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  4. Recombinant Pseudomonas aeruginosa Serralysin (aprA), partial

Recombinant Pseudomonas aeruginosa Serralysin (aprA), partial

  • 中文名稱：
  • 貨號(hào)：
    CSB-EP312664EZX1
  • 規(guī)格：
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    aprA
  • Uniprot No.：
  • 種屬：
    Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
  • 蛋白長度：
    Partial
  • 來源：
    E.coli
  • 分子量：
    50.6 kDa
  • 表達(dá)區(qū)域：
    37-479aa
  • 氨基酸序列
    PSYTVDQAAEQILREQASWQKAPGDSVLTLSYSFLTKPNDFFNTPWKYVSDIYSLGKFSAFSAQQQAQAKLSLQSWSDVTNIHFVDAGQGDQGDLTFGNFSSSVGGAAFAFLPDVPDALKGQSWYLINSSYSANVNPANGNYGRQTLTHEIGHTLGLSHPGDYNAGEGDPTYADATYAEDTRAYSVMSYWEEQNTGQDFKGAYSSAPLLDDIAAIQKLYGANLTTRTGDTVYGFNSNTERDFYSATSSSSKLVFSVWDAGGNDTLDFSGFSQNQKINLNEKALSDVGGLKGNVSIAAGVTVENAIGGSGSDLLIGNDVANVLKGGAGNDILYGGLGADQLWGGAGADTFVYGDIAESSAAAPDTLRDFVSGQDKIDLSGLDAFVNGGLVLQYVDAFAGKAGQAILSYDAASKAGSLAIDFSGDAHADFAINLIGQATQADIVV
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標(biāo)簽：
    N-terminal 6xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 基因功能參考文獻(xiàn)：
    1. AprA could contribute to pyocyanin production in the presence of several proteins or peptides PMID: 28333255
    2. Data suggest that polymerization of AprA into amyloid-like fibrils or large two-dimensional protein sheets occurs under near-physiological conditions, is rapid, involves the RTX domain, and can be controlled by regulating calcium in solution. PMID: 25232897
    3. VIrulence factors AprA (alkaline proteasae) and Cif (CF transmembrane conductance regulator inhibitory factor) perturb sodium and chloride transport and osmosis in the lung. PMID: 23836869
    4. Structural and mutational studies reveal that the conserved N-terminal residues of AprI occupy the protease active site and are essential for AprA inhibitory activity. PMID: 22154939
    5. AprA effectively degrades the TLR5 ligand monomeric flagellin and also prevents flagellin-mediated immune responses. PMID: 21901099

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Peptidase M10B family
  • 數(shù)據(jù)庫鏈接：

    KEGG: pae:PA1249

    STRING: 208964.PA1249