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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Salmonella schwarzengrund Protein tsgA (tsgA), partial CSB-YP465903SWV
CSB-EP465903SWV
CSB-BP465903SWV
CSB-MP465903SWV
CSB-EP465903SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Fe/S biogenesis protein NfuA (nfuA) CSB-YP465904SWV
CSB-EP465904SWV
CSB-BP465904SWV
CSB-MP465904SWV
CSB-EP465904SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Lipid-A-disaccharide synthase (lpxB) CSB-YP465905SWV
CSB-EP465905SWV
CSB-BP465905SWV
CSB-MP465905SWV
CSB-EP465905SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Phosphoribosylglycinamide formyltransferase 2 (purT) CSB-YP465906SWV
CSB-EP465906SWV
CSB-BP465906SWV
CSB-MP465906SWV
CSB-EP465906SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund D-cysteine desulfhydrase (dcyD) CSB-YP465907SWV
CSB-EP465907SWV
CSB-BP465907SWV
CSB-MP465907SWV
CSB-EP465907SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP465908SWV
CSB-EP465908SWV
CSB-BP465908SWV
CSB-MP465908SWV
CSB-EP465908SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Gamma-glutamyl phosphate reductase (proA) CSB-YP465909SWV
CSB-EP465909SWV
CSB-BP465909SWV
CSB-MP465909SWV
CSB-EP465909SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Acyl carrier protein phosphodiesterase (acpH) CSB-YP465910SWV
CSB-EP465910SWV
CSB-BP465910SWV
CSB-MP465910SWV
CSB-EP465910SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Cobalt transport protein CbiN (cbiN), partial CSB-YP465911SWV1
CSB-EP465911SWV1
CSB-BP465911SWV1
CSB-MP465911SWV1
CSB-EP465911SWV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella schwarzengrund Nucleoid occlusion factor SlmA (slmA) CSB-YP465912SWV
CSB-EP465912SWV
CSB-BP465912SWV
CSB-MP465912SWV
CSB-EP465912SWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP465913FMK
CSB-EP465913FMK
CSB-BP465913FMK
CSB-MP465913FMK
CSB-EP465913FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus UPF0297 protein Sez_1866 (Sez_1866) CSB-YP465914FMK
CSB-EP465914FMK
CSB-BP465914FMK
CSB-MP465914FMK
CSB-EP465914FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP465915FMK
CSB-EP465915FMK
CSB-BP465915FMK
CSB-MP465915FMK
CSB-EP465915FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus 30S ribosomal protein S6 (rpsF) CSB-YP465916FMK
CSB-EP465916FMK
CSB-BP465916FMK
CSB-MP465916FMK
CSB-EP465916FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP465917FMK
CSB-EP465917FMK
CSB-BP465917FMK
CSB-MP465917FMK
CSB-EP465917FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus Serine--tRNA ligase (serS), partial CSB-YP465918FMK
CSB-EP465918FMK
CSB-BP465918FMK
CSB-MP465918FMK
CSB-EP465918FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus Galactose-6-phosphate isomerase subunit lacB (lacB) CSB-YP465919FMK
CSB-EP465919FMK
CSB-BP465919FMK
CSB-MP465919FMK
CSB-EP465919FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus Phosphopantetheine adenylyltransferase (coaD) CSB-YP465920FMK
CSB-EP465920FMK
CSB-BP465920FMK
CSB-MP465920FMK
CSB-EP465920FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus Bifunctional protein FolD (folD) CSB-YP465921FMK
CSB-EP465921FMK
CSB-BP465921FMK
CSB-MP465921FMK
CSB-EP465921FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equi subsp. zooepidemicus Glucosamine-6-phosphate deaminase (nagB) CSB-YP465922FMK
CSB-EP465922FMK
CSB-BP465922FMK
CSB-MP465922FMK
CSB-EP465922FMK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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