17 KDa.
Expected MW of YP: The MW of the target protein is 5 KDa; The MW of the N-terminal 6xHis-tagged is 2kDa. So the MW of the fusion protein is 7KDa.
As for your question "part of this recombinant that is PfLDH?", this is a short sequence of LDH that should not contain the PfLDH which the customer mentioned.
However, you could further confirm with the customer, so we could provide more information regarding this question.