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  4. Recombinant Rat Glutamate receptor, ionotropic kainate 2 (Grik2), partial

Recombinant Rat Glutamate receptor, ionotropic kainate 2 (Grik2), partial

  • 中文名稱：
    大鼠Grik2重組蛋白
  • 貨號：
    CSB-YP009907RA
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大鼠Grik2重組蛋白
  • 貨號：
    CSB-EP009907RA
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大鼠Grik2重組蛋白
  • 貨號：
    CSB-EP009907RA-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大鼠Grik2重組蛋白
  • 貨號：
    CSB-BP009907RA
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大鼠Grik2重組蛋白
  • 貨號：
    CSB-MP009907RA
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    Grik2; Glur6Glutamate receptor ionotropic; kainate 2; GluK2; Glutamate receptor 6; GluR-6; GluR6
  • 種屬：
    Rattus norvegicus (Rat)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. Modulates cell surface expression of NETO2.; Independent of its ionotropic glutamate receptor activity, acts as a thermoreceptor conferring sensitivity to cold temperatures. Functions in dorsal root ganglion neurons.
  • 基因功能參考文獻：
    1. In the CA1 hippocampal pyramidal cell, GluK2 traffics to the neuronal surface and synapse efficiently and the auxiliary subunits Neto1 and Neto2 caused no further enhancement of these two trafficking processes. GluK2 caused a more than 10-fold increase in synaptic transmission. It is the amino-terminal domains of GluK1 and GluK2 that control their strikingly different trafficking properties. PMID: 28100490
    2. delivery of de novo kainate receptors is differentially regulated by modulation of GluK2 Q/R editing, PKC phosphorylation, and PDZ ligand interactions. PMID: 28636947
    3. structure of the kainate receptor GluK2 subtype in its desensitized state by cryo-electron microscopy at 3.8 A resolution; desensitization is characterized by the establishment of a ring-like structure in the ligand-binding domain layer of the receptor PMID: 27580033
    4. amino acids in the M3-S2 linker are critical determinants of desensitization and in part determine both the magnitude and the directionality of the Neto2 effects on KAR gating. PMID: 26282342
    5. The present study reveals an additional mechanism for the regulation of GluK2-containing kainate receptors by Src family kinases, which may be of pathological significance in ischemic stroke. PMID: 25201974
    6. These data suggest that Rab17 mediates the dendritic trafficking of Syntaxin-4 to selectively regulate dendritic surface insertion of GluK2-containing KARs in rat hippocampal neurons. PMID: 24895134
    7. These findings indicate that ligand binding to only the GluK5 subunit is both necessary and sufficient to allow trafficking of recombinant GluK2/K5 heteromers to the cell membrane, but that occupancy of the GluK2 site alone is not. PMID: 23975096
    8. characterized 3 GluK2 apical mutants with attenuated desensitization and identified conformational changes underlying their functional phenotypes; propose that the charge balance in the GluK2 LBD dimer interface maintains a degree of instability, necessary for rapid and complete desensitization PMID: 23720540
    9. Importance of a cation-binding pocket in transitions between activated and desensitized states. Tethering a positive charge to the pocket sustains activation, preventing desensitization, where mutations that disrupt cation binding eliminate gating. PMID: 23955023
    10. 14-3-3 proteins are an important regulator of GluK2a-containing KARs and may contribute to the slow decay kinetics of native KAR-EPSCs. PMID: 23861400
    11. analysis by cryoelectron tomography of full-length GluK2 kainate receptors trapped in antagonist-bound resting and agonist-bound desensitized states PMID: 23530186
    12. GluK2 and GluK5 assemble with 2:2 stoichiometry. PMID: 22509486
    13. Kainate receptor GluK2a post-translational modifications differentially regulate association with 4.1N to control activity-dependent receptor endocytosis PMID: 23400781
    14. Our results suggest a role for the dynamic control of synaptic SUMOylation in the regulation of GluK2 synaptic transmission and plasticity PMID: 22522402
    15. these results suggest that nNOS binds with GluR6 via PSD95 and then produces endogenous NO to S-nitrosylate GluR6 in cerebral ischemia-reperfusion, which provides a new approach for stroke therapy. PMID: 22445759
    16. Data show that mutagenesis of S868 phosphorylation site increased GluK2 SUMOylation. PMID: 22089239
    17. Heteromeric GluK2/K5 receptors expressed in human HEK-293T cells show markedly higher glutamate sensitivity than GluK2 homomers and do not desensitize at low glutamate concentrations. PMID: 22114280
    18. Ca2+ entry through KA receptors at the growth cone of DRG neurons may be an important regulator of neurite outgrowth. PMID: 21557511
    19. S-nitrosylation levels of Bcl-2 are down-regulated after kainate injection and the GluR6 (glutamate receptor 6) antagonist NS102 can inhibit the denitrosylation of Bcl-2 PMID: 21148565
    20. studies reveal that cross-linking either the amino terminal domain or the ligand-binding domain inhibits activation of GluK2 subtype kainate receptor. PMID: 20404149
    21. findings show kainate receptor subunit GluR6 plays an important role in the visceral pain induced by injection of formalin into the wall of the rectum PMID: 19784088
    22. Selective upregulation of kainate receptor subunit KA2 expression contributes to inflammatory hyperalgesia. PMID: 12359272
    23. The facilitatory effects of kainate on mossy fiber synaptic transmission and plasticity are mediated by GLU(K6)-containing kainate receptors. PMID: 15537878
    24. Crystal structure and binding sites of subunits. PMID: 15677325
    25. kainic acid-preferring glutamate receptors are responsive to changes in plasma glucose concentrations and may participate in the activation of the paraventricular neurones during hypoglycaemic stress. PMID: 15796762
    26. In conclusion, our results demonstrate that kainate receptor subunit GluR6 plays an important role in neuronal death induced by cerebral ischemia followed by reperfusion. PMID: 16267825
    27. Our results suggest that full and partial agonists elicit distinct conformational changes in KARs during desensitization. PMID: 16439423
    28. The GluR6 S1S2 domain has 4 nearby AAs differing between AMPA & kainate subunits. This contributes to their different desensitization kinetics. A common desensitization mechanism depends on binding domain dimer interface stability. PMID: 17005866
    29. the kainate receptor subunit GluR6 is a SUMO substrate PMID: 17486098
    30. Hypothermia decreased the increased assembly of the GluR6-PSD95-MLK3 signaling module and the activation of JNK pathway induced by I/R through KAR, giving a new insight into the ischemic therapy. PMID: 18172894
    31. Vulnerability of GluR6 to fatty acid inhibition depends on the pore loop conformation, which can be modified by specific side chain replacements. PMID: 18562501
    32. GluR6-contained KA receptors can mediate p38 MAP kinase activation through a kinase cascade. PMID: 18680160
    33. adrenalectomy results in increased expression of hippocampal GluR6 mRNA, an effect which is reversed by treatment with aldosterone... PMID: 19180187
    34. crystal structure of the amino-terminal domain from the kainate receptor GluR6 PMID: 19465914
    35. The nondesensitizing D776K mutant provides insights into kainate receptor gating and represents a potentially useful new tool for dissecting kainate receptor function. PMID: 19561126

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  • 亞細胞定位：
    Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Glutamate-gated ion channel (TC 1.A.10.1) family, GRIK2 subfamily
  • 組織特異性：
    Highest expression is found in the olfactory lobe, piriform cortex, dentate gyrus, hippocampus, granular cell layer of the cerebellum, and in caudate-putamen.
  • 數(shù)據(jù)庫鏈接：