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  4. Recombinant Saccharomyces cerevisiae Ribonuclease H (RNH1)

Recombinant Saccharomyces cerevisiae Ribonuclease H (RNH1)

  • 中文名稱：
    釀酒酵母RNH1重組蛋白
  • 貨號(hào)：
    CSB-YP312954SVG
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱：
    釀酒酵母RNH1重組蛋白
  • 貨號(hào)：
    CSB-EP312954SVG
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱：
    釀酒酵母RNH1重組蛋白
  • 貨號(hào)：
    CSB-EP312954SVG-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    釀酒酵母RNH1重組蛋白
  • 貨號(hào)：
    CSB-BP312954SVG
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱：
    釀酒酵母RNH1重組蛋白
  • 貨號(hào)：
    CSB-MP312954SVG
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    RNH1
  • Uniprot No.：
  • 別名：
    RNH1; YMR234W; YM9959.16; Ribonuclease H; RNase H; EC 3.1.26.4
  • 種屬：
    Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
  • 蛋白長(zhǎng)度：
    full length protein
  • 表達(dá)區(qū)域：
    1-348
  • 氨基酸序列
    MARQGNFYAV RKGRETGIYN TWNECKNQVD GYGGAIYKKF NSYEQAKSFL GQPNTTSNYG SSTHAGGQVS KPHTTQKRVH RRNRPLHYSS LTSSSACSSL SSANTNTFYS VKSNVPNIES KIFNNWKDCQ AYVKHKRGIT FKKFEDQLAA ENFISGMSAH DYKLMNISKE SFESKYKLSS NTMYNKSMNV YCDGSSFGNG TSSSRAGYGA YFEGAPEENI SEPLLSGAQT NNRAEIEAVS EALKKIWEKL TNEKEKVNYQ IKTDSEYVTK LLNDRYMTYD NKKLEGLPNS DLIVPLVQRF VKVKKYYELN KECFKNNGKF QIEWVKGHDG DPGNEMADFL AKKGASRR
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.
  • 基因功能參考文獻(xiàn)：
    1. Depleting endogenous RNase H activity impairs R-loop removal in Saccharomyces cerevisiae, causing DNA damage that occurs preferentially in the repetitive ribosomal DNA locus (rDNA). PMID: 27938663
    2. high-resolution mapping of mitotic recombination events throughout the yeast genome in diploid strains of Saccharomyces cerevisiae lacking RNase H1 (rnh1Delta), RNase H2 (rnh201Delta), or both RNase H1 and RNase H2 (rnh1Delta rnh201Delta) PMID: 26400613
    3. Data show that overexpression of a cellular RNase H, which degrades RNA in an RNA:DNA hybrid, completely suppressed the increase in Ty1 multimer formation in an rrm3 mutant. PMID: 19414561
  • 蛋白家族：
    RNase H family
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: sce:YMR234W

    STRING: 4932.YMR234W