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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant 30S ribosomal protein S21 (rpsU) CSB-YP481700DPP
CSB-EP481700DPP
CSB-BP481700DPP
CSB-MP481700DPP
CSB-EP481700DPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant GTPase obg (obg) CSB-YP481701DPP
CSB-EP481701DPP
CSB-BP481701DPP
CSB-MP481701DPP
CSB-EP481701DPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L27 (rpmA) CSB-YP481702DPP
CSB-EP481702DPP
CSB-BP481702DPP
CSB-MP481702DPP
CSB-EP481702DPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dihydroorotate dehydrogenase B (NAD (+)), catalytic subunit CSB-YP481703DPP
CSB-EP481703DPP
CSB-BP481703DPP
CSB-MP481703DPP
CSB-EP481703DPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bifunctional protein FolD (folD) CSB-YP481704DPP
CSB-EP481704DPP
CSB-BP481704DPP
CSB-MP481704DPP
CSB-EP481704DPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP481705DPP
CSB-EP481705DPP
CSB-BP481705DPP
CSB-MP481705DPP
CSB-EP481705DPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gamma-glutamyl phosphate reductase (proA) CSB-YP481706DPP
CSB-EP481706DPP
CSB-BP481706DPP
CSB-MP481706DPP
CSB-EP481706DPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP481707DPP
CSB-EP481707DPP
CSB-BP481707DPP
CSB-MP481707DPP
CSB-EP481707DPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Proteasome subunit alpha (psmA) CSB-YP481708TMR
CSB-EP481708TMR
CSB-BP481708TMR
CSB-MP481708TMR
CSB-EP481708TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus 50S ribosomal protein L23P (rpl23p) CSB-YP481709TMR
CSB-EP481709TMR
CSB-BP481709TMR
CSB-MP481709TMR
CSB-EP481709TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Ribonuclease P protein component 1 (rnp1) CSB-YP481710TMR
CSB-EP481710TMR
CSB-BP481710TMR
CSB-MP481710TMR
CSB-EP481710TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Adenylate kinase (adkA) CSB-YP481711TMR
CSB-EP481711TMR
CSB-BP481711TMR
CSB-MP481711TMR
CSB-EP481711TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus 50S ribosomal protein L18e (rpl18e) CSB-YP481712TMR
CSB-EP481712TMR
CSB-BP481712TMR
CSB-MP481712TMR
CSB-EP481712TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Isopentenyl-diphosphate delta-isomerase (fni) CSB-YP481713TMR
CSB-EP481713TMR
CSB-BP481713TMR
CSB-MP481713TMR
CSB-EP481713TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus UPF0278 protein TON_0169 (TON_0169) CSB-YP481714TMR
CSB-EP481714TMR
CSB-BP481714TMR
CSB-MP481714TMR
CSB-EP481714TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus UPF0095 protein TON_0188 (TON_0188) CSB-YP481715TMR
CSB-EP481715TMR
CSB-BP481715TMR
CSB-MP481715TMR
CSB-EP481715TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus DNA-directed RNA polymerase subunit H (rpoH) CSB-YP481716TMR
CSB-EP481716TMR
CSB-BP481716TMR
CSB-MP481716TMR
CSB-EP481716TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Ribosome biogenesis protein Nop10 (TON_0238) CSB-YP481717TMR
CSB-EP481717TMR
CSB-BP481717TMR
CSB-MP481717TMR
CSB-EP481717TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Probable tRNA pseudouridine synthase D (truD) CSB-YP481718TMR
CSB-EP481718TMR
CSB-BP481718TMR
CSB-MP481718TMR
CSB-EP481718TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus onnurineus Threonine--tRNA ligase (thrS), partial CSB-YP481719TMR
CSB-EP481719TMR
CSB-BP481719TMR
CSB-MP481719TMR
CSB-EP481719TMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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