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Recombinant Rat Actin, cytoplasmic 1 (Actb)

  • 中文名稱：
    Recombinant Rat Actin, cytoplasmic 1(Actb),Yeast
  • 貨號：
    CSB-YP353072RA
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Rat Actin, cytoplasmic 1(Actb),Yeast
  • 貨號：
    CSB-EP353072RA
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Rat Actin, cytoplasmic 1(Actb),Yeast
  • 貨號：
    CSB-EP353072RA-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Rat Actin, cytoplasmic 1(Actb),Yeast
  • 貨號：
    CSB-BP353072RA
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Rat Actin, cytoplasmic 1(Actb),Yeast
  • 貨號：
    CSB-MP353072RA
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Actb
  • Uniprot No.：
  • 別名：
    Actb; Actin; cytoplasmic 1; Beta-actin) [Cleaved into: Actin; cytoplasmic 1; N-terminally processed]
  • 種屬：
    Rattus norvegicus (Rat)
  • 蛋白長度：
    full length protein
  • 表達區(qū)域：
    1-375
  • 氨基酸序列
    MDDDIAALVV DNGSGMCKAG FAGDDAPRAV FPSIVGRPRH QGVMVGMGQK DSYVGDEAQS KRGILTLKYP IEHGIVTNWD DMEKIWHHTF YNELRVAPEE HPVLLTEAPL NPKANREKMT QIMFETFNTP AMYVAIQAVL SLYASGRTTG IVMDSGDGVT HTVPIYEGYA LPHAILRLDL AGRDLTDYLM KILTERGYSF TTTAEREIVR DIKEKLCYVA LDFEQEMATA ASSSSLEKSY ELPDGQVITI GNERFRCPEA LFQPSFLGME SCGIHETTFN SIMKCDVDIR KDLYANTVLS GGTTMYPGIA DRMQKEITAL APSTMKIKII APPERKYSVW IGGSILASLS TFQQMWISKQ EYDESGPSIV HRKCF
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA.
  • 基因功能參考文獻：
    1. Data show that spermiation is actin- and microtubule-based cytoskeletal-dependent event, involving regulatory proteins that modify cytoskeletal organization. PMID: 29040437
    2. Data show that spermatid/phagosome transport across the epithelium during spermatogenesis requires the coordination between actin- and microtubule (MT)-based cytoskeletons. PMID: 26894662
    3. Both ZBP1 and HuD bind the zipcode element in rat beta-actin mRNA's 3' UTR. Differences between HuD and ZBP1 were observed in their binding preference to the element. PMID: 26152301
    4. Our study demonstrates that gene expression levels of reference genes commonly used in obesity-related studies, such as ACTB or RPS18, are altered by changes in acute or chronic energy status. PMID: 23736358
    5. Axonal translation of beta-actin mRNA supports axon branching and axonal translation of GAP-43 mRNA supports elongating growth. PMID: 23426659
    6. The authors hypothesize a new post-transcriptional mechanism regulating a messenger encoded by nuclear genome for a mitochondrial protein and that Hsp90 and Actin, could represent key players in cytochrome c oxidase subunit IV translation. PMID: 19703590
    7. Axonal outgrowth seen after the second of two serial axotomies is related to the ability of newly synthesized actin that is already in transit to polymerize in response to the first axotomy. PMID: 12504890
    8. The molecular mechanism and function of beta-actin mRNA localization in dendrites of cultured hippocampal neurons was studied; ZBP1 is required for the localization of beta-actin mRNA to dendrites PMID: 14614102
    9. extremely high levels of beta-actin mRNA in osteoclasts observed in developing molar tooth germ in the mandibular bone surface PMID: 14756242

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  • 亞細胞定位：
    Cytoplasm, cytoskeleton. Nucleus.
  • 蛋白家族：
    Actin family
  • 組織特異性：
    Expressed in the epididymis (at protein level).
  • 數據庫鏈接：