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Recombinant Rat Gap junction alpha-1 protein (Gja1)

  • 中文名稱：
    大鼠Gja1重組蛋白
  • 貨號(hào)：
    CSB-CF009443RA
  • 規(guī)格：
  • 來(lái)源：
    in vitro E.coli expression system
  • 其他：

產(chǎn)品詳情

  • 基因名：
  • Uniprot No.：
  • 別名：
    Gja1; Cxn-43; Gap junction alpha-1 protein; Connexin-43; Cx43; Gap junction 43 kDa heart protein
  • 種屬：
    Rattus norvegicus (Rat)
  • 蛋白長(zhǎng)度：
    Full Length of Mature Protein
  • 表達(dá)區(qū)域：
    2-382
  • 氨基酸序列
    GDWSALGKLLDKVQAYSTAGGKVWLSVLFIFRILLLGTAVESAWGDEQSAFRCNTQQPGC ENVCYDKSFPISHVRFWVLQIIFVSVPTLLYLAHVFYVMRKEEKLNKKEEELKVAQTDGV NVEMHLKQIEIKKFKYGIEEHGKVKMRGGLLRTYIISILFKSVFEVAFLLIQWYIYGFSL SAVYTCKRDPCPHQVDCFLSRPTEKTIFIIFMLVVSLVSLALNIIELFYVFFKGVKDRVK GRSDPYHATTGPLSPSKDCGSPKYAYFNGCSSPTAPLSPMSPPGYKLVTGDRNNSSCRNY NKQASEQNWANYSAEQNRMGQAGSTISNSHAQPFDFPDDNQNAKKVAAGHELQPLAIVDQ RPSSRASSRASSRPRPDDLEI
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標(biāo)簽：
    N-terminal 10xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract. May play a role in cell growth inhibition through the regulation of NOV expression and localization. Plays an essential role in gap junction communication in the ventricles.
  • 基因功能參考文獻(xiàn)：
    1. Intracellular Ca2+ signals and Cx43 hemichannel opening participate as key steps in the regulation of Thy-1-induced astrocyte migration. PMID: 30049932
    2. Multiple Phosphorylation-Dependent Roles of the Carboxyl Terminus in Cx43 Channel Gating and Permeation are studied. PMID: 29867029
    3. beta;-catenin directly interacts with the Cx43 carboxyl-terminal domain. PMID: 29882937
    4. Treatment with different concentrations of PDGF increased the levels of phosphorylated protein kinase B (AKT), beta-catenin, and Cx43, whereas inhibition of PDGFR or activation of phosphatidylinositol 3 kinase (PI3K)/AKT signaling altered b-catenin and Cx43 expression. PMID: 29174980
    5. Cx43 expression was significant upregulated in glomeruli of ORG rats compared to the control group. PMID: 29246788
    6. Data suggest that mitochondrial Cx43 is involved in apoptotic process in cardiomyocytes (using chemical model of cell hypoxia in cardiomyocyte cell line); mitochondrial Cx43 appears to play crucial role in cytoprotection/cardioprotection. PMID: 29155206
    7. These findings indicate that Cx43 positively modulates osteogenic differentiation of Bone Marrow Stromal Cells by up-regulating GSK-3beta/beta-catenin signaling pathways. PMID: 29763908
    8. the protein expression levels of hippocampal Cx36 and Cx43 during epileptogenesis in the pilocarpine model of epilepsy, were examined. PMID: 28042145
    9. Cx43 in the gap junctional channel may regulate lymphocyte activation and inflammatory cytokine production. PMID: 29115377
    10. the ERK/MAPK signaling pathway may be involved in elevating cardiac Cx43 expression levels under glucose deprivation in H9c2 cells PMID: 29115504
    11. The osteogenic effect of dorsal root ganglion neurons on mesenchymal stem cells is mediated through the regulation of Cx43 and N-cadherin expression and activation of the canonical/beta-catenin Wnt signaling pathway. PMID: 29238079
    12. In vitro gain and loss of function analyses showed overexpression of Connexin 32 and knockdown of Connexin 43 promoted hepatocytes differentiation from hepatic progenitor cells. PMID: 29116012
    13. Potentiation of Amitriptyline Anti-Hyperalgesic-Like Action By Astroglial Connexin 43 Inhibition in Neuropathic Rats. PMID: 27941941
    14. WXKL protected the ultrastructure of the gap junctions and their constituent Cx43 by regulating miR-1 and PKC mediated signal transduction and increased the VFT significantly in the rat MI model. The results suggested that WXKL is an effective alternative medicine to prevent potentially lethal arrhythmia following MI. PMID: 29094045
