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  4. Recombinant Saccharomyces cerevisiae DNA repair and recombination protein PIF1 (PIF1), partial

Recombinant Saccharomyces cerevisiae DNA repair and recombination protein PIF1 (PIF1), partial

  • 中文名稱：
    釀酒酵母PIF1重組蛋白
  • 貨號(hào)：
    CSB-YP362094SVG
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母PIF1重組蛋白
  • 貨號(hào)：
    CSB-EP362094SVG
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母PIF1重組蛋白
  • 貨號(hào)：
    CSB-EP362094SVG-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母PIF1重組蛋白
  • 貨號(hào)：
    CSB-BP362094SVG
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母PIF1重組蛋白
  • 貨號(hào)：
    CSB-MP362094SVG
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    PIF1
  • Uniprot No.：
  • 別名：
    PIF1; TST1; YML061C; YM9958.01C; ATP-dependent DNA helicase PIF1; EC 3.6.4.12; DNA repair and recombination helicase PIF1; Petite integration frequency protein 1; Telomere stability protein 1
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability. Efficiently unwinds G-quadruplex (G4) DNA structures and forked RNA-DNA hybrids. Appears to move along DNA in single nucleotide or base pair steps, powered by hydrolysis of 1 molecule of ATP. Processes at an unwinding rate of about 75 bp/s. Resolves G4 structures, preventing replication pausing and double-strand breaks (DSBs) at G4 motifs. Involved in the maintenance of telomeric DNA. Inhibits telomere elongation, de novo telomere formation and telomere addition to DSBs via catalytic inhibition of telomerase. Reduces the processivity of telomerase by displacing active telomerase from DNA ends. Releases telomerase by unwinding the short telomerase RNA/telomeric DNA hybrid that is the intermediate in the telomerase reaction. Involved in the maintenance of ribosomal (rDNA). Required for efficient fork arrest at the replication fork barrier within rDNA. Involved in the maintenance of mitochondrial (mtDNA). Required to maintain mtDNA under conditions that introduce dsDNA breaks in mtDNA, either preventing or repairing dsDNA breaks. May inhibit replication progression to allow time for repair. May have a general role in chromosomal replication by affecting Okazaki fragment maturation. May have a role in conjunction with DNA2 helicase/nuclease in 5'-flap extension during Okazaki fragment processing.
  • 基因功能參考文獻(xiàn)：
    1. Dna2 processes behind the fork long ssDNA flaps generated by Pif1 and replication-dependent strand displacement. PMID: 30446656
    2. It has been proposed that Rrm3 and Pif1 promote genome stability at tDNAs by displacing the stable multi-protein transcription complex and by removing R-loops. PMID: 28429714
    3. we provide in vivo evidence that Pif1 inhibits telomerase activity at DNA ends regardless of telomere sequence length. PMID: 29052759
    4. Multiple Pif1 helicases are required to sequentially disrupt G-quadruplex structure and unwind duplex DNA PMID: 27079238
    5. Either Pif1 or Rrm3 redundantly stimulates strand displacement by DNA polymerase delta during lagging-strand synthesis. PMID: 27991904
    6. The DNA unwinding activity of Pif1 can be regulated by force. PMID: 27098034
    7. Pif1 not only stimulates the strand displacement activity of Pol delta but it also allows efficient replication through the Rap1 block, by removing bound Rap1 in front of the polymerase. PMID: 27001517
    8. In budding and fission yeasts, Pif1 family helicases impact both telomerase-mediated and semi-conservative replication of telomeric DNA as well as recombination-mediated telomere lengthening. (Review) PMID: 27233114
    9. Using a DNA polymerase coupled assay and FRET (Forster resonance energy transfer)-based helicase assays, in this work, the authors show that a monomer of Saccharomyces cerevisiae Pif1 can unwind double-stranded DNA. PMID: 26908222
