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  4. Recombinant Xanthomonas campestris pv. campestris Sensory/regulatory protein RpfC (rpfC)

Recombinant Xanthomonas campestris pv. campestris Sensory/regulatory protein RpfC (rpfC)

  • 中文名稱：
    野生彎曲黃單胞菌rpfC重組蛋白
  • 貨號：
    CSB-CF314618XAF
  • 規(guī)格：
  • 來源：
    in vitro E.coli expression system
  • 其他：

產(chǎn)品詳情

  • 基因名：
    rpfC
  • Uniprot No.：
  • 別名：
    rpfC; XC_2333; Sensory/regulatory protein RpfC
  • 種屬：
    Xanthomonas campestris pv. campestris (strain 8004)
  • 蛋白長度：
    full length protein
  • 表達(dá)區(qū)域：
    1-726
  • 氨基酸序列
    MKSPLPWLKRRLSGRADSEHAQNLIRIIITTLFISYLGWRYQHTHGDTLMATWLILVGEL LVSLGLMVAILLRPQVSHTRRLIGMLLDYTCTGAIMAIQGEPASPLYAVCMWVTIGNGLR YGSNYLRAATAMGSLCFLGAILISPYWKANPYLSWGLLLGLIAVPLYFDSLLRAMTRAVR EARHANQAKSRFLANMSHEFRTPLNGLSGMTEVLATTRLDAEQKECLNTIQASARSLLSL VEEVLDISAIEAGKIRIDRRDFSLREMIGSVNLILQPQARGRRLEYGTQVADDVPDLLKG DTAHLRQVLLNLVGNAVKFTEHGHVLLRVTRVSGSAEDAVRLRFDVEDTGIGVPMDMRPR LFEAFEQADVGLSRRYEGTGLGTTIAKGLVEAMGGSIGFKENQPSGSVFWFELPMAIGEP LKSSTVRVPTGALVDAPEELESSNIIAFSNPFLRHRARVRSMRMLVADDHEANRMVLQRL LEKAGHKVLCVNGAEQVLDAMAEEDYDAVIVDLHMPGMNGLDMLKQLRVMQASGMRYTPV VVLSADVTPEAIRACEQAGARAFLAKPVLAAKLLDTLADLAVSTRQLATPATTVQVATSF EGVLDSSVLDELAALGMGEEFERQFVRQCLDDAQNCVGDIERDGTCSDWEQLRESAHALR GVASNLGLAQVASSGGELMRMADWQLQAEWRLRLSTLREQLKAGKDALDARVQGVKDGEC SPRSNE
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標(biāo)簽：
    N-terminal 10xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Hybrid sensor kinase that regulates diverse biological functions through two distinct molecular mechanisms. At low cell density, the extracellular concentration of the diffusible signaling factor (DSF) is below a threshold, and unphosphorylated RpfC is involved in the negative regulation of DSF synthesis, via direct interaction with the DSF synthase RpfF. Interaction prevents synthesis of DSF, which remains at a basal level. This activity does not involve the phosphorelay mechanism and is not dependent on RpfG. Is also member of the two-component regulatory system RpfG/RpfC, which is involved in the perception and response to DSF, which is essential for cell-cell signaling. At high cell density, the level of extracellular DSF increases and binding of DSF to the sensor region of RpfC causes autophosphorylation of RpfC, which results in the release of RpfF and the activation of RpfG via a four-step phosphorelay. Activation of RpfG leads to the positive regulation of biofilm dispersal and the production of virulence factors.
  • 亞細(xì)胞定位：
    Cell inner membrane; Multi-pass membrane protein.
  • 數(shù)據(jù)庫鏈接：

    KEGG: xcb:XC_2333

    STRING: 314565.XC_2333