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Recombinant Rat Aquaporin-4 (Aqp4)

  • 中文名稱：
    Recombinant Rat Aquaporin-4(Aqp4)
  • 貨號：
    CSB-CF001964RA
  • 規(guī)格：
  • 來源：
    in vitro E.coli expression system
  • 其他：

產品詳情

  • 基因名：
  • Uniprot No.：
  • 別名：
    Aqp4; Aquaporin-4; AQP-4; Mercurial-insensitive water channel; MIWC; WCH4
  • 種屬：
    Rattus norvegicus (Rat)
  • 蛋白長度：
    Full length protein
  • 表達區(qū)域：
    1-323
  • 氨基酸序列
    MSDGAAARRWGKCGPPCSRESIMVAFKGVWTQAFWKAVTAEFLAMLIFVLLSVGSTINWGGSENPLPVDMVLISLCFGLSIATMVQCFGHISGGHINPAVTVAMVCTRKISIAKSVFYITAQCLGAIIGAGILYLVTPPSVVGGLGVTTVHGNLTAGHGLLVELIITFQLVFTIFASCDSKRTDVTGSVALAIGFSVAIGHLFAINYTGASMNPARSFGPAVIMGNWENHWIYWVGPIIGAVLAGALYEYVFCPDVELKRRLKEAFSKAAQQTKGSYMEVEDNRSQVETEDLILKPGVVHVIDIDRGDEKKGKDSSGEVLSSV
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標簽：
    N-terminal 10xHis-tagged
  • 產品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Forms a water-specific channel. Plays an important role in brain water homeostasis and in glymphatic solute transport. Required for a normal rate of water exchange across the blood brain interface. Required for normal levels of cerebrospinal fluid influx into the brain cortex and parenchyma along paravascular spaces that surround penetrating arteries, and for normal drainage of interstitial fluid along paravenous drainage pathways. Thereby, it is required for normal clearance of solutes from the brain interstitial fluid, including soluble beta-amyloid peptides derived from APP. Plays a redundant role in urinary water homeostasis and urinary concentrating ability.
  • 基因功能參考文獻：
    1. The hydrocephalus rat model was established successfully, and hydrocephalus rats showed a higher AQP4 level. Silencing AQP4 aggravated the hydrocephalus, with enlarged lateral ventricles and destruction of ependymal integrity and blood-brain barrier. PMID: 29921834
    2. These findings showed that eclamptic seizures induced cell death and that upregulation of AQP4 and AQP9 may play an important role in this pathophysiological process. PMID: 29351212
    3. HMGB1 upregulates AQP4 expression and promotes cell swelling in cultured spinal cord astrocytes after oxygen-glucose deprivation/reoxygenation PMID: 29178911
    4. down-regulation of AQP4 by lentiviral or shRNA vector reversed the brain edema and neurologic deficit induced by hypoxia-ischemia. PMID: 27726111
    5. Taken together, our results show that miR-145 plays an important role in protecting astrocytes from ischemic injury by downregulating AQP4 expression. These findings may highlight a novel therapeutic target in cerebral ischemic stroke. PMID: 29057271
    6. Interaction of the IgG-AQP4 complex with FcgammaRs triggers coendocytosis of the excitatory amino acid transporter 2. PMID: 28461494
    7. The diffusive and AQP4-independent solute transport in rodent brain parenchyma has been demonstrated. PMID: 28826498
    8. Lentivirus-mediated AQP4 inhibition accelerated motor function recovery after spinal cord injury, which was related to the up-regulation of NGF. This work reports the relationship between AQP4 and NGF in spinal cord injury for the first time. PMID: 28549966
    9. Spinal cord injury increases AQP4 expression in the spinal cord white matter and methylprednisolone diminishes this increase to baseline levels PMID: 28572712
    10. AQP4 forms protein complexes with GLT-1 and MOR in brain tissue and primary cultured astrocytes. Residues 252 to 323 is the site of AQP4 interaction with GLT-1. PMID: 28341892
    11. AQP4ex expression can be positively modulated by gentamicin treatment, demonstrating the possibility of regulating the AQP4 translational readthrough frequency PMID: 28206694
    12. Data suggest that AQP4 (aquaporin-4) and interleukin-1beta (IL1b) are involved in regulation of apoptosis; here, silencing AQP4 with small interfering RNA protects articular chondrocytes in culture from IL1b-induced apoptosis and stimulates cell proliferation. PMID: 29066483
    13. Results provide a novel, complement-dependent mechanism in neuromyelitis optica spectrum disorder by which AQP4-IgG binding to astrocytes injures surrounding oligodendrocytes, resulting in early and marked demyelination. PMID: 28567523
    14. Aquaporin 4 affects the dynamics of cell swelling and regulatory volume decrease in astrocytes exposed to hypo-osmotic conditions. PMID: 28978666
    15. The expressions of AQP4 were colocalized with GFAP. Protein levels of VEGF in the hyperglycemic rat retina were significantly higher than controls. FACS analyses showed that exposure to VEGF enlarged Muller cells, while exposure to TGN-020 suppressed the enlargement. PMID: 28303331
    16. interation of DG with laminin and dynamin is involved in the regulation of AQP4 internalization PMID: 27788222
    17. data propose that AQP4 belongs to the growing list of NH3-permeable water channels. PMID: 27435677
    18. this study shows that upregulation of AQP4 is associated with acute lung injury induced by intestinal ischemia reperfusion PMID: 27236300
    19. Age-related modulations of AQP4 and Caveolin-1 in the hippocampus predispose the toxic effect of Phoneutria nigriventer spider venom. PMID: 27886057
    20. AQP4 interference reduces CBT edema formation, and ADC value may predict TBI severity. PMID: 27930615
    21. The findings indicate that mRNA expression of AQP4 varies with age. PMID: 27525904
