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  4. Recombinant Rat Sodium-dependent serotonin transporter (Slc6a4), partial

Recombinant Rat Sodium-dependent serotonin transporter (Slc6a4), partial

  • 中文名稱：
    大鼠Slc6a4重組蛋白
  • 貨號：
    CSB-YP021702RA
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大鼠Slc6a4重組蛋白
  • 貨號：
    CSB-EP021702RA
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大鼠Slc6a4重組蛋白
  • 貨號：
    CSB-EP021702RA-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大鼠Slc6a4重組蛋白
  • 貨號：
    CSB-BP021702RA
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大鼠Slc6a4重組蛋白
  • 貨號：
    CSB-MP021702RA
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    Slc6a4Sodium-dependent serotonin transporter; SERT; 5HT transporter; 5HTT; Solute carrier family 6 member 4
  • 種屬：
    Rattus norvegicus (Rat)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    Serotonin transporter whose primary function in the central nervous system involves the regulation of serotonergic signaling via transport of serotonin molecules from the synaptic cleft back into the pre-synaptic terminal for re-utilization. Plays a key role in mediating regulation of the availability of serotonin to other receptors of serotonergic systems. Terminates the action of serotonin and recycles it in a sodium-dependent manner.
  • 基因功能參考文獻(xiàn)：
    1. Study assessed whether acute inescapable stress (IS) differentially affects fear extinction and extinction recall in 5-HTT(-/-) rats and wildtype controls. Surprisingly, IS experience improved fear extinction recall in 5-HTT(-/-) rats to the level of wildtype animals, while wildtypes were unaffected by this IS. PMID: 29233642
    2. Effects of early life stress and 5-HTT genotype at the molecular level could constitute early life stress x 5-HTT genotype interaction modulating the impact of stress on Edinger-Westphal-Ucn1 and dorsal raphe-5-HT neurons. PMID: 27826101
    3. In 5-HTT (-/-) rats, there was developmental delay, with decreased object recognition, compared to control animals. PMID: 27208789
    4. Study found deficits in intracortical inhibitory circuitry within the Sert-/- genotype reflected by: 1) a reduction in GABA/AMPA ratio in LIV excitatory neurons, 2) fewer functional soma-targeting inhibitory synapses onto cortical input layer IV excitatory neurons, 3) a decreased GABAA alpha1 receptor expression, 4) a depolarized EGABA in excitatory layer IV neurons and 5) a decrease in membrane KCC2 chloride extruder PMID: 28158484
    5. This study demonstrated that the SERT immunoreactivity in neuronal fibres within the area adjacent to the cortical contusion decreased after injury and decreased SERT mRNA and protein expression were in the area adjacent to the cerebral cortex. PMID: 27082144
    6. Increased expression of Sert is associated with visceral obesity. PMID: 26707058
    7. Small-animal positron emission tomography with 4-[18F]-ADAM showed that SERT is a biological target of resveratrol PMID: 26612383
    8. These results indicated that SERT may play a critical role in the pathogenesis of hippocampal autophagy, and is likely involved in the development of PTSD. PMID: 26723990
    9. we found that SERT function is selectively enhanced in the frontal cortex, independently of the p38 MAPK pathway PMID: 26924154
    10. Disrupted fear acquisition and concomitant increase in contextual conditioned fear-potentiated startle fear in SERT(-/-) resembles suggests that normal SERT functioning is crucial for the development of an adequate fear neuro-circuitry PMID: 26302762
    11. SERT was identified in the blood brain barrier of male rats. PMID: 26223876
    12. Report altered serotonin transporter expression in brain after prenatal alcohol exposure. PMID: 26180184
    13. Altered regulation of the expression of CRF in these areas represents a major neurobiological mechanism underlying the interaction of early life stress and 5-HTT gene variation. PMID: 25640835
    14. Results suggest serotonergic activation in the dorsal raphe nucleus is involved in stress coping in a 5-HTT expression dependent manner, medial prefrontal cortex activation seems to be implicated in control over stress independently of 5-HTT expression PMID: 26132384
    15. caudal region of both ventral and lateral subnuclei, the level of 5-HTT expression was significantly higher than in the rostral region PMID: 26987216
    16. SERT does not play an essential role in the pathogenesis of severe angioobliterative pulmonary hypertension in rats exposed to Sugen hypoxia. PMID: 26386116
    17. both SERT deletion and the maternal separation (MS) led to an overall reduction in Bdnf expression in the ventral hippocampus and in the ventromedial prefrontal cortex PMID: 25087780
    18. Increased gene and protein expression of 5HT2A and 5-HTT in the pulmonary vasculature of nitrofen-induced CDH lungs suggest that 5HT2A and 5-HTT are important mediators of PH in nitrofen-induced CDH. PMID: 24888825
    19. Data show that SERT-/- rats are more sensitive to the initial, and subsequent, reinforcing properties of MDMA PMID: 23711978
    20. The high conditioned stimulus sensitivity in 5-HTT(-/-) rats cannot be explained by enhanced incentive salience attribution to the conditioned stimulus as measured by the sign- versus goal-tracking paradigm. PMID: 24269496
    21. These results suggest that an impairment of SERT may affect BDNF expression throughout postnatal development. PMID: 23564488
    22. This study demonistrated that serotonin transporter knockout mice showing affective disorder and treatment by lurasidone. PMID: 23164505
