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Recombinant Vaccinia virus mRNA-decapping protein D10 (VACWR115)

  • 中文名稱：
    Recombinant Vaccinia virus mRNA-decapping protein D10 (VACWR115),Yeast
  • 貨號(hào)：
    CSB-YP361248VAI
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Vaccinia virus mRNA-decapping protein D10 (VACWR115),Yeast
  • 貨號(hào)：
    CSB-EP361248VAI-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Vaccinia virus mRNA-decapping protein D10 (VACWR115),Yeast
  • 貨號(hào)：
    CSB-BP361248VAI
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Vaccinia virus mRNA-decapping protein D10 (VACWR115),Yeast
  • 貨號(hào)：
    CSB-MP361248VAI
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    VACWR115
  • Uniprot No.：
  • 別名：
    VACWR115; D10RmRNA-decapping protein D10; EC 3.1.3.-
  • 種屬：
    Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
  • 蛋白長度：
    full length protein
  • 表達(dá)區(qū)域：
    1-248
  • 氨基酸序列
    MNFYRSSIIS QIIKYNRRLA KSIICEDDSQ IITLTAFVNQ CLWCHKRVSV SAILLTTDNK ILVCNRRDSF LYSEIIRTRN MFRKKRLFLN YSNYLNKQER SILSSFFSLD PATADNDRID AIYPGGIPKR GENVPECLSR EIKEEVNIDN SFVFIDTRFF IHGIIEDTII NKFFEVIFFV GRISLTSDQI IDTFKSNHEI KDLIFLDPNS GNGLQYEIAK YALDTAKLKC YGHRGCYYES LKKLTEDD
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Decapping enzyme required for the removal of the 5'-end m7GpppN cap tethered to viral and host mRNAs to allow their decay in cells. May therefore accelerate viral and cellular mRNA turnover to eliminate competing host mRNAs and allow stage-specific synthesis of viral proteins. Acceleration of the turnover of cellular transcripts may even promote the shutoff of host protein synthesis.
  • 基因功能參考文獻(xiàn)：
    1. The non-canonical cap-recognition mode of the vaccinia virus D10 decapping enzyme is illustrated using a combination of nucleotide analogs, molecular modeling, and site-directed mutagenesis. PMID: 20639534
    2. demonstrate that a purified recombinant D10 fusion protein possesses an intrinsic activity that liberates m7GDP from capped RNA substrates. Furthermore, point mutations in the Nudix/MutT motif abolished decapping activity. PMID: 17283339
    3. IF I AM CORRECT THAT D10R IS NOT D10, THEN THE GENE LINK PLACED IN THIS RECORD IS PROBABLY INCORRECTLY LINKED. PMID: 19210265
  • 蛋白家族：
    Nudix hydrolase family
  • 數(shù)據(jù)庫鏈接：

    KEGG: vg:3707571