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  4. Recombinant Saccharomyces cerevisiae Origin recognition complex subunit 5 (ORC5)

Recombinant Saccharomyces cerevisiae Origin recognition complex subunit 5 (ORC5)

  • 中文名稱：
    釀酒酵母ORC5重組蛋白
  • 貨號(hào)：
    CSB-YP017235SVG
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母ORC5重組蛋白
  • 貨號(hào)：
    CSB-EP017235SVG
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母ORC5重組蛋白
  • 貨號(hào)：
    CSB-EP017235SVG-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母ORC5重組蛋白
  • 貨號(hào)：
    CSB-BP017235SVG
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母ORC5重組蛋白
  • 貨號(hào)：
    CSB-MP017235SVG
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    ORC5; YNL261W; N0834; Origin recognition complex subunit 5; Origin recognition complex 53 kDa subunit
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長(zhǎng)度：
    full length protein
  • 表達(dá)區(qū)域：
    1-479
  • 氨基酸序列
    MNVTTPEVAF REYQTNCLAS YISADPDITP SNLILQGYSG TGKTYTLKKY FNANPNLHAV WLEPVELVSW KPLLQAIART VQYKLKTLYP NIPTTDYDPL QVEEPFLLVK TLHNIFVQYE SLQEKTCLFL ILDGFDSLQD LDAALFNKYI KLNELLPKDS KINIKFIYTM LETSFLQRYS THCIPTVMFP RYNVDEVSTI LVMSRCGELM EDSCLRKRII EEQITDCTDD QFQNVAANFI HLIVQAFHSY TGNDIFALND LIDFKWPKYV SRITKENIFE PLALYKSAIK LFLSTDDNLS ENGQGESAIT TNRDDLENSQ TYDLSIISKY LLIASYICSY LEPRYDASIF SRKTRIIQGR AAYGRRKKKE VNPRYLQPSL FAIERLLAIF QAIFPIQGKA ESGSLSALRE ESLMKANIEV FQNLSELHTL KLIATTMNKN IDYLSPKVRW KVNVPWEIIK EISESVHFNI SDYFSDIHE
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Component of the origin recognition complex (ORC) that binds origins of replication. It has a role in both chromosomal replication and mating type transcriptional silencing. Binds to the ARS consensus sequence (ACS) of origins of replication. This subunit is a candidate for the mediation of ATP-dependent binding of ORC to origins. May also be a substrate targeting component of a cullin-RING-based E3 ubiquitin-protein ligase complex RTT101(MMS1-ORC5).
  • 基因功能參考文獻(xiàn)：
    1. our findings suggest a role of Rrm3, via its Orc5-binding domain, in restricting DNA synthesis that is genetically and physically separable from its established catalytic role in facilitating fork progression through replication blocks. PMID: 27923055
    2. interaction is mediated by the C-terminal region of Orc4p, and the N-terminal region of Orc5p PMID: 17107343
  • 亞細(xì)胞定位：
    Nucleus.
  • 蛋白家族：
    ORC5 family
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: sce:YNL261W

    STRING: 4932.YNL261W