    15. The results indicate that VEGF promotes endothelial progenitor cell differentiation and vascular repair through Cx43. PMID: 29065929
    16. Results indicate strain and species differences in Cx36 as well as Cx45 expression, possible compensatory processes after loss of Cx36 expression and localization of Cx45 to both neuronal and Bergmann glial gap junctions. Expression of both Cx43 and Cx45 in Bergmann glia of rat may contribute to the complex properties of junctional coupling between these cells and perhaps to their reported coupling with Purkinje cells. PMID: 28561933
    17. myocardial Cx43 is most likely implicated in potentially salutary responses to acute heart injury PMID: 28459162
    18. Data suggest that ischemic preconditioning (IPC)-induced cardioprotection involves increased nitric oxide (NO)production, protein kinase C epsilon (PKC-epsilon) translocation, connexin43 (Cx43) phosphorylation, and chemical gap junction (GJ) uncoupling. PMID: 27655723
    19. recruitment of Csk and PTEN to the region between residues 266 and 283 within the C-terminus of Cx43 leads to c-Src inhibition in glioma cells and astrocytes PMID: 27391443
    20. Hypoxic postconditioning with siRNA targeting connexin 43 or the reactive oxidative species scavengers superoxide dismutase plus catalase significantly prevented reactive oxidative specie generation and cardioprotection. These data strongly supported the involvement of connexin 43 in HPC cardioprotection. PMID: 29179175
    21. Histological analysis revealed extravasation of erythrocytes and immunohistochemical analysis alteration of the pattern of the gap-junction protein connexin 43. PMID: 29121655
    22. Ezrin associates with Cx43 (in complex with ZO-1) which places PKA in proximity to Cx43, enabling its phosphorylation and gap junction opening. PMID: 28077322
    23. our study indicates that Cx43 hemichannels promote the disassembly of cell junctions through regulation of intracellular oxidative status. PMID: 27567473
    24. Cx43 and Smad1 promote BMP2-induced cartilage differentiation of bone marrow mesenchymal stem cells and inhibit osteoblast differentiation, which provide a new strategy for cartilage tissue engineering using exogenous Cx43 and Smad1. PMID: 28697500
    25. Cx43 hemichannels contribute to smooth muscle cell Ca(2+ )dynamics and contractility, by facilitating Ca(2+ )entry, ATP release, and purinergic signalling. PMID: 27677282
    26. In conclusion, AVP-mediated vascular contractile effects are endothelium and myoendothelial gap junction independent. Gap junctions between VSMCs, gap junctional communication, and Cx43 phosphorylation at Ser(262) play important roles in the vascular effects of AVP. RhoA/ROCK, but not PKC, is involved in this process. PMID: 28974518
    27. Based on these findings, we propose that Cx43 was the Cx isoform involved in MEGJ-mediated VEC-dependent regulation of Ang-2, which induces iNOS protein expression and vascular hyporeactivity after hypoxia. PMID: 28637680
    28. High levels of SGSM3 appeared to induce the turnover of Cx43 through lysosomal degradation in myocardial infarcted rat hearts. PMID: 28716730
    29. a plaque accretion defect as the primary manifestation of myosin VI loss in Cx43 homeostasis, is reported. PMID: 28096472
    30. Study demonstrated that recombinant tissue plasminogen activator-induced hemorrhagic transformation in cerebral ischemia is closely related to an increase in p-Cx43 through the activation of the PI3K and ERK pathways PMID: 27138645
