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  4. Recombinant Rat Protein unc-13 homolog A (Unc13a), partial

Recombinant Rat Protein unc-13 homolog A (Unc13a), partial

  • 中文名稱：
    大鼠Unc13a重組蛋白
  • 貨號：
    CSB-YP723474RA
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大鼠Unc13a重組蛋白
  • 貨號：
    CSB-EP723474RA
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大鼠Unc13a重組蛋白
  • 貨號：
    CSB-EP723474RA-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大鼠Unc13a重組蛋白
  • 貨號：
    CSB-BP723474RA
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大鼠Unc13a重組蛋白
  • 貨號：
    CSB-MP723474RA
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    Unc13a; Unc13h1; Protein unc-13 homolog A; Munc13-1
  • 種屬：
    Rattus norvegicus (Rat)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. Involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-dependent refilling of readily releasable vesicle pool (RRP). Essential for synaptic vesicle maturation in most excitatory/glutamatergic but not inhibitory/GABA-mediated synapses. Facilitates neuronal dense core vesicles fusion as well as controls the location and efficiency of their synaptic release. Also involved in secretory granule priming in insulin secretion. Plays a role in dendrite formation by melanocytes.
  • 基因功能參考文獻：
    1. These results suggested that Munc13-1 has an inhibitory role in antigen-induced mast cell degranulation, which is performed in a Munc13-4-dependent manner. PMID: 29225210
    2. W22A, W22K, W22D, W22Y, and W22F substitutions were made in Munc13-1. The GFP-tagged constructs were expressed in Neuro-2a cells. Their membrane translocation in response to phorbol ester was observed in live cells by confocal microscopy. Munc13-1 translocated to the plasma membrane, the C1 domain translocated to internal membranes in response to phorbol ester. Trp-588 is important for ligand binding and translocation. PMID: 29244485
    3. The structure imposes key constraints for models of neurotransmitter release and suggests that Munc13-1 bridges the vesicle and plasma membranes from the periphery of the membrane-membrane interface. PMID: 28177287
    4. Overall, these results support a model whereby the multiple domains of Munc13 cooperate to coordinate synaptic vesicle docking, priming and fusion. PMID: 27213521
    5. Here, the authors identified two conserved residues (R151, I155) in the syntaxin-1 linker region as key sites for the Munc13-1 MUN domain interaction. This interaction is essential for SNARE complex formation in vitro and synaptic vesicle priming in neuronal cultures. PMID: 28137749
    6. The crystal structure of the rat Munc13-1 MUN domain now reveals an elongated, arch-shaped architecture formed by alpha-helical bundles, with a highly conserved hydrophobic pocket in the middle. PMID: 26030875
    7. The C-terminal module of Munc13-1 is important for Munc13 function, but full activity requires adjacent modules. PMID: 22000513
    8. The Munc13-1 MUN domain markedly accelerates the transition from the syntaxin-1-Munc18-1 complex to the SNARE complex. PMID: 21499244
    9. metabotropic glutamate receptor mGlu7 activates phospholipase C, translocates munc-13-1 protein, and potentiates glutamate release at cerebrocortical nerve terminals PMID: 20375012
    10. Data suggest that, during repeated action potentials, Ca(2+) and phosphatidylinositolphosphate binding to the Munc13 C(2)B domain potentiate synaptic vesicle exocytosis, thereby offsetting synaptic depression induced by vesicle depletion. PMID: 20154707
    11. Presynaptic silencing was accompanied by decreases in levels of the priming proteins Munc13-1 and Rim1. PMID: 20130189
    12. Munc13-1 regulates insulin exocytosis PMID: 12871971
    13. A conserved tryptophan residue occludes the diacylglycerol/phorbol ester-binding site in the munc13-1 C1 domain, and as a consequence, the munc13-1 C1 domain requires a considerable conformational change for ligand binding. PMID: 15667202
    14. Study shows that the Munc13-1 C(2)A domain homodimerizes, and that homodimerization competes with Munc13-1/RIM heterodimerization. PMID: 16732694
    15. Expression, purification and characterization of critical domains of Munc13-1 are reported. PMID: 17687497
    16. Munc13s may provide a template to assemble syntaxin-1/SNAP-25 heterodimers, leading to an acceptor complex for synaptobrevin PMID: 18201107
    17. These results suggest that Munc13-1 exists in pancreas islets during fetus development and its deficiency in the pancreas, as occurs in IUGR, was in accordance with decreased blood insulin level. PMID: 18787382

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  • 亞細胞定位：
    Cytoplasm. Cell membrane; Peripheral membrane protein. Cell junction, synapse, presynaptic cell membrane; Peripheral membrane protein. Cell junction, synapse, presynaptic active zone. Note=Translocated to the plasma membrane in response to phorbol ester binding.
  • 蛋白家族：
    Unc-13 family
  • 組織特異性：
    Expressed in brain, with highest levels in the olfactory bulb, striatum, cerebral cortex, hippocampus and cerebellum. Also expressed in pancreatic islet cells.
  • 數(shù)據(jù)庫鏈接：