    10. Pif1 is capable of unwinding RNA:DNA heteroduplexes with moderately greater processivity compared with its duplex DNA:DNA counterparts. PMID: 26733194
    11. Rad53-mediated regulation of Rrm3 and Pif1 DNA helicases contributes to prevention of aberrant fork transitions under replication stress. PMID: 26411679
    12. The role of Pif1 in break induced replication and telomere lengthening:break-induced replication requires DNA damage-induced phosphorylation of Pif1 and leads to telomere lengthening. PMID: 25329304
    13. preferential lengthening of short yeast telomeres is achieved in part by targeting the negative regulator Pif1 to long telomeres PMID: 25906395
    14. periodic patrolling activity may keep Pif1 at its site of in vivo action in displacing telomerase, resolving R-loops, and keeping G4 unfolded during replication, recombination and repair PMID: 24843019
    15. Pif1 helicase unfolds G-quadruplexes in sequential and repetitive steps. PMID: 25471447
    16. Pif1 can anneal DNA in the presence of ATP and Mg(2+). Pif1-mediated annealing also occurs in the presence of single-stranded DNA binding proteins. PMID: 25393406
    17. The enhanced telomerase removal efficiency by Pif1 at the longer single-stranded telomeric DNA suggests a way of how Pif1 regulates telomerase activity and maintains telomere length. PMID: 24981509
    18. Pif1 stimulates DNA synthesis during break-induced replication and crossover recombination PMID: 24025768
    19. Results show that monomers of Pif1 are able to translocate on single-stranded DNA with 5' to 3' directionality. PMID: 23446274
    20. The data suggests that G4 structures form in vivo and that they are resolved by Pif1 to prevent replication fork stalling and DNA breakage. PMID: 21620135
    21. Pif1p is not only bound to mitochondrial DNA but also to the inner mitochondrial membrane either directly or indirectly via a protein complex. PMID: 20655619
    22. Pif1 removes Okazaki fragments initiated by fold-back flaps in vivo PMID: 20959454
    23. it is postulated that lack of Pif1 forces aconitase to play its DNA protective role as a nucleoid protein and that this triggers a domino effect on iron homoeostasis resulting in increased zinc tolerance PMID: 20858222
    24. Strains lacking Pif1p exhibit an increased rate of formation of petite mutants, an indicator of mtDNA instability, and elevated mtDNA point mutagenesis. PMID: 15907372
    25. Pif1p helicase activity limits telomerase action both in vivo and in vitro by displacing active telomerase from DNA ends PMID: 16121131
    26. Has a direct role in the prevention or repair of Sgs1-induced DNA damage that accumulates in top3 mutants. PMID: 16816432
    27. Pif1p participates in the protection from double-stranded (ds) DNA breaks or alternatively in the repair process of double-stranded DNA breaks in mtDNA. PMID: 17257907
    28. Forked RNA-DNA substrates are the favored substrates for Pif1p in vitro. PMID: 17720711
    29. Pif1 directs long flaps toward the two-nuclease pathway, requiring Dna2 cleavage for primer removal. PMID: 18689797
    30. Pif1p acts as a DNA helicase in mitochondria affects mtDNA replication directly and independently of the DNA helicase Rrm3p PMID: 19277716
    31. CEB1 repeats formed stable G-quadruplex (G4) secondary structures, and the Pif1 protein unwinds these structures more efficiently than regular B-DNA. PMID: 19424434
    32. Pif1 helicase lengthens some Okazaki fragment flaps necessitating Dna2 nuclease/helicase action in the two-nuclease processing pathway PMID: 19605347

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  • 亞細(xì)胞定位：
    [Isoform Nuclear]: Nucleus, nucleolus.; [Isoform Mitochondrial]: Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.
  • 蛋白家族：
    Helicase family, PIF1 subfamily
  • 數(shù)據(jù)庫鏈接：

    KEGG: sce:YML061C

    STRING: 4932.YML061C