    31. Cx43 plays a proapoptotic role in cisplatin-induced auditory cell death in both junctional and nonjunctional conditions. Targeting the Cx-mediated signaling control may be helpful in designing new therapeutic strategies for drug-induced ototoxicity PMID: 27122099
    32. Our findings demonstrate that Na-K-ATPase regulates intercellular communication in the vascular wall via cSrc-dependent Cx43 tyrosine phosphorylation. PMID: 28122732
    33. Cx43 expression role in intercellular electrical coupling PMID: 28034749
    34. These findings indicate that, like Src, Tyk2 can also inhibit gap junction communication by phosphorylating Cx43. PMID: 27235399
    35. internalization of Cx43 into the cytoplasm occurred during ischemia, which was partially mediated through endocytosis. PMID: 28424784
    36. FA also relaxed the mesenteric arterioles by reducing the expression of Cx43, which decreased blood pressure. Therefore, regulation of the expression of GJs may be a therapeutic target for the treatment of hypertension. PMID: 27748857
    37. There is an inverse relationship between expression of cardiac Cx43 and the levels of circulating thyroid hormones. PMID: 27643942
    38. ischemic postconditioning can inhibit the up-regulation of miRNA-1 induced by ischemia-reperfusion and that the down-regulation of miRNA-1 can prevent the decrease and redistribution of Cx43 PMID: 28081514
    39. Neuronal Cx36 plays a role in rhythmic firing in substantia gelatinosa of rat neonatal dorsal horn in vitro. PMID: 27462070
    40. Thus, miR-206 and Cx43 are involved in the repair of exercise-induced muscle injury. PMID: 27706732
    41. Cx43 can communicate cylic AMP (cAMP) between cells and, more importantly, that the communicated cAMP is sufficient to impact signal transduction cascades and the expression of key bone effector molecules between interconnected cells. PMID: 27156839
    42. Tiaogan Qingxin Granule reduced the myocardial infarct size, lowered the myocardial infarction rate, and increased Cx43 expression, possibly by increasing blood supply to the cardiac muscles. In conclusion, Tiaogan Qingxin Granule may be useful for treating ischemic PVB. PMID: 27323125
    43. This study demonstrated that altered Cx43 expression modulates bFGF expression, which correlates with prolactinoma development. PMID: 27078698
    44. the propagation of satellite glial cell activation throughout the trigeminal ganglion via gap junctions, which are composed of connexin 43, plays a pivotal role in ectopic mechanical hypersensitivity in whisker pad skin following inferior alveolar nerve injury. PMID: 27030716
    45. Phosphorylation of connexin43 serine-368 is necessary to maintain conduction velocity during metabolic stress. PMID: 26459193
    46. These data indicate a fine regulation of the gap junction channel make-up in function of the type and the ratio of co-expressed connexins that specifically regulates chemical and electrical coupling. PMID: 26550940
    47. Cx43 is involved in the development of vascular RS. PMID: 26398676
    48. MG132 may be used to prevent degradation of Cx43 in heart failure, and thus may prevent Cx43-mediated arrhythmia. PMID: 26398314
    49. Findings suggest that overexpression of connexin 43 (Cx43) reinitiated spermatogenesis at least through the steps of meiosis to generate round spermatids in testes. PMID: 26678449
    50. Ovariectomized rats showed frequent bladder contraction and increased connexin-43 expression without changes in M2 and M3 receptor expression. PMID: 25647175

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  • 亞細(xì)胞定位：
    Cell membrane; Multi-pass membrane protein. Cell junction, gap junction. Endoplasmic reticulum.
  • 蛋白家族：
    Connexin family, Alpha-type (group II) subfamily
  • 組織特異性：
    Detected in ventricle and atrium (at protein level).
  • 數(shù)據(jù)庫(kù)鏈接：