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Recombinant Mouse Sterol regulatory element-binding protein 2 (Srebf2)

  • 中文名稱：
    小鼠Srebf2重組蛋白
  • 貨號：
    CSB-CF666241MO
  • 規(guī)格：
  • 來源：
    in vitro E.coli expression system
  • 其他：

產(chǎn)品詳情

  • 基因名：
  • Uniprot No.：
  • 別名：
    Srebf2; Srebp2; Sterol regulatory element-binding protein 2; SREBP-2; Sterol regulatory element-binding transcription factor 2
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    full length protein
  • 表達(dá)區(qū)域：
    1-473
  • 氨基酸序列
    MDESSELGVLETMETLTELGDELTLGDIDEMLQFVSNQVGEFPDLFSEQLCSSFPGGGSN GGSGNNSSGRGNNGGATDPAVQRSFSQVPLSTFSPSAASPQAPALQVKVSPTPPRATPVL QPRPQPQPQPPAQLQQQTVMITPTFSTAPQTRIIQQPLIYQNAATSFQVLQPQVQSLVTS PQVQPVTIQQQVQTVQAQRVLTQTANGTLQTLAPATVQTVAAPQVQQVPVLVQPQIIKTD SLVLTTLKTDGSPVMAAVQNPALTALTAPIQTAALQVPTLVGSNGTILTTMPVMMGQEKV PIKQVPGGVKQLDPPKEGERRTTHNIIEKRYRSSINDKIIELKDLVMGTDAKMHKSGVLR KAIDYIKYLQQVNHKLRQENMVLKLANQKNKLLKGIDLGSLVDSDVDLKIDDFNQNVLLM SPPASDSGSQAGFSPYSIDSEPGSPLLDDAKVKDEPDSPPVALGMVDRSRILL
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標(biāo)簽：
    N-terminal 10xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Precursor of the transcription factor form (Processed sterol regulatory element-binding protein 2), which is embedded in the endoplasmic reticulum membrane. Low sterol concentrations promote processing of this form, releasing the transcription factor form that translocates into the nucleus and activates transcription of genes involved in cholesterol biosynthesis.; Key transcription factor that regulates expression of genes involved in cholesterol biosynthesis. Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3'). Regulates transcription of genes related to cholesterol synthesis pathway.
  • 基因功能參考文獻(xiàn)：
    1. Hexacosanol activates AMPK and hepatic autophagy and inhibits SREBP2, resulting in hypocholesterolemic activities and improvement of hepatic steatosis. PMID: 28676202
    2. increase in SREBP-2 directly activates expression of patatin-like phospholipase domain-containing enzyme 8 (PNPLA8) gene, and PNPLA8 associates with autophagosomes and is associated with a decrease in cellular triglyceride PMID: 27767079
    3. Reexpression of SCAP in SCAP-deficient cells restored SREBP2 protein expression and partially restored steroidogenic responses, confirming the requirement of SCAP-SREBP2 in steroidogenesis. PMID: 27601673
    4. Data, including data from studies using transgenic mice, suggest that oligodendroglial myelination requires astrocyte-derived lipids; oligodendrocyte-specific inactivation of sterol regulatory element-binding protein (SREBP) cleavage-activating protein (SCAP), an essential factor in lipid biosynthesis along with SREBP2, results in significantly retarded CNS myelination. PMID: 28549068
    5. The findings suggest that Cyp3a deficiency stimulated the expression of Scap via activation of the AR, which elevated cholesterogenic gene expression levels through activation of SREBP2 and increased total cholesterol contents in the prostate. PMID: 27137100
    6. SIRT1 gene knock-out may aggravate cartilage degeneration in osteoarthritis by activating the SREBP2 protein-mediated PI3K/AKT signalling pathway, suggesting that SIRT1 gene may play a protective role against osteoarthritis. PMID: 27752896
    7. Identify a novel signaling pathway in hepatocytes triggered by ligand-activated p75NTR that via p38 MAPK and caspase-3 mediate the activation of SREBP2. This pathway may regulate LDLRs and lipid uptake particularly after injury or during tissue inflammation accompanied by an increased production of growth factors, including NGF and pro-NGF. PMID: 26984409