    22. AQP4 inhibition could weaken excitotoxicity in epileptogenesis by reducing proinflammatory cytokines in the hippocampus. PMID: 27706789
    23. The AQP4 protein in Sprague-Dawley (SD) rats was expressed after experimental intraventricular hemorrhage to investigate the possible pathogenesis of chronic posthemorrhagic hydrocephalus. PMID: 27188736
    24. Ulinastatin can effectively reduce the cardiac function damage caused by cardiopulmonary resuscitation, which could be related with the increased SSAT2 and decreased AQP4. PMID: 26617791
    25. The alteration of DG expression could regulate that of AQP4 expression after Traumatic Brain Injury . PMID: 26583111
    26. AQP4 may be involved in the occurrence of traumatic brain edema via changes in expression, which act as a defense response. PMID: 26459070
    27. These results suggest that the stressful short-period and slow-paced treadmill exercise suppressed NHE1 and AQP4 expression resulting in the amelioration of brain edema at least partly via the moderate increase in plasma corticosterone levels. PMID: 26724742
    28. AQP4 is overexpressed in glial cells, resulting in their swelling and adversely affecting cerebral homeostasis. Once PMID: 26385393
    29. The down-regulation of AQP4 was associated with crush-induced optic nerve edema. PMID: 25479407
    30. Findings shows that focal brain injury results in an early development of brain edema involving both cytotoxic and vasogenic components, a persistent blood-brain barrier breakdown and a temporary decrease in aquaporin 4. PMID: 25770057
    31. Aquaporin 4 expression decreased in response to ischemia/reperfusion in the remote ischemic post-conditioning (RIPC) group. RIPC could attenuate focal cerebral ischemia/reperfusion injury, and was related with the down-regulation of AQP4 in astrocytes. PMID: 25907740
    32. Phosphorylation at serine 276 is necessary for AQP4 translocation in response to changes of tonicity. PMID: 26013827
    33. Results show that AQP4 is up-regulated in both the rat hippocampus and the cortex during neurodegenerative processes triggered by trimethyltin chloride PMID: 24912027
    34. a role for beta-dystroglycan in targeting and stabilizing AQP4 channel in astrocytic cells, which may be critical for water homeostasis in brain. PMID: 25545558
    35. results indicate that ERK1/2 pathway down-regulates AQP4 expression in scratch-injured astrocytes, and ERK1/2 pathway might be a novel therapeutic target in reversing the effects of astrocytes that contribute to traumatic brain edema PMID: 25800444
    36. AQP4 and Cx43 had two distinct mechanisms underlying brain edema formation within and surrounding the glioma. PMID: 25373717
    37. Hypoxia triggers cortical release of CRF, which acts on CRFR1 to trigger signaling of cAMP/PKA in cortical astrocytes, leading to activation of AQP4 and cerebral edema. PMID: 25146699
    38. Lactuside B protected against cerebral edema and nerve cell damage caused by cerebral ischemic injury by decreasing the expression of AQP4 and TRPM7 mRNAs in the cerebral cortex of rats. PMID: 24817288
    39. Phosphorylation at COOH-terminal serine residues is not required for proper plasma localization of AQP4. PMID: 25231107
    40. MMP2/AQP4/AQP9 mRNA and protein expression. PMID: 24828425
    41. It is an water channel protein, involves in brain edema augmentation under ethanol consumption. PMID: 25223087
    42. The simulation studies showed a continuous movement of water in a single file across pore of unphosphorylated as well as phosphorylated rAQP4. PMID: 23651078
    43. The co-immunoprecipitation experiments showed a direct interaction between AQP4 and CFTR in cultured rat Sertoli cells. PMID: 24657265
    44. The correlations between ADC values and the brainstem AQP4 expression at different time points suggest that AQP4 expression follows an adaptative profile to the severity of brain edema. PMID: 24282821
    45. AQP4 expression was down-regulated during the angioedema attack, but AQP4 expression was upregulated during intracellular edema. PMID: 24238521
    46. Increased aquaporin-4 expression is observed in rat spinal cord following post-traumatic syringomyelia. PMID: 23441695
    47. The data of this study indicate a lack of phosphorylation of Ser(111) and of phosphorylation-dependent gating of AQP4. PMID: 23616425
    48. Mislocalization of AQP4 in a model of temporal lobe epilepsy reflects a loss of astrocyte polarization. PMID: 23357720
    49. AQP4 and NPY may be involved in the pathogenesis of intestinal dysfunction after traumatic brain injury. Increased NPY levels may be responsible for intestinal ischemia and hypoxia, and AQP4 may play an important role in intestinal edema. PMID: 23622727
    50. Silencing of AQP4 expression blocks astrocyte swelling after oxygen-glucose deprivation and reoxygenation. PMID: 23142737

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  • 亞細胞定位：
    Cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein. Endosome membrane. Cell membrane, sarcolemma; Multi-pass membrane protein. Cell projection.
  • 蛋白家族：
    MIP/aquaporin (TC 1.A.8) family
  • 組織特異性：
    Detected in cerebellum. Detected on pericapillary astrocyte endfeet in cerebellum, and in skeletal muscle. Detected in glial lamellae in the hypothalamus (at protein level). Abundant in mature brain cortex, cerebellum and spinal cord. Highly expressed in
  • 數據庫鏈接：