Your Good Partner in Biology Research

自身抗體科研ELISA試劑盒

自身抗體ELISA試劑盒

免疫系統(tǒng)高度精密且復(fù)雜,其主要功能除了能夠保護(hù)機(jī)體免受外部病原體侵害之外,還能識別和清除機(jī)體內(nèi)部的凋亡細(xì)胞和腫瘤細(xì)胞,通過自身免疫耐受和免疫調(diào)節(jié)來維持機(jī)體內(nèi)環(huán)境的穩(wěn)定。然而,一旦免疫系統(tǒng)出現(xiàn)故障,它就會錯誤地攻擊自身健康的細(xì)胞、組織和器官,誘發(fā)自身免疫反應(yīng),從而產(chǎn)生某些病理性的自身抗體(Autoantibody)。與人體內(nèi)天然存在的某些自身抗體不同,這類病理性自身抗體與自身抗原的親和力極高,可引起自身組織的病理性損傷并誘發(fā)自身免疫性疾病。

CUSABIO致力于為研究人員提供高精確性、高靈敏度的自身抗體ELISA試劑盒,涵蓋抗dsDNA抗體、抗核小體抗體、胰島素抗體和類風(fēng)濕因子抗體等多種常見的IgG或IgM型自身抗體,有助于研究自身免疫性疾病的發(fā)病機(jī)制、生物標(biāo)志物的變化和新的腫瘤研究策略,為您的科學(xué)研究提供準(zhǔn)確可靠的數(shù)據(jù)支持,助力您在免疫學(xué)領(lǐng)域取得更多突破性進(jìn)展。

點擊跳轉(zhuǎn)至完整產(chǎn)品目錄

1. 自身抗體

自身抗體(Autoantibody,AAB)是指人體免疫系統(tǒng)產(chǎn)生的針對自身組織的抗體,通常包括天然自身抗體和病理性自身抗體。天然自身抗體主要為 IgM型,由未突變的 V(D)J 基因編碼,對自身抗原親和力較低。天然自身抗體提供了機(jī)體抵御感染的第一道防線,具有一定的生理功能,比如維持細(xì)胞功能和調(diào)節(jié)免疫系統(tǒng)穩(wěn)態(tài)。相比之下,由體細(xì)胞突變的、和自身抗原有著高親和力的 IgG 型自身抗體則反映了一種病理過程,可能導(dǎo)致正常細(xì)胞被清除、抗原-受體的信號傳導(dǎo)或細(xì)胞效應(yīng)功能的紊亂,從而引起自身組織的病理性損傷。

2. 自身免疫性疾病及其類型

自身免疫性疾病(Autoimmune Disease, AID)是指免疫系統(tǒng)對自身細(xì)胞或成分產(chǎn)生免疫應(yīng)答而引起的疾病狀態(tài)。自身免疫性疾病的具體原因尚不完全清楚,可能與遺傳因素、環(huán)境因素以及免疫系統(tǒng)的異常反應(yīng)等多種因素有關(guān)。目前已知的自身免疫性疾病超過100種,主要分為器官特異性AD和系統(tǒng)性AD兩種類型:

(1)器官特異性自身免疫疾?。∣rgan-Specific Autoimmune Diseases):這類疾病的免疫反應(yīng)主要針對特定的器官或組織。常見的特異性自身免疫疾病包括:自身免疫性甲狀腺疾病、銀屑病、多發(fā)性硬化癥、自身免疫性肝炎、重癥肌無力、尋常天皰瘡、I型糖尿病等。

(2)系統(tǒng)性自身免疫疾?。⊿ystemic Autoimmune Diseases):相比之下,系統(tǒng)性自身免疫疾病涉及多個器官和組織,其免疫反應(yīng)波及全身多個系統(tǒng)。一些常見的系統(tǒng)性自身免疫疾病包括:系統(tǒng)性紅斑狼瘡、類白塞病、類風(fēng)濕性關(guān)節(jié)炎、炎癥性腸病、硬皮病、系統(tǒng)性血管炎等。

自身免疫性疾病的誘因和常見癥狀

圖1. 自身免疫性疾病的誘因和常見癥狀 [1]