    8. mTORC1/SREBP pathway is a major mechanism through which common oncogenic signaling events induce de novo lipid synthesis to promote aberrant growth and proliferation of cancer cells PMID: 26028026
    9. This study reveals SHP as a global transcriptional partner of SREBP-2 in regulation of sterol biosynthetic gene networks and provides a potential mechanism for cholesterol-lowering action of FGF19. PMID: 26634251
    10. activation of the sterol regulatory element binding protein-2 (SREBP2) was found to be downstream of ER stress, and this activation was affirmed to account for the intracellular accumulation of cholesterol using RNAi technique PMID: 26184741
    11. ITCH modulates SIRT6 and SREBP2 to influence lipid metabolism and atherosclerosis in ApoE null mice PMID: 25777360
    12. SREBP2-induced miR-92a targets key molecules in endothelial homeostasis, including sirtuin 1, Kruppel-like factor 2, and Kruppel-like factor 4, leading to NOD-like receptor family pyrin domain-containing 3 inflammasome activation and endothelial nitric oxide synthase inhibition. PMID: 25550450
    13. The cholesterol-lowering effects of FGF21 are abrogated by hepatic expression of sterol regulatory element-binding protein-2. PMID: 25794851
    14. Data indicate that Leishmania exploits macrophage cholesterol-dependent sterol regulatory element binding factor 2 (SREBP2) circuit to facilitate its entry and survival within the host. PMID: 25218172
    15. Demonstrate that the activation of FXR uncouples the expression of nuclear SREBP-2 and miR-33, and the regulation of their respective target genes. PMID: 25593129
    16. the cardiac sterol regulatory element-binding protein-2/3-hydroxy-3-methylglutaryl-coenzyme A reductase pathway being upregulated in MMP-2 deficiency. PMID: 25646300
    17. activation of intestinal SREBP2 alone seems to be sufficient to increase plasma cholesterol PMID: 24465397
    18. Hepatic insulin receptor knockout mice manifest decreased nuclear SREBP-2 protein, decreased cholesterologenic gene expression, and decreased cholesterol synthesis. PMID: 24516236
    19. The regulation of SREBP-2 in cholesterol transport is through activation of NF-kappaB. PMID: 24360166
    20. A vicious cycle in LDLR degradation might be generated by PCSK9 induced by hepatic Idol overexpression via dual mechanisms: sterol regulatory element-binding protein 2/LDLR. PMID: 24675665
    21. Inflammation disrupts LDLr feedback regulation through the activation of the mTOR pathway, increasing mTORC1 activity and up-regulating SREBP-2-mediated cholesterol uptake through Rb phosphorylation. PMID: 24068000
    22. Sirt6 plays a critical role in the regulation of hepatic Srebp2 gene expression and cholesterol homeostasis. PMID: 23881913
    23. A CYP7A1/SREBP2/miR-33a axis plays a critical role in regulation of hepatic cholesterol, bile acid, and fatty acid synthesis. PMID: 23536474
    24. Atheroprone flow induces NLRP3 inflammasome in endothelium through SREBP2 activation. PMID: 23838163
    25. Acetoacetyl-CoA synthetase, a ketone body-utilizing enzyme, is controlled by SREBP-2 and affects serum cholesterol levels. PMID: 22985732
    26. These results suggest that AACS is regulated by SREBP-2 and involves in the normal development of neurons. PMID: 23000407
    27. Data indicate that the induction of SREBP-2 and ER stress pathways is independent of PPARalpha activation in livers of newborn peroxisome assembly factor 1 (Pex2) knockout mice. PMID: 22441164
    28. Enlargement of the endoplasmic reticulum is independent of the SREBP-2 activation. PMID: 22675522
    29. Endoplasmic reticulum stress-induced SREBP-2 activation contributes to renal proximal tubule cell injury by dysregulating lipid homeostasis. PMID: 22573382
