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  4. Recombinant Tomato bushy stunt virus RNA-directed RNA polymerase (ORF1), partial

Recombinant Tomato bushy stunt virus RNA-directed RNA polymerase (ORF1), partial

  • 中文名稱(chēng)：
    Recombinant Tomato bushy stunt virus RNA-directed RNA polymerase (ORF1) ,partial,Yeast
  • 貨號(hào)：
    CSB-YP323400TGA
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱(chēng)：
    Recombinant Tomato bushy stunt virus RNA-directed RNA polymerase (ORF1) ,partial,Yeast
  • 貨號(hào)：
    CSB-EP323400TGA
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱(chēng)：
    Recombinant Tomato bushy stunt virus RNA-directed RNA polymerase (ORF1) ,partial,Yeast
  • 貨號(hào)：
    CSB-EP323400TGA-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱(chēng)：
    Recombinant Tomato bushy stunt virus RNA-directed RNA polymerase (ORF1) ,partial,Yeast
  • 貨號(hào)：
    CSB-BP323400TGA
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱(chēng)：
    Recombinant Tomato bushy stunt virus RNA-directed RNA polymerase (ORF1) ,partial,Yeast
  • 貨號(hào)：
    CSB-MP323400TGA
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    ORF1
  • Uniprot No.：
  • 別名：
    ORF1; RNA-directed RNA polymerase; EC 2.7.7.48; Protein p92) [Cleaved into: Protein p33]
  • 種屬：
    Tomato bushy stunt virus (strain Cherry) (TBSV)
  • 蛋白長(zhǎng)度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    RNA-dependent RNA polymerase that plays an essential role in the virus replication.
  • 基因功能參考文獻(xiàn)：
    1. Conserved motifs in polymerase(p92) modulate genome replication, subgenomic transcription, and amplification of defective interfering RNAs. PMID: 25568204
    2. analysis of p33-Independent activation of a truncated p92 RNA-dependent RNA polymerase of Tomato bushy stunt virus in yeast cell-free extract PMID: 22933278
    3. Deletion of Ino2 and Ino4 in S. cerevisiae reduces TBSV RNA accumulation and inhibits the p33 and p92 activity. PMID: 21561636
    4. p33 interacts with p92 in vitro and that the interaction requires the S1 subdomain, whereas the S2 subdomain plays lesser function. PMID: 16242746
    5. An in vitro assay with the tombusvirus replicase indicated that Rpb11p affects TBSV replication and recombination only indirectly, via regulating p33 and p92(pol) levels. PMID: 17689583
    6. The in vitro assembly of the functional tombusvirus replicase required rATP and rGTP and the membranous fraction and Ssa1p (Hsp70). PMID: 19060219

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  • 蛋白家族：
    Tombusviridae RNA polymerase family
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: vg:1493955