3. 自身抗體與自身免疫性疾病

在某些自身免疫性疾病中,自身抗體可能在疾病發(fā)作前就存在,且表現(xiàn)出顯著的特異性,因此可作為關(guān)鍵生物標(biāo)志物助力相關(guān)疾病的治療研究。在重癥肌無力或天皰瘡等器官特異性自身免疫性疾病中,自身抗體直接結(jié)合并損傷靶器官[2]。在血管炎、腎小球腎炎以及系統(tǒng)性紅斑狼瘡等系統(tǒng)性自身免疫性疾病中,自身抗體與游離分子(例如磷脂)以及細(xì)胞表面和核蛋白抗原發(fā)生反應(yīng),形成致病性抗原-抗體(免疫)復(fù)合物。這些自身抗體通過參與補(bǔ)體的 FcγR 激活以及 Toll 樣受體的內(nèi)化和激活來損傷組織和器官[3]。

4. 自身抗體與腫瘤

在腫瘤發(fā)展的早期階段,盡管腫瘤抗原在血清中很難檢測到,但人體免疫系統(tǒng)會產(chǎn)生與腫瘤相關(guān)的自身抗體,這些自身抗體有望成為癌癥早期診斷的有希望的生物標(biāo)志物。P. Budde等人[4]對接受了prostvac 和 ipilimumab治療的前列腺癌患者進(jìn)行了自身抗體分析,研究發(fā)現(xiàn),一些AAB與治療后出現(xiàn)的免疫相關(guān)不良事件(irAEs)有關(guān),而另一些AAB則可能有助于抵消炎癥分子的作用,從而減少irAEs的發(fā)生。此外,許多抗TAA自身抗體被驗證能夠作為肺癌、肝細(xì)胞癌等癌癥早期檢測的潛在生物標(biāo)志物[5,6]。