    30. the identified C3112T substitution in Srebf2 is responsible for the Lens opacity 13 phenotype PMID: 21858719
    31. Linalool reduces the expression of 3-hydroxy-3-methylglutaryl CoA reductase via sterol regulatory element binding protein-2- and ubiquitin-dependent mechanisms. PMID: 21944868
    32. SREBP-2 directly activates autophagy genes during cell-sterol depletion, conditions known to induce both autophagy and nuclear SREBP-2 levels. PMID: 21459322
    33. In insulin-deficient diabetic mice, a reduction in expression of SREBP-2 and its downstream genes in the hypothalamus and other areas of the brain, leads to a reduction in brain cholesterol synthesis and synaptosomal cholesterol content. PMID: 21109190
    34. The current data show that adipocyte hypertrophy and chronic inflammation are equally important in inducing chemerin synthesis. PMID: 21084441
    35. Activated sterol regulatory element-binding protein-2 (SREBP-2) interacts with peroxisome proliferator-activated receptor gamma coactivator (PGC-1alpha) in livers at reduced cholesterol intake. PMID: 20926756
    36. miR-33 expression from an SREBP2 intron inhibits cholesterol export and fatty acid oxidation PMID: 20732877
    37. Inflammation causes lipid accumulation in liver via disrupting SREBP-2 and LDLr expression. PMID: 20510003
    38. Data show that miR-33 is encoded within SREBP-2 and that both mRNAs are coexpressed. PMID: 20566875
    39. miR-33a/b embedded within introns of SREBP genes targets ABCA1, a regulator of HDL synthesis and reverse cholesterol transport, for posttranscriptional repression; indicates miR-33 acts in concert with SREBP host genes to control cholesterol homeostasis PMID: 20466882
    40. Results describe the characterization of a novel isoform of the transcription factor SREBP2, which is highly enriched in spermatogenic cells, is expressed in a stage-dependent fashion, and is not subject to feedback control by sterols. PMID: 12446768
    41. Thyroid hormone regulation and cholesterol metabolism are connected through SREBP-2 PMID: 12829694
    42. Sterol regulatory element-binding protein-2 interacts with hepatocyte nuclear factor-4 to enhance sterol isomerase gene expression in hepatocytes PMID: 12855700
    43. Lanosterol metabolism and SREBP2 expression in male germ cell maturation PMID: 12943732
    44. SREBP2gc is a trans-activator of male germ cell-specific gene expression. PMID: 15572673
    45. The SREBP2 protein is relatively specific to cholesterol synthesis. PMID: 15589694
    46. role in regulating mevalonate kinase and cob(I)alamin adenosyltransferase PMID: 17300749
    47. The amounts of sterol-dependent proprotein convertase subtilisin/kexin type 9 (Pcsk9) mRNA in livers of refed mice correlates with Srebp-2 regulation by the changes in the nuclear form of Srebp-2. PMID: 17921436
    48. SREBP2 regulates gut peptide secretion through intestineal bitter taste receptor signaling in mice. PMID: 18846256
    49. The induction of Srd5a2 through SREBP-2 provides a mechanistic explanation for why even though statin therapy is effective in reducing cholesterol levels in treating hypercholesterolemia it does not compromise androgen production in clinical studies. PMID: 19500568
    50. Up-regulation of cholesterogenic genes was attributable to a prion-dependent increased activity of the Srebp2, resulting in elevated levels of total and free cellular cholesterol. PMID: 19748890

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  • 亞細(xì)胞定位：
    Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Cytoplasmic vesicle, COPII-coated vesicle membrane; Multi-pass membrane protein.; [Processed sterol regulatory element-binding protein 2]: Nucleus.
  • 數(shù)據(jù)庫鏈接：