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  4. Recombinant Salmonella typhimurium Negative modulator of initiation of replication (seqA)

Recombinant Salmonella typhimurium Negative modulator of initiation of replication (seqA)

  • 中文名稱：
    鼠傷寒沙門菌seqA重組蛋白
  • 貨號(hào)：
    CSB-YP745254SXB
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    鼠傷寒沙門菌seqA重組蛋白
  • 貨號(hào)：
    CSB-EP745254SXB
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    鼠傷寒沙門菌seqA重組蛋白
  • 貨號(hào)：
    CSB-EP745254SXB-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    鼠傷寒沙門菌seqA重組蛋白
  • 貨號(hào)：
    CSB-BP745254SXB
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    鼠傷寒沙門菌seqA重組蛋白
  • 貨號(hào)：
    CSB-MP745254SXB
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    seqA
  • Uniprot No.：
  • 別名：
    seqA; STM0697; Negative modulator of initiation of replication
  • 種屬：
    Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
  • 蛋白長(zhǎng)度：
    full length protein
  • 表達(dá)區(qū)域：
    1-180
  • 氨基酸序列
    MKTIEVDDEL YSYIASHTKH IGESASDILR RMLKFSATTQ PTASAVKGTP AAQPVAEAKP VNPVKDKVRA MRELLLSDEY AEQKKAVNRF MLILTTLYSL DHHAFAEATE SLHGRTRVYF AADEQTLLKN GNQTKPKHVP GTPYWVITNT NTGRKCSMIE HIMQSMQFPA ELIEKVCGTI
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Negative regulator of replication initiation, which contributes to regulation of DNA replication and ensures that replication initiation occurs exactly once per chromosome per cell cycle. Binds to pairs of hemimethylated GATC sequences in the oriC region, thus preventing assembly of replication proteins and re-initiation at newly replicated origins. Repression is relieved when the region becomes fully methylated.
  • 基因功能參考文獻(xiàn)：
    1. From gene mutation studies, seqA protein appears to regulate fatty acid & phospholipid composition of cell membranes. PMID: 20132031
    2. Data show that SeqA is unable to bind nonmethylated DNA, and std operon derepression in seqA mutants may result from unconstrained HdfR-mediated activation of std transcription. PMID: 18805972
  • 亞細(xì)胞定位：
    Cytoplasm.
  • 蛋白家族：
    SeqA family
  • 數(shù)據(jù)庫鏈接：

    KEGG: stm:STM0697

    STRING: 99287.STM0697