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Recombinant Mouse Rhodopsin (Rho)

  • 中文名稱：
    小鼠Rho重組蛋白
  • 貨號(hào)：
    CSB-CF019681MO
  • 規(guī)格：
  • 來源：
    in vitro E.coli expression system
  • 其他：

產(chǎn)品詳情

  • 基因名：
  • Uniprot No.：
  • 別名：
    Rho; Rhodopsin
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Full length protein
  • 表達(dá)區(qū)域：
    1-348
  • 氨基酸序列
    MNGTEGPNFYVPFSNVTGVVRSPFEQPQYYLAEPWQFSMLAAYMFLLIVLGFPINFLTLY VTVQHKKLRTPLNYILLNLAVADLFMVFGGFTTTLYTSLHGYFVFGPTGCNLEGFFATLG GEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVVFTWIMALACAAPPLVGWSRYIP EGMQCSCGIDYYTLKPEVNNESFVIYMFVVHFTIPMIVIFFCYGQLVFTVKEAAAQQQES ATTQKAEKEVTRMVIIMVIFFLICWLPYASVAFYIFTHQGSNFGPIFMTLPAFFAKSSSI YNPVIYIMLNKQFRNCMLTTLCCGKNPLGDDDASATASKTETSQVAPA
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標(biāo)簽：
    N-terminal 10xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change that activates signaling via G-proteins. Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling.
  • 基因功能參考文獻(xiàn)：
    1. Autosomal dominant retinitis pigmentosa rhodopsin mutant Q344X drives specific alterations in chromatin complex gene transcription. PMID: 29463953
    2. we show that OPN2 and OPN4 participate in immediate pigment darkening induced by UVA in murine normal and malignant melanocytes through a conserved common pathway PMID: 29395480
    3. Photoactivation of rhodopsin increases near-Infrared backscattering from rods and causes lengthening of their rod outer segment. PMID: 28320964
    4. Specific visible radiation facilitates lipolysis in mature 3T3-L1 adipocytes via rhodopsin-dependent beta3-adrenergic signaling. PMID: 28483278
    5. By modifying culture conditions in the SFEBq protocol, we obtained rod-dominated 3D retinas and S- and M-opsin expressing 3D retinas. PMID: 29274337
    6. Rab8a and Rab11a Are Dispensable for Rhodopsin Transport in Mouse Photoreceptors PMID: 27529348
    7. This study demonstrated that Rhodopsin Phosphorylation on Dark Adaptation in Mouse Rods. PMID: 27358455
    8. Findings indicate that Rho and ROCK knockout may improve the behavior of mice and prevent MPTP-induced dopaminergic neurons damage by regulating Sema3A, PlexinA and NRP-1 in a mouse model of Parkinson's disease. PMID: 27772760
    9. The authors elucidated this dependency by showing that guanylate cyclase-1 is a novel rhodopsin-binding protein. PMID: 26590321
    10. Eliminating Cngb1 and reducing RDS leads to additive defects in RDS expression levels and rod electroretinogram (ERG) function, (e.g., Cngb1-/-/rds+/- versus rds+/- or Cngb1-/-) but not to additive defects in rod ultrastructure. PMID: 26934134
    11. These findings reveal that an early and significant pathophysiologic effect of endoplasmic reticulum stress in photoreceptors is the highly efficient elimination of misfolded rhodopsin protein. PMID: 25270370
    12. Data show that G90D1 ribozyme efficiently and specifically cleaved the mutant transcript of the G90D mutation in the rhodopsin gene while G90D2 ribozyme cleaved both WT and mutant transcript. PMID: 26427453
    13. These results provide precise genotypic information of the P23H-1 rat with additional phenotypic characterization that will serve basis for therapeutic interventions, especially for those aiming at gene editing. PMID: 26009893
    14. Data show that misfolded opsin mutants form aggregates in the endoplasmic reticulum. PMID: 26358292
    15. Data show that the step-like responses of serine-only rhodopsin reflect slow and stochastic arrestin binding. PMID: 25910054
    16. Data indicate that genomic sequences from the rhodopsin gene can improve the efficacy of rhodopsin gene therapy in the rhodopsin knockout (RKO) mouse model of retinitis pigmentosa (RP). PMID: 25713057
    17. Peripherin-2 links CNGB1 to the light-detector rhodopsin in outer segments of rod photoreceptors. PMID: 24963162
    18. p27(kip1) promotes mesenchymal migration and hinders amoeboid migration upstream of the Rho/ROCK pathway. PMID: 25015295
    19. Data indicate that the number of nanodomains present in a single disc was dependent on the number of rhodopsin molecules incorporated into the membrane. PMID: 25305340
    20. mice deficient in the TWIK-2 channel develop pulmonary hypertension between 8 and 20 weeks of age through a mechanism involving Rho-kinase. PMID: 25245387
    21. P23H mutant Rho can trigger phototransduction but Rho P23H/P23H rods are 17,000-fold less sensitive to light than Rho +/+ rods and produce abnormally fast photo-responses. PMID: 24214395
    22. During development, some rhodopsin-expressing cells are displaced to the inner retinal layers. PMID: 24496510
    23. We created the T17M RHO CASP7 and T17M RHO CHOP mice to study the impact of the CASP7 or CHOP ablations in T17M RHO retina. PMID: 24664731
