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Recombinant Rat C->U-editing enzyme APOBEC-1 (Apobec1)

  • 中文名稱：
    Recombinant Rat C->U-editing enzyme APOBEC-1(Apobec1),Yeast
  • 貨號：
    CSB-YP001919RA
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Rat C->U-editing enzyme APOBEC-1(Apobec1),Yeast
  • 貨號：
    CSB-EP001919RA
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Rat C->U-editing enzyme APOBEC-1(Apobec1),Yeast
  • 貨號：
    CSB-EP001919RA-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Rat C->U-editing enzyme APOBEC-1(Apobec1),Yeast
  • 貨號：
    CSB-BP001919RA
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Rat C->U-editing enzyme APOBEC-1(Apobec1),Yeast
  • 貨號：
    CSB-MP001919RA
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    Apobec1
  • Uniprot No.：
  • 別名：
    Apobec1C->U-editing enzyme APOBEC-1; EC 3.5.4.36; Apolipoprotein B mRNA-editing enzyme 1; mRNA(cytosine(6666)) deaminase 1
  • 種屬：
    Rattus norvegicus (Rat)
  • 蛋白長度：
    full length protein
  • 表達(dá)區(qū)域：
    1-229
  • 氨基酸序列
    MSSETGPVAV DPTLRRRIEP HEFEVFFDPR ELRKETCLLY EINWGGRHSI WRHTSQNTNK HVEVNFIEKF TTERYFCPNT RCSITWFLSW SPCGECSRAI TEFLSRYPHV TLFIYIARLY HHADPRNRQG LRDLISSGVT IQIMTEQESG YCWRNFVNYS PSNEAHWPRY PHLWVRLYVL ELYCIILGLP PCLNILRRKQ PQLTFFTIAL QSCHYQRLPP HILWATGLK
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Catalytic component of the apolipoprotein B mRNA editing enzyme complex which is responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in the APOB mRNA. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation.
  • 基因功能參考文獻(xiàn)：
    1. To overcome this limitation, we developed a CRISPR-Cpf1-based BE by fusing the rat cytosine deaminase APOBEC1 to a catalytically inactive version of Lachnospiraceae bacterium Cpf1. PMID: 29553573
    2. Results show that expression of APOBEC1 induces a mutator phenotype in 2 different cellular models. PMID: 25085003
    3. Apobec-1 increased COX-2 expression and COX-2 mRNA stabilization in neurogenic cells, and failed to catalyze C-to-U editing of COX-2 mRNA PMID: 23609497
    4. It was found that rat APOBEC-1 overexpression had a hypermutation pattern similar to that of APOBEC-3s on its substrate apolipoprotein B mRNA. PMID: 22326345
    5. Taken together, these results demonstrate a model in which APOBEC1 induction during encephalitis in neurons may aid in thwarting HSV-1 infection. PMID: 21775448
    6. observations allow speculation (i) that APOBEC-mediated C-to-U editing may contribute to the sequence variation of viruses that replicate entirely through RNA, and (ii) that additional cellular RNA substrates might exist for the APOBEC enzymes PMID: 15286366
    7. may have roles in epigenetic reprogramming and cell plasticity PMID: 15448152
    8. Editing by APOBEC-1 was studied in yeast using Gal4 linked to Gal80 via an apo B cassette (Gal4-C) or via a mouse Ig heavy chain gene variable region (Gal4-VH). APOBEC-1 induced C to U editing in ~15% of the Gal4-C transcripts, but did not affect Gal4-VH. PMID: 15963568

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  • 亞細(xì)胞定位：
    Cytoplasm.
  • 蛋白家族：
    Cytidine and deoxycytidylate deaminase family
  • 組織特異性：
    Expressed in the liver as well as small intestine.
  • 數(shù)據(jù)庫鏈接：