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  4. Recombinant Saccharomyces cerevisiae Homothallic switching endonuclease (HO), partial

Recombinant Saccharomyces cerevisiae Homothallic switching endonuclease (HO), partial

  • 中文名稱：
    釀酒酵母HO重組蛋白
  • 貨號：
    CSB-YP357811SVG
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母HO重組蛋白
  • 貨號：
    CSB-EP357811SVG
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母HO重組蛋白
  • 貨號：
    CSB-EP357811SVG-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母HO重組蛋白
  • 貨號：
    CSB-BP357811SVG
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母HO重組蛋白
  • 貨號：
    CSB-MP357811SVG
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    HO
  • Uniprot No.：
  • 別名：
    HO; YDL227CHomothallic switching endonuclease; Ho endonuclease
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Initiation of mating type interconversion. This protein is a site-specific endonuclease that cleaves a site in the mat locus on chromosome III. The double-strand break is followed by a unidirectional gene conversion event that replaces the information at the mat locus by information copied from either of the two homologous loci (HMR and HML) that reside at the extremity of the chromosome III. Endonuclease expression takes place in late G1 just before cells enter S phase.
  • 基因功能參考文獻：
    1. HO promotor region fires in an "on/off" fashion in WT cells as well as in different genetic backgrounds. PMID: 23836672
    2. Analysis of MATalpha2 and MATa1 binding sites on the HO promotor that mediate repression of the HO gene. PMID: 15598821
    3. DDI1 participates in MEC1-mediated degradation of HO. PMID: 15964793
    4. ho endonuclease nuclear import utilizes two nuclear localization signals and four importins of the ribosomal import system PMID: 16507575
    5. SWI/SNF, either directly or indirectly, facilitates TATA Binding Protein binding at HO. PMID: 16705163
    6. Nuclear export of Ho endonuclease of yeast via Msn5 PMID: 18807043
    7. Data show that coactivators bind first to far upstream promoter regions of HO and subsequently to a promoter proximal region, and FACT and Asf1 are both required for this coactivator re-recruitment. PMID: 19481521

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  • 亞細胞定位：
    Nucleus.
  • 數(shù)據(jù)庫鏈接：

    KEGG: sce:YDL227C

    STRING: 4932.YDL227C