    24. We examine and compare the contribution of endoplasmic reticulum stress to retinal degeneration in several vertebrate models of retinitis pigmentosa generated through expression of mutant rhodopsins. PMID: 24664747
    25. rod outer segments lengthen and its rhodopsin concentration rises to increase photon capture in darker environment. PMID: 23985328
    26. The results reveal that the volume of the rod outer segment is proportional to rhodopsin gene expression; that P23H-rhodopsin, the most common rhodopsin gene disease allele, causes cell death via a dominant-negative mechanism PMID: 23185477
    27. This study demonistrated that the Muller glia express rhodopsin in a mouse model of inherited retinal degeneration. PMID: 22967839
    28. Autosomal recessive retinitis pigmentosa E150K opsin mice exhibit photoreceptor disorganization. PMID: 23221340
    29. Rhodopsin expression level affects rod outer segment morphology and photoresponse kinetics PMID: 22662234
    30. Stimulation of channel rhodopsin 2-containing fibers with millisecond flashes of blue light produces fast postsynaptic currents in tuberomammillary histamine neurons. PMID: 22956835
    31. analysis of structural, energetic, and mechanical perturbations in rhodopsin mutant that causes congenital stationary night blindness PMID: 22549882
    32. The endoplasmic reticulum stress response is involved in retinal degeneration in mice with rhodopsin mutation T17M PMID: 22589437
    33. A new mutation in rhodopsin was identifies in a mouse model of retinal degeneration. PMID: 22183357
    34. Adeno-associated virus delivery of wild-type rhodopsin preserves retinal function in a mouse model of autosomal dominant retinitis pigmentosa PMID: 21126223
    35. Role of bulk water in hydrolysis of the rhodopsin chromophore. PMID: 21460218
    36. The amino acid residues that differ naturally between mouse and bovine rhodopsin appear to have minimal bearing on molecular interactions stabilizing structural segments and unfolding intermediates; no major differences in unfolding energy are observed. PMID: 21038881
    37. High levels of retinal docosahexaenoic acid do not protect mice expressing the VPP rhodopsin mutation from retinal degeneration. PMID: 20806040
    38. Mutations of the opsin gene lead to light-induced degeneration of photoreceptors and constitutive activation of phototransduction. PMID: 20207741
    39. Rhodopsin phosphorylation has three physiological functions: it quenches phototransduction, reduces sensitivity during light adaptation, and suppresses bleached rhodopsin activity during dark adaptation. PMID: 20155952
    40. Progressive photoreceptor degeneration, outer segment dysplasia, and rhodopsin mislocalization in mice with targeted disruption of the retinitis pigmentosa-1 (Rp1) gene. PMID: 11960024
    41. Atomic-force microscopy of rhodopsin dimers in native disc membranes PMID: 12520290
    42. structure of rhodopsin and opsin dimer in native membranes PMID: 12663652
    43. Data describe the organization of the prototypical G protein-coupled receptor rhodopsin in its native membrane by electron and atomic force microscopy. PMID: 15111110
    44. Data suggest that the differences in physiological responses measured in wild type and rhodopsin knockout mice are due to structural changes of the whole rod outer segment and not due to a lower density of rhodopsin. PMID: 15337746
    45. palmitoylation may modulate rod photoreceptor sensitivity by permitting rhodopsin to remain active for a longer period PMID: 15851469
    46. rods generate reproducible single-photon responses; this reproducibility, consistency of amplitude & duration of rhodopsin activity, varies in a graded & systematic manner with the number but not identity of phosphorylation sites on rhodopsin's C terminus PMID: 16873665
    47. reveals how the molecular properties of rhodopsin affect the amplitude, shape, and kinetics of the rod response PMID: 17194706
    48. A single Rho molecule is necessary and sufficient to bind Arrestin. PMID: 17360618
    49. These experiments indicate that mutations of rhodopsin( Gly90Asp ) that lead to increases in cGMP and Ca(2+) can trigger photoreceptor degeneration. PMID: 17699662
    50. A STAT3-dependent E3 ubiquitin ligase, Ubr1, was responsible for rhodopsin degradation and was up-regulated in the inflamed SOCS3-deficient retinas. PMID: 18614536

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  • 亞細(xì)胞定位：
    Membrane; Multi-pass membrane protein. Cell projection, cilium, photoreceptor outer segment.
  • 蛋白家族：
    G-protein coupled receptor 1 family, Opsin subfamily
  • 組織特異性：
    Rod-shaped photoreceptor cells in the retina (at protein level).
  • 數(shù)據(jù)庫鏈接：