    23. Increased colonic extracellular serotonin in female SERT KO rats is associated with visceral hypersensitivity and hyperexcitability of colon projecting sensory neurons. PMID: 23594365
    24. an intact SERT function is needed for pro-inflammatory cytokine-induced anhedonia and weight loss in rats PMID: 23274951
    25. Peripubertally stressed rats showed increased 5HTT mRNA levels in the pre-frontal cortex. PMID: 23321813
    26. Neuronal sertonin transporter is the primary regulator of synaptic serotonin availability in the intact and hypoxia-ischemia-injured neonatal brain. PMID: 22525280
    27. Anxiety-related behavior in the social interaction test does not affect slc6a4 mRNA expression in dorsal raphe nucleus subregions. PMID: 22511363
    28. we show here that 5-HTT-/- rats outperformed 5-HTT+/+ rats in an extradimensional strategy set-shifting procedure PMID: 22505721
    29. Total 5HT and 5HT(2A) receptor number was increased in hypoxic neonates along with an up regulation of 5HT(2A) receptor and 5HT transporter gene. PMID: 21683764
    30. These results are the first to indicate a role for genetic variation in the 5-HTT gene in physiology and behavior in the rat. PMID: 21463019
    31. This article presents an association between the serotonin transporter gene polymorphism (5-HTTLPR) and pain modulation derived by nonpainful conditioned pain modulation. PMID: 21719351
    32. a conserved asparagine residue in transmembrane segment 1 (TM1) of serotonin transporter dictates chloride-coupled neurotransmitter transport PMID: 21730057
    33. 5-HTT-/- rats - which show increased anxiety and reversal learning under undisturbed conditions - became less anxious and flexible, 5-HTT+/+ rats responded more strongly to construction stress and displayed the opposite pattern. PMID: 21549766
    34. SERT function, when challenged with 5-HT, is modestly more important in the vasculature of the female compared with male rat. PMID: 21371073
    35. These findings provide evidence of a physical and compartmentalized association between SERT and PKGIalpha that supports. PMID: 19656393
    36. The data show for the first time an important interactive relationship between SERT and the PDGFRbeta in the production of pulmonary artery smooth muscle cell proliferation triggered by PDGF. PMID: 21186266
    37. The effects of severe early ID on emotional problems in adolescence are moderated by 5HTT genotype, and influenced by stressful life events in adolescence. PMID: 20345836
    38. 5-HTT(-/-) rats are unable to use the value of expected outcomes to guide behaviour, suggesting a new modulatory role of 5-HT in cognitive control over behaviour, which may have important implications for psychopathologies PMID: 20388545
    39. We found that BDNF levels were significantly reduced in the hippocampus and prefrontal cortex of SERT knockout rats, through transcriptional changes that affect different neurotrophin isoforms. PMID: 20034565
    40. Data indicate that selected derivatives showed high in vitro potency and selectivity at SERT, NET, and DAT. PMID: 20170186
    41. Data showed that multiple brainstem nuclei exhibited a significant decline in serotonin transporter immunoreactivity during postnatal days 2-21. PMID: 20127812
    42. serotonin transporter deficiency affects abdominal fat deposition in a sex-dependent way, but protects against rises in glucose levels, and thereby potentially glucose intolerance. PMID: 19444235
    43. This review concludes that SERT function is conserved across mice and rats and that their behavioral profile arises from common neurodevelopmental alterations. PMID: 19698744
    44. in the presence of low leptin levels, while the SERT does not appear to play a role, the activation and reduction of the NPY and POMC systems, respectively, may be factors involved in the triggering of rebound hyperphagia, induced by fasting PMID: 19968967
    45. Given its resemblance with the human SERT-linked polymorphic region and propensity to develop depression-like symptoms, our findings may contribute to further understanding of mechanisms underlying cognitive deficits in depression. PMID: 19740092
    46. rat 5HTT mRNA is alternatively spliced, leading to three distinct mRNAs differing in the 5' untranslated region; the third splice variant exon 1C is only expressed in the gut and to a lesser extent in the heart and may utilize a distinct promoter PMID: 12124434
    47. 6-OHDA lesions increased 5-HT transporter binding in the neostriatum and nucleus accumbens, but not cerebral cortex. Effects reached maximal levels at postnatal day 24, and were sustained until early adulthood PMID: 12220705
    48. Important role for CA3-serotonergic innervation in pentylenetetrazol-induced kindling of seizures. PMID: 12457739
    49. sialylated N-glycans provide a favorable conformation to serotonin transporter that allows the transporter to function most efficiently via its protein-protein interactions PMID: 12944413
    50. decreases in tissue 5-HT and serotonin transporter binding sites induced by D-FEN and parachloroamphetamine reflect neuroadaptive changes, rather than neurotoxic effects. PMID: 14515341

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  • 亞細(xì)胞定位：
    Cell membrane; Multi-pass membrane protein. Endomembrane system; Multi-pass membrane protein. Endosome membrane; Multi-pass membrane protein. Cell junction, synapse. Cell junction, focal adhesion.
  • 蛋白家族：
    Sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family, SLC6A4 subfamily
  • 數(shù)據(jù)庫鏈接：