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  4. Recombinant Saccharomyces cerevisiae DNA repair protein RAD9 (RAD9), partial

Recombinant Saccharomyces cerevisiae DNA repair protein RAD9 (RAD9), partial

  • 中文名稱：
    釀酒酵母RAD9重組蛋白
  • 貨號(hào)：
    CSB-YP320420SVG1
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母RAD9重組蛋白
  • 貨號(hào)：
    CSB-EP320420SVG1
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母RAD9重組蛋白
  • 貨號(hào)：
    CSB-EP320420SVG1-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母RAD9重組蛋白
  • 貨號(hào)：
    CSB-BP320420SVG1
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母RAD9重組蛋白
  • 貨號(hào)：
    CSB-MP320420SVG1
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    RAD9
  • Uniprot No.：
  • 別名：
    RAD9; YDR217C; YD9934.02C; DNA repair protein RAD9
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker\\\'s yeast)
  • 蛋白長(zhǎng)度：
    Partial
  • 表達(dá)區(qū)域：
    990-1309aa
  • 氨基酸序列
    SGEIRTGNVFDKCIFVLTSLFENREELRQTIESQGGTVIESGFSTLFNFTHPLAKSLVNKGNTDNIRELALKLAWKPHSLFADCRFACLITKRHLRSLKYLETLALGWPTLHWKFISACIEKKRIVPHLIYQYLLPSGESFRLSLDSPSKGGIIKSNNIFSFYTQFLRGSNLRDQICGVKKMLNDYIVIVWGRSELDSFVKFAFACLSAGRMLTIDLPNIDVDDTEPLLNALDSLVPRIGSELSNRKLKFLIYANENNGKSQMKLLERLRSQISLKFKKFNYIFHTESKEWLIQTIINEDTGFHDDITDNDIYNTISEVR
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Essential for cell cycle arrest at the G2 stage following DNA damage by X-irradiation or inactivation of DNA ligase.
  • 基因功能參考文獻(xiàn)：
    1. The initial telomere-proximal unstable chromosome is increased in mutants in telomerase subunits, Tel1, and even Rad9, with no known telomere-specific function. Defects in Tel1 and in Rrm3, a checkpoint protein kinase with a role in telomere maintenance and a DNA helicase, respectively, synergize dramatically to generate unstable chromosomes, further illustrating the consequence of replication error in the telomere. PMID: 27716774
    2. The checkpoint protein Rad9 limits the action of Sgs1/Dna2 in double-strand break resection by inhibiting Sgs1 binding/persistence at the double-strand break ends. PMID: 25637499
    3. in cells lacking Sae2, Rad9/53BP1 contributes to keep Mre11 bound to a persistent DSB, protecting it from extensive DNA end resection, which may lead to potentially deleterious DNA deletions and genome rearrangements. PMID: 25569305
    4. The findings suggest that Rad9 functions together with Aft1 on DNA damage-prone chromatin to facilitate genome surveillance, thereby ensuring rapid and effective response to possible DNA damage events. PMID: 25300486
    5. We suggest a novel model for Chk1 activation where Cdc28 regulates the constitutive interaction of Rad9 and Chk1. The Rad9/Chk1 complex is then recruited at sites of DNA damage where activation of Chk1 requires additional DDR-specific protein kinases. PMID: 23593009
    6. Multiple phosphorylation of Rad9 by the cyclin-dependent kinase is required for DNA damage checkpoint activation. PMID: 23070520
    7. Slx4-Rtt107 complex modulates Rad53 activation by a competition-based mechanism that balances the engagement of Rad9 at replication-induced lesions PMID: 23160493
    8. show that recombination intermediates take longer to form in cells lacking Rad9 PMID: 22484486
    9. a ternary complex of Dpb11, Mec1 and another key mediator protein Rad9 is required for efficient Rad9 phosphorylation by Mec1 in vitro, and for checkpoint activation in vivo. PMID: 21946560
    10. Rad9 chromatin binding is cell cycle-regulated by CDK1 activity. PMID: 20700441
    11. a novel role for members of the S-phase DNA damage checkpoint, RAD9, RAD17 and RAD24 in suppressing mutagenic post-replicative repair was described. PMID: 20472512
    12. report that Rad9 binds to sub-telomeric chromatin, upon telomere uncapping, up to 10 kb from the telomere. PMID: 19880356
    13. Rad9 acts as a bona fide signaling adaptor that enables Rad53 phosphorylation by Mec1. PMID: 16085488
    14. Dot1-dependent histone H3 methylation in G1 and S phase DNA damage checkpoint functions of Rad9 PMID: 16166626
    15. Rad9 has a role in promoting efficient repair of DNA DSBs by homologous recombination PMID: 16650810
    16. These findings indicate that constitutive Tudor domain-mediated and damage-specific BRCT domain-phospho-H2A-dependent interactions of Rad9 with chromatin cooperate to establish G1 checkpoint arrest. PMID: 17721446
    17. The tandem tudor region of Rad9 directly interacts with single-stranded DNA and double-stranded DNAs of various lengths and sequences. PMID: 17726056
    18. Binding of the checkpoint protein Rad9, through its Tudor domain, to methylated histone H3-K79 inhibits resection at DSBs and uncapped telomeres. PMID: 18418382
    19. Interaction between checkpoint genes RAD9, RAD17, RAD24, and RAD53 involved in the determination of yeast Saccharomyces cerevisiae sensitivity to ionizing radiation PMID: 18727386
    20. checkpoint genes RAD9, RAD17, RAD24, and RAD53 belong to a single epistatic group designated RAD9 and govern the same pathway. Genes RAD9 and RAD53 have a positive effect on sensitivity to gamma-radiation, whereas RAD17 and RAD24 have a negative effect PMID: 18825953
    21. the role of the histone H3K79 methyltransferase Dot1, Rad9 checkpoint adaptor in the cohesin-dependent repair by sister chromatid recombination of replication-dependent HO-induced double-strand break PMID: 19332880
    22. RAD9-dependent gene up-regulation is required for nucleotide excision repair only when this process is coupled to transcription. PMID: 19428371

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  • 亞細(xì)胞定位：
    Nucleus.
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: sce:YDR217C

    STRING: 4932.YDR217C