5. CUSABIO的自身抗體相關(guān)ELISA試劑盒列表

貨號 產(chǎn)品名稱 靶點
CSB-E04868h Human anti-cardiolipin antibody(IgG) ELISA Kit ACA Ab (IgG)
CSB-E08646m Mouse anti-cardiolipin antibody (IgG) ELISA Kit ACA Ab (IgG)
CSB-E09077h Human anti-cyclic citrullinated peptide antibody (anti-CCP antibody) ELISA Kit ACCPA
CSB-E08371h Human acetylcholine receptor antibody,AChRab ELISA Kit AChR Ab
CSB-E08691h Human anti-endothelial cell antibody,AECA ELISA Kit AECA
CSB-E09697h Human anti-fibrillarin antibody,AFA/snoRNP/U3RNP ELISA Kit AFA/snoRNP/U3RNP
CSB-E12912m Mouse anti-nuclear Antibody (IgG) ELISA Kit ANA Ab (IgG)
CSB-EQ027743MO Mouse Anti-Cyclic Citrullinated Peptide Antibody (Anti-CCP-antibody) ELISA kit Anti-CCP-Ab
CSB-E14342m Mouse anti-nucleosome antibody (IgG) ELISA Kit AnuA Ab (IgG)
CSB-E13568h Human Aquaporin 4 Antibody,AQP-4 Ab ELISA Kit AQP-4 Ab
CSB-E05118h Human anti-sperm antibody,AsAb ELISA Kit AsAb
CSB-E09419h Human Anti-smooth muscle antibody,ASMA ELISA Kit ASMA
CSB-E09082h Human anti-zona pellucida antibody,aZP ELISA Kit AZPAb
CSB-E09165h Human Anti-Complement 1q antibody(anti-C1q-antibody)ELISA Kit C1q Ab
CSB-E13830r Rat Anti-Cyclic Citrullinated Peptide Antibody(Anti-CCP-antibody)ELISA Kit CCP Ab
CSB-E12990h Human anti-thrombopoietin receptor(C-MPL) autoantibody IgG ELISA kit C-MPL/TPOR auto-Ab (IgG)
CSB-E09063h Human anti-cytomegalovirus(CMV)  antibody (IgG) ELISA Kit CMV Ab (IgG)
CSB-E11194m Mouse anti-double stranded DNA antibody (IgG) ELISA Kit dsDNA Ab (IgG)
CSB-E04911h Human anti-double stranded DNA(dsDNA) antibody(IgG) ELISA Kit dsDNA Ab (IgG)
CSB-E08655m Mouse glutamic acid decarboxylase (GAD) autoantibody (IgG) ELISA Kit GAD au-Ab (IgG)
CSB-E15971h Human glutamic acid decarboxylase 65(GAD65) antibody (IgG) ELISA Kit GAD65 Ab (IgG)
CSB-EQ027671HU Human anti glial fibrillary acidic protein (GFAP) autoantibody ELISA kit GFAP auto-Ab
CSB-E11438h Human anti-Granulocyte-Macrophage Colony Stimulating Factor(GM-CSF) antibody ELISA Kit GM-CSF Ab
CSB-E09205h Human insulin autoantibodies,IAA ELISA Kit IAA
CSB-E10369m Mouse insulin autoantibodies(IAA) ELISA Kit IAA
CSB-E04972h Human islet cell antibody,ICA ELISA Kit ICA
CSB-E08812m Mouse islet cell antibody,ICA ELISA Kit ICA
CSB-E15753h Human Phosphatidic Acid Antibody(IgM) ELISA Kit IgM
CSB-EQ027171HU Human anti-IL1 autoantibody ELISA kit IL1 auto-Ab
CSB-E09568h Human anti-insulin receptor antibody,AIRA ELISA Kit InsR Ab
CSB-E08676m Mouse myeloperoxidase-antineutrophil cytoplasmic antibody ELISA kit MAC Ab
CSB-E04787h Human myelin basic protein(MBP) antibody ELISA Kit MBP Ab
CSB-E09565h Human anti-mutated citrullinated vimentin (MCV) antibody ELISA Kit MCV Ab
CSB-E08675r Rat myeloperoxidase-antineutrophil cytoplasmic antibody(MPO-ANCA) ELISA kit MPO-ANCA
CSB-E16639h Human Muscle Skeletal Receptor Tyrosine Kinase Antibody(MUSK Ab) ELISA Kit MUSK Ab
CSB-E13555h Human N-methyl-D-aspartic acid (NMDA) antibody (IgG) ELISA Kit NMDA Ab (IgG)
CSB-E12816h Human anti-platelet antibody (anti-PA Ab)ELISA Kit PA Ab
CSB-E15044h Human Phosphatidylethanolamine Antibody(IgG) ELISA Kit PE Ab (IgG)
CSB-E15752h Human Phosphatidylethanolamine Antibody(IgM) ELISA Kit PE Ab (IgM)
CSB-E13666r Rat Rheumatoid Factor(RF) ELISA Kit RF
CSB-EQ027571HU Human rheumatoid factor (RF) antibody (IgG) ELISA kit RF Ab (IgG)
CSB-E13357h Human anti-ryanodine receptor calcium release channel antibodies ELISA Kit RRCRC Ab
CSB-EQ027203HU Human anti streptokinase(SK) antibody ELISA kit SK Ab
CSB-E15976m Mouse anti-Smith antibody ELISA Kit Sm Ab
CSB-E11874h Human anti-Survivin(Surv) antibody ELISA Kit Surv Ab
CSB-E13356h Human titin antibody(IgG) ELISA Kit titin Ab (IgG)
CSB-E09345h Human voltage-gated calcium channel antibody(IgG) ELISA Kit VGCC Ab (IgG)

參考文獻(xiàn):

[1] https://www.niehs.nih.gov/health/topics/conditions/autoimmune/index.cfm

[2] Lazaridis K, Tzartos S J. Autoantibody Specificities in Myasthenia Gravis; Implications for Improved Diagnostics and Therapeutics. Front Immunol. 2020 Feb 14;11:212.

[3] Elkon K, Casali P. Nature and functions of autoantibodies. Nat Clin Pract Rheumatol. 2008 Sep;4(9):491-8.

[4] Budde P, Marte J, Zucht H, et al. THU0043 Autoantibody profiling in prostvac and ipilimumab treated prostate cancer patients reveals potential biomarkers of immune-related adverse events. Annals of the Rheumatic Diseases. 2018;77:247.

[5] Wang K, Li M, Qin J, et al. Serological Biomarkers for Early Detection of Hepatocellular Carcinoma: A Focus on Autoantibodies against Tumor-Associated Antigens Encoded by Cancer Driver Genes. Cancers. 2020; 12(5):1271.

[6] Jiang D, Wang Y, Liu M, et al. A panel of autoantibodies against tumor-associated antigens in the early immunodiagnosis of lung cancer. Immunobiology, 2020, 225(1): 151848.