Recombinant Saccharomyces cerevisiae FACT complex subunit SPT16 (SPT16), partial
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中文名稱:釀酒酵母SPT16重組蛋白
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貨號:CSB-YP022955SVG
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規(guī)格:
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來源:Yeast
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其他:
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中文名稱:釀酒酵母SPT16重組蛋白
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貨號:CSB-EP022955SVG
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規(guī)格:
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來源:E.coli
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其他:
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中文名稱:釀酒酵母SPT16重組蛋白
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貨號:CSB-EP022955SVG-B
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規(guī)格:
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來源:E.coli
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共軛:Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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其他:
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中文名稱:釀酒酵母SPT16重組蛋白
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貨號:CSB-BP022955SVG
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規(guī)格:
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來源:Baculovirus
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其他:
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中文名稱:釀酒酵母SPT16重組蛋白
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貨號:CSB-MP022955SVG
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規(guī)格:
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來源:Mammalian cell
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其他:
產(chǎn)品詳情
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純度:>85% (SDS-PAGE)
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基因名:SPT16
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Uniprot No.:
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別名:SPT16; CDC68; SSF1; YGL207W; FACT complex subunit SPT16; Cell division control protein 68; Facilitates chromatin transcription complex subunit SPT16; Suppressor of Ty protein 16
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種屬:Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
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蛋白長度:Partial
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蛋白標簽:Tag?type?will?be?determined?during?the?manufacturing?process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially. -
產(chǎn)品提供形式:Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
復溶:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
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儲存條件:Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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保質(zhì)期:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期:Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
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注意事項:Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Datasheet :Please contact us to get it.
相關產(chǎn)品
靶點詳情
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功能:Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. Transcription elongation is promoted by the repression of transcription initiation from cryptic sites. Also acts in establishing transcription initiation complexes and promotes SPT15/TBP-binding to a TATA box. Together with replication factor-A protein (RPA), FACT may play a role in nucleosome deposition during DNA replication.
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基因功能參考文獻:
- SPT16 interactions with nucleosome. PMID: 29271283
- Results show that Spt16 depletion results in global changes in nucleosome fuzziness and position shifts and that the majority of these changes are suppressed by inhibiting RNA polymerase II supporting a model in which Spt16 has a role in maintaining local nucleosome stability. PMID: 27141053
- These results suggest that the large scale overlap in Mot1 and Spt16 function arises from a combination of both their unique and shared functions in transcription complex assembly and chromatin structure regulation. PMID: 27226635
- Spt6 and FACT have both overlapping and distinct functional interactions with histones and nucleosomes and that binding to each of the components of a nucleosome is not sufficient to produce nucleosome reorganization PMID: 26416482
- results suggest that the discrimination of Spt16 to Htz1-bound genes is due to the priority of Chz1 over Spt16 in binding to the Htz1-bound genomic regions; Chz1-escorted Htz1 therefore impairs Spt16 binding at chromatin PMID: 24707933
- The Spt16 subunit of FACT is specifically required for H2B deposition onto rDNA genes. PMID: 23689130
- Structure of the Spt16 middle domain reveals functional features of the histone chaperone FACT. PMID: 23417676
- Mutation that encodes the Spt16 subunit of FACT causes phenotypes associated with defects in transcription and replication. PMID: 21625001
- These findings identify Spt16 as an Rtt101 substrate, and suggest that Spt16 ubiquitylation is important for FACT to function during DNA replication. PMID: 20634314
- The FACT subunit Spt16 and Pkc1 signaling have an overlapping essential function, with an unexpected role for FACT in the maintenance of cell integrity. PMID: 19727824
- N-terminal region of Polalpha (Pol1p) that is evolutionarily conserved among different species interacts with Spt16p-Pob3p and Ctf4p in vivo PMID: 15485923
- The Spt16 N-terminal domain does become important under conditions of replication stress, where it mediates adaptation to stress conditions. PMID: 15520471
- Chd1 and SPT16 and POB3 act in opposition in regulating transcription. PMID: 17620414
- neither the Spt16 NTD nor a key residue in the putative Pob3-M-binding site was required for interactions with histone N termini or for yFACT-mediated nucleosome reorganization PMID: 18089575
- Spt16 restores normal nucleosome structure by redepositing the displaced H3-H4 histones, thereby preventing incorporation of new histones. PMID: 19683500
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亞細胞定位:Nucleus. Chromosome. Note=Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci.
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蛋白家族:Peptidase M24 family, SPT16 subfamily
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數(shù)據(jù)庫鏈接:
KEGG: sce:YGL207W
STRING: 4932.YGL207W
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